Cyprinus carpio Karaciğer Dokusu Yağ Asidi İçeriğinin Mevsimsel Değişimi
Year 2022,
Volume: 12 Issue: 1, 66 - 81, 15.06.2022
Semra Kaçar
,
Mehmet Başhan
Abstract
Bu çalışmada, dişi ve erkek C. carpio’nun karaciğer dokusu yağ asidi bileşiminin mevsime bağlı değişimleri araştırılmıştır. Balıkların kas dokusu total lipit yağ asidi kompozisyonları; yağ asidi standartları kullanılarak tespit edilmiştir. Tüm mevsimlerde C. carpio’nun karaciğer dokusundaki total lipitlerde SFA (doymuş yağ asitleri) içinde temel bileşen 16:0, MUFA (tekli doymamış yağ asitleri) içinde 16:1n-7 ve 18:1n-9, PUFA (çoklu doymamış yağ asitleri) içinde EPA ve DHA olmuştur. Balığın karaciğer dokusundaki TAG (triaçilgliserol) ve PL (fosfolipid) yağ asidi içeriklerinin farklı olduğu bulunmuştur. Balıkların karaciğer dokusunda baskın yağ asitleri; üreme, sıcaklık ve mevsime bağlı olarak değişmiştir. C. carpio’nun her iki eşeyinin total lipitlerinde n-3/n-6 oranı; karaciğer dokusunda 1.33-2.55 aralığında tespit edilmiştir.
Supporting Institution
DÜBAP tarafından desteklenmiştir
Project Number
Proje No: 08-FF07
References
- Ackman, R. G., Mcleod, C., Rakshit, S., and Mısra, K. K., (2002). Lipids and fatty acids of five freshwater food fishes of India. Journal of Food Lipids, 9 (2), 127-145.
- Agren, J., Mute, P., Hanninen, O., Herranen, J., and Penttila, I., (1987). Seasonal variation of lipid fatty acids of Boreal freshwater fish species. Comparative Biochemistry and Physiology, 88 B, 905-909.
- Akpınar, M.A., (1986). Cyprinus carpio L. (Osteichthyes:Cyprinidae)’nın karaciğer yağ asitlerinin mevsimsel değişimi. Doğa TU Biyoloji, 10(3),232-239.
- Aras, N. M., Haliloğlu, H. I., Ayık, Ö., and Yetim, H., (2003). Comparison of fatty acid profiles of different tissues of mature trout (Salmo trutta labrax, Pallas, 1811) caught from Kazandere Creek in the Çoruh Region, Erzurum, Turkey. Turkish Journal of Veterinary and Animal Science, 27, 311-316.
- Cejas, J.R., Almansa, E., Villamandos, J.E., Badia, P., Bolanos, A., and Lorenzo, A., (2003). Lipid and fatty acid composition of ovaries from wild fish and ovaries and eggs from captive fish of white sea bream (Diplodus sargus). Aquaculture, 216(1-4), 299-313.
- Crowford, R. H., Cusack, R. R., and Parlee, T. R., (1986). Lipid content and energy expenditure in the spawning migration of alewife (Alosa pseudoharengus) and bluelback herring (Alosa aestivalis). Canadian Journal of Zoology, 64, 1902-1907.
- Folch, J., Lees, M., and Sladane-Stanley, G. H. A., (1957). Simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry, 226, 497-509.
- Halver, J. E., (1989). Fish Nutrition. Academic Press, Inc. 2nd Edition, p.798. New York.
- Hazel, J. R., (1979). The influence of temperature adaptation on the composition of the neutral lipid fraction of rainbow trout (Salmo gairdneri) liver. Journal of Experimental Zoology, 207, 33-41.
- Henderson, R.J, and Tocher, D.R., (1987). The lipid composition and biochemistry of freshwater fish. Progress in Lipid Research, 26, 281–347.
- Jacquot, R. 1961. Organic constituents of fish and other aquatic animals. Fish as food. Borgstrom. G. (Ed.). Academic Press, p. 145-209. New York and London.
