The removal of foodborne pathogen biofilms with the treatment of ultrasound and/or organic acid

Year 2022, Volume: 12 Issue: 2, 905 - 915, 15.12.2022

Emel Ünal Turhan , Süleyman Polat

https://doi.org/10.31466/kfbd.1173998

Abstract

The purpose of this study is to investigate the effect of ultrasound and/or organic acids against Escherichia coli and Listeria monocytogenes biofilms on microplates. In the inactivation trials, pathogen biofilms formed on microplates were exposed to 2% organic acid (acetic, citric, malic and lactic acid) and/or ultrasound for 10, 30 and 60 minutes. Biofilm detachment effect of treatment with US and/or organic acid was tested by microplate method based on measuring of optical density. In this study, the removal of foodborne pathogen biofilms varied depending on the treatment method (single or combined), the treatment time and the type of organic acid (P<0.05). The combined treatment with organic acid and ultrasound created additional biofilm detachment. 60 min ultrasound treatment with organic acids caused the highest removal of E. coli (77%) and L. monocytogenes (70%) biofilms. Lactic acid and malic acid among organic acids were the most effective against both of pathogen biofilms on microplates. With the increasing treatment time, the greater biofilm detachment was observed on microplates. The combination treatment of organic acid and ultrasound ensured safe and more effective decontamination against pathogen biofilms on microplates according to single treatment. These findings indicate that ultrasound treatment combined with organic acids can successfully be applied as an environmentally friendly biofilm detachment technique in food industry.

Keywords

Biofilm, Escherichia coli, Listeria monocytogenes, Organic acid, Ultrasound

Supporting Institution

TÜBİTAK

Project Number

118O731

Thanks

This research was supported by the Scientific and Technical Research Council of Turkey (TUBITAK, Project Number: 118O731) and Research Council of Osmaniye Korkut Ata University (Project Number: OKÜBAP-2019-PT2-002).

Ultrason ve/veya organik asit muamelesiyle gıda kaynaklı patojen biyofilmlerin uzaklaştırılması

Abstract

Bu çalışmanın amacı, mikroplaklardaki Escherichia coli ve Listeria monocytogenes biyofilmlerine karşı ultrason ve/veya organik asitlerin etkisini araştırmaktır. İnaktivasyon denemelerinde, mikroplaklarda oluşturulan patojen biyofilmler 10, 30 ve 60 dakika boyunca %2 oranında organik asit (asetik asit, sitrik asit, malik asit ve laktik asit) ve/veya ultrasona maruz bırakılmıştır. Ultrason ve/veya organik asitle muamelenin biyofilm ayırma etkisi optik yoğunluk ölçümüne dayalı mikroplak yöntemiyle test edilmiştir. Bu çalışmada, gıda kaynaklı biyofilmlerin uzaklaştırılması muamele yöntemine (tek veya kombine), muamele süresine ve organik asit türüne bağlı olarak değişmiştir (P<0.05). Organik asit ve ultrason ile kombine muamele daha fazla biyofilm ayrılması yaratmıştır. 60 dakika organik asitli ultrason muamelesi en yüksek E. coli (%77) ve L. monocytogenes (%70) uzaklaştırmasına neden olmuştur. Organik asitler arasında laktik asit ve malik asit, mikroplaklar üzerindeki patojen biyofilmlerin her ikisine karşı da en etkilidir. Artan muamele süresiyle mikroplaklar üzerinde daha çok biyofilm ayrılması gözlenmiştir. Organik asit ve ultrason kombinasyon muamelesi, tekli muameleye göre mikroplaklardaki patojen biyofilmlere karşı daha güvenli ve etkili dekontaminasyon sağlamıştır. Bu bulgular organik asitlerle kombine edilen ultrason muamelesinin gıda endüstrisinde çevre dostu bir biyofilm ayırma tekniği olarak başarılı bir şekilde uygulanabileceğini gösterir.

Keywords

Biyofilm, Escherichia coli, Listeria monocytogenes, Organik asit, Ultrason

Project Number

118O731

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