EFFECTS OF PAMIDRONATE ADMINISTRATION ON TOOTH ERUPTION AND MANDIBULAR GROWTH IN NEW BORN RATS

Year 2017, Volume: 51 Issue: 1, 8 - 14, 02.01.2017

Çağrı Delilbaşı , İbrahim Tuncer Ediz Deniz Merva Soluk Tekkesin Vakur Olgaç , Kemal Şençift

Abstract

Purpose: Bisphosphonates are commonly used drugs in pediatric patients in the treatment of osteoporotic diseases and various types of cancers. The purpose of this study was to evaluate the effects of pamidronate administration on mandibular growth and tooth eruption in new born rats.

Materials and Methods: Forty Sprague Dawley rats were included in the study and divided into four groups as; 14^th day pamidronate group, 30^th day pamidronate group, 14^th day control group and 30^th day control group. Pamidronate groups were daily injected with 1.25 µg/g pamidronate disodium subcutaneously whereas control groups were injected with sterile saline. Eruption levels of lower incisor and molar teeth were assessed macroscopically. Mandibular growth was assessed by measuring reference points in cone beam tomography. Histological and histomorphometric examinations were performed under light microscope to evaluate tooth morphology and number of osteoclasts.

Results: Retardation in mandibular growth, decrease in number of osteoclasts, delay in tooth eruption, degeneration in both tooth morphology and structure were observed in the pamidronate groups compared to control groups.  

Conclusion: Pamidronate administration during growth and development stage may adversely affect tooth eruption and mandibular growth in new born rats.

Keywords

Bisphosphonate, mandibular growth; pamidronate; tooth eruption

References

• Hiraga T, Ninomiya T, Hosoya A, Nakamura H. Administration of the bisphosphonate zoledronic acid during tooth development inhibits tooth eruption and formation and induces dental abnormalities in rats. Calcif Tissue Int 2010;86(6):502-510.
• Bradaschia-Correa V, Massa LF, Arana-Chavez VE. Effects of alendronate on tooth eruption and molar root formation in young growing rats. Cell Tissue Res 2007;330(3):475-485.
• Rogers MJ, Gordon S, Benford HL, Coxon FP, Luckman SP, Monkkonen J, Frith JC. Cellular and molecular mechanisms of action of bisphosphonates. Cancer 2000;88(12 Suppl):2961-2978.
• Vasikaran SD. Bisphosphonates: An overview with special reference to alendronate. Ann Clin Biochem 2001;38(Pt 6):608-623.
• Bachrach LK, Ward LM. Clinical review 1: Bisphosphonate use in childhood osteoporosis. J Clin Endocrinol Metab 2009;94(2):400-409.
• Castillo H, Samson-Fang L, American Academy for Cerebral P, Developmental Medicine Treatment Outcomes Committee Review P. Effects of bisphosphonates in children with osteogenesis imperfecta: An AACPDM systematic review. Dev Med Child Neurol 2009;51(1):17-29.
• Wise GE, King GJ. Mechanisms of tooth eruption and orthodontic tooth movement. J Dent Res 2008;87(5):414-434.
• Chilbule SK, Madhuri V. Complications of pamidronate therapy in paediatric osteoporosis. J Child Orthop 2012;6(1):37-43.
• Piper K, Boyde A, Jones SJ. The effect of 3-amino-1-hydroxypropylidene-1,1-bisphosphonate (APD) on the resorptive function of osteoclasts of known nuclear number. Calcif Tissue Int 1994;54(1):56-61.
• Reitsma PH, Bijvoet OL, Verlinden-Ooms H, van der Wee-Pals LJ. Kinetic studies of bone and mineral metabolism during treatment with (3-amino-1-hydroxypropylidene)-1,1-bisphosphonate (APD) in rats. Calcif Tissue Int 1980;32(1):145-157.
• Grier RL, Wise GE. Inhibition of tooth eruption in the rat by a bisphosphonate. J Dent Res 1998;77(1):8-15.
• Fujita Y, Konoo T, Maki K. Short-term etidronate treatment prevents glucocorticoid-induced bone debility of the mandible in growing rats. Orthod Craniofac Res 2008;11(4):187-195.
• Kamoun-Goldrat A, Ginisty D, Le Merrer M. Effects of bisphosphonates on tooth eruption in children with osteogenesis imperfecta. Eur J Oral Sci 2008;116(3):195-198.
• Fouda N, Caracatsanis M, Kut IA, Hammarstrom L. Mineralization disturbances of the developing rat molar induced by mono- and bisphosphonates. J Biol Buccale 1991;19(1):106-115.
• Bradaschia-Correa V, Barrence FA, Ferreira LB, Massa LF, Arana-Chavez VE. Effect of alendronate on endochondral ossification in mandibular condyles of growing rats. Eur J Histochem 2012;56(2):e24.
• Anil S, Preethanath RS, AlMoharib HS, Kamath KP, Anand PS. Impact of osteoporosis and its treatment on oral health. Am J Med Sci 2013;346(5):396-401.
• Evans KD, Lau ST, Oberbauer AM, Martin RB. Alendronate affects long bone length and growth plate morphology in the oim mouse model for osteogenesis imperfecta. Bone 2003;32(3):268-274.
• Kim MS, Jung SY, Kang JH, Kim HJ, Ko HM, Jung JY, Koh JT, Kim WJ, Kim SM, Lee EJ, Kim SH. Effects of bisphosphonate on the endochondral bone formation of the mandibular condyle. Anat Histol Embryol 2009;38(5):321-326.
• Kimura M, Miyazawa K, Tabuchi M, Maeda H, Kameyama Y, Goto S. Bisphosphonate treatment increases the size of the mandibular condyle and normalizes growth of the mandibular ramus in osteoprotegerin-deficient mice. Calcif Tissue Int 2008;82(2):137-147.