- Johansson, L., Kiessling, A., Kiessling, K. H., and Berglund, L., (2000). Effects of altered ration levels on sensory characteristics, lipid content and fatty acid composition of rainbow trout (Oncorhynchus mykiss) their intrinsic variation and practical implications. Food Quality and Preference, 11 (3), 247-254.
- Kaçar, S., and Başhan, M., (2015). Seasonal variations on the fatty acid composition of phospholipid and triacylglycerol in gonad and liver of Mastacembelus simack. Journal of the American Oil Chemists' Society, 92 (9), 1313-1320.
- Kaçar, S., and Başhan, M., (2017). Variations in the fatty acid compositions of the liver and gonad tissue of spiny eel (Mastacembelus mastacembelus) from Atatürk Dam Lake. Turkish Journal of Biochemistry, 42(6), 617-623.
- Kaçar, S., Kayhan, H., and Başhan, M., (2021). Seasonal effects of the fatty acid composition of phospholipid and triacylglycerol in muscle and liver of male Salmo trutta macrostigma. Grasas y Aceites, 72 (4), e435.
- Kaçar, S., and Başhan, M., (2021). Comparative study of lipid and fatty acid profile in liver tissues of male and female Silurus triostegus during the catching seasons. Aquatic Sciences and Engineering, 36(4): 1-9.
- Kaçar, S., and Başhan M. (2021a). Atatürk Baraj Gölü’ndeki Cyprinus carpio (Linnaeus, 1758)’nun kas dokusu yağ asidi içeriğinin mevsimsel değişimi. Fen Bilimleri ve Matematikte Araştırma ve Değerlendirmeler – II. Gece kitaplığı.
- Kaçar, S., and Başhan M. (2021b). Cyprinus carpio gonat dokusu yağ asidi içeriğinin mevsimsel değişimi. Fen Bilimleri ve Matematikte Araştırma ve Değerlendirmeler – I. Gece kitaplığı.
- Karaçalı, M., Bulut, S., Konuk, M., and Solak, K., (2011) Seasonal variations in fatty acid composition of different tissues of mirror carp, Cyprinus Carpio, in Örenler Dam Lake, Afyonkarahisar, Turkey, International Journal of Food Properties, 14:5, 1007-1017.
- Kayhan, H., Başhan, M., and Kaçar, S., (2015). Seasonal variations in the fatty acid composition of phospholipids and triacylglycerols of brown trout. European Journal of Lipid Science and Technology, 116 (5),738-744.
- Kiessling, A., Pickova, J., Johansson, L., Asgard, T., Storebakken, T., and Kiessling, K. H., (2001). Changes in fatty acid composition in muscle and adipose tissue of farmed rainbow trout (Oncorhynchus mykiss) in Relation to Ration and Age. Food Chemistry, 73, 271-284.
- Kminkova, M., Winterova, R., and Kucera, J., (2001). Fatty acids in lipids of carp (Cyprinus carpio) tissues. Czech Journal of Food Science, 19: 177-181.
- Kozlova, T. A., and Khotimchenko, S. V., (2000). Lipids and fatty acids of two pelagic cottoid fishes (Comephorus spp.) endemic to Lake Baikal. Comparative Biochemistry and Physiology, 126 B,477-485.
- Misir, G.B., Tufan, B., and Köse, S., (2016). Variations in total lipid and fatty acid contents of edible muscle, liver, and roes of spotless shad, Alosa Immaculata, during catching season in Black Sea, Journal of Aquatic Food Product Technology, 25(1),2-14.
- Neuhaus, O. W., and Halver, J. C., (1969). Fish in Research, Academic Press. p.135. New York.
- Njinkoué, J-M., Barnathan, G., Miralles, J., Gaydou, E. M., and Samb, A., (2002). Lipids and fatty acids in muscle, liver and skin of three edible fish from the Senegalese coast: Sardinella maderensis, Sardinella aurita and Cephalopholis taeniops. Comparative Biochemistry and Physiology, 131 B, 395-402.
