Does red ginseng ameliorate liver damage caused by obstructive jaundice? : an experimental study

Year 2021, Volume: 4 Issue: 2, 233 - 239, 28.03.2021

Abdullah Durhan , Koray Koşmaz , Abdullah Şenlikci , Ender Ergüder , Marlen Süleyman , Mehmet Esat Duymuş , Yusuf Murat Bağ , Mevlüt Recep Pekcici , Mehmet Şeneş , İlknur Alkan Kuşabbi , Eylem Pınar Eser , Sema Hücümenoğlu

https://doi.org/10.32322/jhsm.900023

Cited By: 2

Abstract

Aim: This experimental study aimed to evaluate the hepatoprotective effect on obstructive jaundice (OJ) of oral red ginseng (RG) extract, which is known to have anti-inflammatory and antioxidant properties.
Methods: The rats were randomly separated into 3 groups of 10 rats: the sham group, the control group, and the treatment group. In Group 1 (sham), the common bile duct (CBD) was identified but no ligation or transection was performed. In Group 2 (control), the CBD was identified and ligation and transection were performed, but no treatment was given. In Group 3 (RG group), CBD ligation and transection were performed, then RG extract was administered via an orogastric tube at a dose of 100 mg/ kg/day for 10 days. After 10 days, blood samples were taken for biochemical analysis, and liver tissue samples for biochemical and histopathological analysis.
Results: Significantly higher serum albumin levels and lower serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined in the RG group than in the control group (p = 0.028, p = 0.001 and p = 0.034, respectively). In the oxidative stress parameters, malondialdehyde (MDA) levels and catalase (CAT) levels were significantly different between the RG group and control group (p = <0.001 for each). Total sulfhydryl (T-SH) was not at a statistically significant level, although it was high and approached the value of the sham group ( p = 0.076). In the histopathological evaluation, the RG group had statistically significantly lower scores in all parameters compared to the control group (p<0.05).
Conclusion: The results of this study showed that RG has a strong hepatoprotective effect as a result of its anti-inflammatory and antioxidant properties.

Keywords

Red ginseng, obstructive jaundice, anti-inflammatory properties, antioxidant properties, hepatoprotective effects

