Doğal Zeolitlerin Okratoksin A Adsorbsiyon Kapasitelerinin Geliştirilmesi ve Üzüm Suyundan Okratoksin A Uzaklaştırma İşleminde Kullanılması

Year 2020, Volume: 10 Issue: 2, 360 - 379, 15.12.2020

Levent Şen

https://doi.org/10.31466/kfbd.807995

Abstract

Okratoksin A (OTA), üzüm ve üzüm ürünlerinde en sık rastlanan mikotoksindir. Zeolitlerin gıda ve yemlerde bulunan mikotoksinlerin detoksifikasyonlarında kullanımı son yıllarda önemli bir araştırma konusu haline gelmiştir. Çalışmada, iki farklı boyutta (100 μm ve 1-3 mm) doğal zeolit (DZ), OTA adsorbsiyon kapasitesinin artırılması amacıyla Oktadesil trimetil amonyum bromid (ODTMA) ile muamele edilerek modifiye organo-zeolit (MZ) sentezi gerçekleştirilmiştir. Sentezlenen MZ’lerin OTA adsorbsiyon kapasiteleri DZ’ler ile birlikte değerlendirilmiştir. Bu amaçla pH’ı 3’e ayarlanmış OTA içeren tampon çözeltilere ilave edilen DZ ve MZ’lerin maksimum adsorbsiyon gerçekleştirdiği miktar, süre ve sıcaklıklar belirlenerek, üzüm suyundan OTA adsorbsiyonu için optimum koşullar belirlenmiştir. Gerek tampon gerekse de üzüm suyu denemelerinde MZ’ler DZ’lere göre daha iyi adsorbsiyon sonuçları vermişlerdir. 10 ng.mL-1 OTA içeren üzüm suyu, 5% oranında ilave edilen DZ ve MZ’ler eşliğinde 45 °C sıcaklıkta 120 dakika karıştırma süresine tabi tutulduğunda en yüksek adsorbsiyon oranı 89.68%±1.48 ile MT örneğinde gerçekleşmiştir. 1-3 mm boyutundaki zeolit tipleri tüm denemelerde toz formunda olanlara göre daha düşük adsorbsiyon yeteneği göstermiştir. DZ’lerin yüzey yapısının modifiye edilerek OTA adsorbsiyon kapasitesinin artırılması ileüzüm suyundan OTA uzaklaştırılmasında aktif karbonlara alternatif olarak kullanılabileceği düşünülmektedir.

Keywords

Okratoksin A, Zeolit, Adsorbsiyon, Organo-zeolit

Supporting Institution

Giresun Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FEN-BAP-A-140316-35

Improvement of Ochratoxin A Adsorption Capacity of Natural Zeolites and Its Use in the Removal of Ochratoxin A from Grape Juice

Abstract

Octratoxin A (OTA) is the most common mycotoxin in grape and grape products. The use of zeolites in detoxification of mycotoxins in food and feed has become an important research topic in recent years. In this study, Modified organo-zeolite (MZ) synthesis was carried out by treating natural zeolite (DZ) in two different sizes (100 μm and 1-3 mm) with octadecyl trimethyl ammonium bromide (ODTMA) in order to raise the OTA adsorption capacity. OTA adsorption capacities of synthesized MZs were evaluated together with DZ. For this purpose, optimum conditions for OTA adsorption from grape juice were determined by determining the amount, time and temperature at which DZ and MZ added to buffer solutions containing OTA adjusted to pH 3 were subjected to maximum adsorption. In both buffer and grape juice trials, MZs gave better adsorption results than DZs. Grape juice containing 10 ng.mL-1 OTA was subjected to stirring for 120 min at 45 ° C in the presence of DZ and MZ added at 5%, resulting in the highest adsorption rate of 89.68% ± 1.48 for MT added samples. Zeolite types 1-3 mm in size showed lower adsorption ability than those in powder form in all experiments. It is thought that the surface structure of DZs can be modified to increase the OTA adsorption capacity and be used as an alternative to activated carbons in the removal of OTA from grape juice.

Keywords

Octratoxin A, Zeolite, Adsorbtion, Organozeolite

Project Number

FEN-BAP-A-140316-35

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