- Rincon-Sanchez, A. R., Hernandez, A., Lopez, M. L., and Mendoza-Figueroa, T., (1992). Synthesis and secretion of lipids by long-term cultures of female rat hepatocytes. Biology of the Cell, 76, 131-138.
- Rodriguez, C., Acosta, C., Badia, P., Cejas, J. R., Santamaria, F. J., and Lorenzo, A., (2004). Assessment of lipid and essential fatty acids requirements of black seabream (Spondyliosoma cantharus) by comparison of lipid composition in muscle and liver of wild and captive adult fish. Comparative Biochemistry and Physiology, 139 B, 619-629.
- Sharma, P., Vikas, K., Sinha, A. K., Jayant, R., Kithsiri, H. M. P., and Gudipati, V., (2009). Comparative fatty acid profiles of wild and farmed tropical freshwater fish rohu (Labeo rohita). Fish Physiology and Biochemistry, 36 (3),411-417.
- Shirai, N., and Wada, S., (2001). Seasonal variation of fatty acid composition of phosphatidylinositol in the dorsal meat, liver and ovary of cultured Japanese catfish Silurus asotus. Fisheries Science, 67,386-388.
- Suzuki, H., Okazaki, K., Hayakawa, S., Wada, S., and Tamura, S., (1986). Influnece of commercial dietary fatty acids on PUFA of cultured freshwater fish and comparison with those of wild fish of the same species. Journal of Agricultural and Food Chemistry, 34, 58-60.
- Steffens, W., and Wirth, M., (1997). Cyprinids as a valuable source of essential fatty acids for human health: A Review. Asian Fisheries Science, 10, 83-90.
- Tufan, B., Koral, S, and Köse, S., (2013). The variations in proximate chemical composition and fatty acid profile in different parts of the thornback ray (Raja clavata) caught from Black Sea, Turkey, Journal of Aquatic Food Product Technology, 22(1),83-95.
- Watanabe, T., (1982). Lipid nutrition in fish. Comparative Biochemistry and Physiology, 73 B, 3-15.
- Yılmaz, Ö., Konar, V., ve Çelik, S., (1995). Elazığ Hazar Gölü’ndeki Capoeta capoeta umbla’nın dişi ve erkek bireylerinde bazı dokularının total lipit ve yağ asidi bileşimleri. Biyokimya Dergisi, 20, 31-42.
Seasonal Variations on the Fatty Acid Composition in Liver Tissue of Cyprinus carpio
Year 2022,
Volume: 12 Issue: 1, 66 - 81, 15.06.2022
Semra Kaçar
,
Mehmet Başhan
Abstract
In this study, seasonal variations on fatty acid composition in liver tissue of female and male C. carpio was investigated. Fatty acid compositions of total lipid have been identified in liver tissues of fishes using a mixture of fatty acid standards. The main constituents were 16:0 among SFAs (saturated fatty acid), 16:1n-7 and 18:1n-9 among MUFAs (monounsaturated fatty acid), EPA and DHA among PUFAs (polyunsaturated fatty acid) in the total lipid extracted from liver tissue of C. carpio all seasons. Triacylglycerol and PL fatty acid contents in muscle tissue of fish were found to be different. The major fatty acids in liver tissue of fish varied depending on reproduction, temperature and season. The n-3/n-6 ratio was determined to range from 1.33-2.55 for liver tissue in total lipids in both sexes of C. carpio.
Project Number
Proje No: 08-FF07
References
- Ackman, R. G., Mcleod, C., Rakshit, S., and Mısra, K. K., (2002). Lipids and fatty acids of five freshwater food fishes of India. Journal of Food Lipids, 9 (2), 127-145.
- Agren, J., Mute, P., Hanninen, O., Herranen, J., and Penttila, I., (1987). Seasonal variation of lipid fatty acids of Boreal freshwater fish species. Comparative Biochemistry and Physiology, 88 B, 905-909.
- Akpınar, M.A., (1986). Cyprinus carpio L. (Osteichthyes:Cyprinidae)’nın karaciğer yağ asitlerinin mevsimsel değişimi. Doğa TU Biyoloji, 10(3),232-239.