References

• 1. González-Chávez A, Elizondo-Argueta S, Gutiérrez-Reyes G, León-Pedroza JI. Pathophysiological implications between chronic inflammation and the development of diabetes and obesity. Cir Cir 2011; 79: 209-16.
• 2. Tracy RP. Emerging relationships of inflammation, cardiovascular disease and chronic diseases of aging. Int J Obes Relat Metab Disord 2003; 27: 29-34.
• 3. Allavena P, Germano G, Marchesi F, Mantovani A. Chemokines in cancer related inflammation. Exp Cell Res 2011; 317: 664-73.
• 4. Baeck C, Tacke F. Balance of inflammatory pathways and interplay of immune cells in the liver during homeostasis and injury. EXCLI J 2014; 13: 67-81.
• 5. Scott-Conner CE, Grogan JB. The pathophysiology of biliary obstruction and its effect on phagocytic and immune function. J Surg Res 1994; 57: 316-36.
• 6. Moran M, Oruc MT, Ozmen MM, et al. Effect of erythropoietin on oxidative stress and liver injury in experimental obstructive jaundice. Eur Surg Res 2009; 43: 228-34.
• 7. KoŞmaz K, Durhan A, SÜleyman M, et al. The Effect of Ankaferd Blood Stopper on Liver Damage in Experimental Obstructive Jaundice. Turk J Med Sci 2020. doi:10.3906/sag-2007-298.
• 8. Kismet K, Sabuncuoglu MZ, Kilicoglu SS, et al. Effect of propolis on oxidative stress and histomorphology of liver tissue in experimental obstructive jaundice. Eur Surg Res 2008; 41: 231-7.
• 9. Ahuja A, Kim JH, Kim JH, Yi YS, Cho JY. Functional role of ginseng-derived compounds in cancer. J Ginseng Res 2018; 42: 248-54.
• 10. Huu Tung N, Uto T, Morinaga O, Kim YH, Shoyama Y. Pharmacological effects of ginseng on liver functions and diseases: a minireview. Evid Based Complement Alternat Med 2012; 2012: 173297.
• 11. Abdelfattah-Hassan A, Shalaby SI, Khater SI, El-Shetry ES, Abd El Fadil H, Elsayed SA. Panax ginseng is superior to vitamin E as a hepatoprotector against cyclophosphamide-induced liver damage. Complement Ther Med 2019; 46: 95-102.
• 12. Kim JH, Yi YS, Kim MY, Cho JY. Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responses and diseases. J Ginseng Res 2017; 41: 435-43.
• 13. Wasowicz W, Nève J, Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem 1993; 39: 2522-6.
• 14. Hadwan MH. Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochem 2018; 19: 7.
• 15. Taylan E, resmi h. The Analytical Performance of a Microplate Method for Total Sulfhydryl Measurement in Biological Samples. Turk J Biochem 2010; 35: 275-8.
• 16. Ishak KG. Pathologic features of chronic hepatitis. A review and update. Am J Clin Pathol 2000; 113: 40-55.
• 17. Padillo FJ, Cruz A, Navarrete C, et al. Melatonin prevents oxidative stress and hepatocyte cell death induced by experimental cholestasis. Free Radic Res 2004; 38: 697-704.
• 18. Chuang JH, Chang NK, Huang CC, et al. Biliary intervention augments chemotactic reaction and aggravates cholestatic liver injury in rats. J Surg Res 2004; 120: 210-8.
• 19. Cantürk NZ, Canturk Z, Utkan NZ, et al. Cytoprotective effects of alpha tocopherol against liver injury induced by extrahepatic biliary obstruction. East Afr Med J 1998; 75: 77-80.
• 20. Unal Y, Tuncal S, Kosmaz K, et al. The Effect of Calcium Dobesilate on Liver Damage in Experimental Obstructive Jaundice. J Invest Surg 2019; 32: 238-44.
• 21. Ljubuncic P, Tanne Z, Bomzon A. Evidence of a systemic phenomenon for oxidative stress in cholestatic liver disease. Gut 2000; 47: 710-6.
• 22. Dilektasli E, Ozmen MM, Gundogdu E, Dizen H, Besler HT, Ozogul C. The effects of obstructive jaundice on the brain: An experimental study. Asian J Surg 2016; 39: 155-63.
• 23. Lv Y, Yue J, Gong X, et al. Spontaneous remission of obstructive jaundice in rats: Selection of experimental models. Exp Ther Med 2018; 15: 5295-301.
• 24. Gillis CN. Panax ginseng pharmacology: a nitric oxide link? Biochem Pharmacol 1997; 54: 1-8.
• 25. Xu XF, Gao Y, Xu SY, et al. Remarkable impact of steam temperature on ginsenosides transformation from fresh ginseng to red ginseng. J Ginseng Res 2018; 42: 277-87.
• 26. Chang-Xiao L, Pei-Gen X. Recent advances on ginseng research in China. J Ethnopharmacol 1992; 36: 27-38.
• 27. Hasegawa H. Proof of the mysterious efficacy of ginseng: basic and clinical trials: metabolic activation of ginsenoside: deglycosylation by intestinal bacteria and esterification with fatty acid. J Pharmacol Sci 2004; 95: 153-7.
• 28. Kim KH, Lee D, Lee HL, Kim CE, Jung K, Kang KS. Beneficial effects of Panax ginseng for the treatment and prevention of neurodegenerative diseases: past findings and future directions. J Ginseng Res 2018; 42: 239-47.
• 29. Lü JM, Yao Q, Chen C. Ginseng compounds: an update on their molecular mechanisms and medical applications. Curr Vasc Pharmacol 2009; 7: 293-302.
• 30. Im DS. Pro-Resolving Effect of Ginsenosides as an Anti-Inflammatory Mechanism of Panax ginseng. Biomolecules 2020; 10: 444.
• 31. Karakus E, Karadeniz A, Simsek N, et al. Protective effect of Panax ginseng against serum biochemical changes and apoptosis in liver of rats treated with carbon tetrachloride (CCl4). J Hazard Mater 2011; 195: 208-13.
• 32. Hong SH, Suk KT, Choi SH, et al. Anti-oxidant and natural killer cell activity of Korean red ginseng (Panax ginseng) and urushiol (Rhus vernicifera Stokes) on non-alcoholic fatty liver disease of rat. Food Chem Toxicol 2013; 55: 586-91.
• 33. Abdel-Wahhab PM, Gamil K, Elkady A, el-nekeety A, Naguib K. Therapeutic Effects of Korean Red Ginseng Extract in Egyptian Patients with Chronic Liver Diseases. J Ginseng Res 2011; 35: 69-79.