- Aras, N. M., Haliloğlu, H. I., Ayık, Ö., and Yetim, H., (2003). Comparison of fatty acid profiles of different tissues of mature trout (Salmo trutta labrax, Pallas, 1811) caught from Kazandere Creek in the Çoruh Region, Erzurum, Turkey. Turkish Journal of Veterinary and Animal Science, 27, 311-316.
- Cejas, J.R., Almansa, E., Villamandos, J.E., Badia, P., Bolanos, A., and Lorenzo, A., (2003). Lipid and fatty acid composition of ovaries from wild fish and ovaries and eggs from captive fish of white sea bream (Diplodus sargus). Aquaculture, 216(1-4), 299-313.
- Crowford, R. H., Cusack, R. R., and Parlee, T. R., (1986). Lipid content and energy expenditure in the spawning migration of alewife (Alosa pseudoharengus) and bluelback herring (Alosa aestivalis). Canadian Journal of Zoology, 64, 1902-1907.
- Folch, J., Lees, M., and Sladane-Stanley, G. H. A., (1957). Simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry, 226, 497-509.
- Halver, J. E., (1989). Fish Nutrition. Academic Press, Inc. 2nd Edition, p.798. New York.
- Hazel, J. R., (1979). The influence of temperature adaptation on the composition of the neutral lipid fraction of rainbow trout (Salmo gairdneri) liver. Journal of Experimental Zoology, 207, 33-41.
- Henderson, R.J, and Tocher, D.R., (1987). The lipid composition and biochemistry of freshwater fish. Progress in Lipid Research, 26, 281–347.
- Jacquot, R. 1961. Organic constituents of fish and other aquatic animals. Fish as food. Borgstrom. G. (Ed.). Academic Press, p. 145-209. New York and London.
- Johansson, L., Kiessling, A., Kiessling, K. H., and Berglund, L., (2000). Effects of altered ration levels on sensory characteristics, lipid content and fatty acid composition of rainbow trout (Oncorhynchus mykiss) their intrinsic variation and practical implications. Food Quality and Preference, 11 (3), 247-254.
- Kaçar, S., and Başhan, M., (2015). Seasonal variations on the fatty acid composition of phospholipid and triacylglycerol in gonad and liver of Mastacembelus simack. Journal of the American Oil Chemists' Society, 92 (9), 1313-1320.
- Kaçar, S., and Başhan, M., (2017). Variations in the fatty acid compositions of the liver and gonad tissue of spiny eel (Mastacembelus mastacembelus) from Atatürk Dam Lake. Turkish Journal of Biochemistry, 42(6), 617-623.
- Kaçar, S., Kayhan, H., and Başhan, M., (2021). Seasonal effects of the fatty acid composition of phospholipid and triacylglycerol in muscle and liver of male Salmo trutta macrostigma. Grasas y Aceites, 72 (4), e435.
- Kaçar, S., and Başhan, M., (2021). Comparative study of lipid and fatty acid profile in liver tissues of male and female Silurus triostegus during the catching seasons. Aquatic Sciences and Engineering, 36(4): 1-9.
- Kaçar, S., and Başhan M. (2021a). Atatürk Baraj Gölü’ndeki Cyprinus carpio (Linnaeus, 1758)’nun kas dokusu yağ asidi içeriğinin mevsimsel değişimi. Fen Bilimleri ve Matematikte Araştırma ve Değerlendirmeler – II. Gece kitaplığı.
- Kaçar, S., and Başhan M. (2021b). Cyprinus carpio gonat dokusu yağ asidi içeriğinin mevsimsel değişimi. Fen Bilimleri ve Matematikte Araştırma ve Değerlendirmeler – I. Gece kitaplığı.
- Karaçalı, M., Bulut, S., Konuk, M., and Solak, K., (2011) Seasonal variations in fatty acid composition of different tissues of mirror carp, Cyprinus Carpio, in Örenler Dam Lake, Afyonkarahisar, Turkey, International Journal of Food Properties, 14:5, 1007-1017.
- Kayhan, H., Başhan, M., and Kaçar, S., (2015). Seasonal variations in the fatty acid composition of phospholipids and triacylglycerols of brown trout. European Journal of Lipid Science and Technology, 116 (5),738-744.
- Kiessling, A., Pickova, J., Johansson, L., Asgard, T., Storebakken, T., and Kiessling, K. H., (2001). Changes in fatty acid composition in muscle and adipose tissue of farmed rainbow trout (Oncorhynchus mykiss) in Relation to Ration and Age. Food Chemistry, 73, 271-284.
- Kminkova, M., Winterova, R., and Kucera, J., (2001). Fatty acids in lipids of carp (Cyprinus carpio) tissues. Czech Journal of Food Science, 19: 177-181.
- Kozlova, T. A., and Khotimchenko, S. V., (2000). Lipids and fatty acids of two pelagic cottoid fishes (Comephorus spp.) endemic to Lake Baikal. Comparative Biochemistry and Physiology, 126 B,477-485.
- Misir, G.B., Tufan, B., and Köse, S., (2016). Variations in total lipid and fatty acid contents of edible muscle, liver, and roes of spotless shad, Alosa Immaculata, during catching season in Black Sea, Journal of Aquatic Food Product Technology, 25(1),2-14.
- Neuhaus, O. W., and Halver, J. C., (1969). Fish in Research, Academic Press. p.135. New York.
- Njinkoué, J-M., Barnathan, G., Miralles, J., Gaydou, E. M., and Samb, A., (2002). Lipids and fatty acids in muscle, liver and skin of three edible fish from the Senegalese coast: Sardinella maderensis, Sardinella aurita and Cephalopholis taeniops. Comparative Biochemistry and Physiology, 131 B, 395-402.
- Rincon-Sanchez, A. R., Hernandez, A., Lopez, M. L., and Mendoza-Figueroa, T., (1992). Synthesis and secretion of lipids by long-term cultures of female rat hepatocytes. Biology of the Cell, 76, 131-138.
- Rodriguez, C., Acosta, C., Badia, P., Cejas, J. R., Santamaria, F. J., and Lorenzo, A., (2004). Assessment of lipid and essential fatty acids requirements of black seabream (Spondyliosoma cantharus) by comparison of lipid composition in muscle and liver of wild and captive adult fish. Comparative Biochemistry and Physiology, 139 B, 619-629.
- Sharma, P., Vikas, K., Sinha, A. K., Jayant, R., Kithsiri, H. M. P., and Gudipati, V., (2009). Comparative fatty acid profiles of wild and farmed tropical freshwater fish rohu (Labeo rohita). Fish Physiology and Biochemistry, 36 (3),411-417.
- Shirai, N., and Wada, S., (2001). Seasonal variation of fatty acid composition of phosphatidylinositol in the dorsal meat, liver and ovary of cultured Japanese catfish Silurus asotus. Fisheries Science, 67,386-388.
- Suzuki, H., Okazaki, K., Hayakawa, S., Wada, S., and Tamura, S., (1986). Influnece of commercial dietary fatty acids on PUFA of cultured freshwater fish and comparison with those of wild fish of the same species. Journal of Agricultural and Food Chemistry, 34, 58-60.
- Steffens, W., and Wirth, M., (1997). Cyprinids as a valuable source of essential fatty acids for human health: A Review. Asian Fisheries Science, 10, 83-90.
- Tufan, B., Koral, S, and Köse, S., (2013). The variations in proximate chemical composition and fatty acid profile in different parts of the thornback ray (Raja clavata) caught from Black Sea, Turkey, Journal of Aquatic Food Product Technology, 22(1),83-95.
- Watanabe, T., (1982). Lipid nutrition in fish. Comparative Biochemistry and Physiology, 73 B, 3-15.
- Yılmaz, Ö., Konar, V., ve Çelik, S., (1995). Elazığ Hazar Gölü’ndeki Capoeta capoeta umbla’nın dişi ve erkek bireylerinde bazı dokularının total lipit ve yağ asidi bileşimleri. Biyokimya Dergisi, 20, 31-42.