Arpada Hücresel Apoptoz Hassasiyet (CAS) Geninin Susturularak Külleme Hastalığına Karşı Dirençte Etkisinin Belirlenmesi

Year 2018, Volume: 13 Issue: 1, 40 - 51, 12.10.2018

Figen Ersoy

Abstract

Ekonomik olarak önemli
bir tahıl bitkisi olan arpa, dünyada ekim alanı açısından buğday, çeltik ve
mısırdan sonra gelmektedir. Blumeria graminis f. sp. hordei (Bgh)
tarafından oluşturulan külleme hastalığı arpanın en önemli hastalıklarından
biridir. Hücresel Apoptoz Hassasiyet (Cellular apoptosis susceptiblity; CAS) geninin, arpada külleme hastalığı
sırasında ekspresyonunun arttığı tespit edilmiştir. R geni tarafından
yönetilen sinyal iletim mekanizmaları hakkında kısıtlı bilgi bulunduğu için,
CAS proteininin külleme direnci veya hassasiyeti sırasındaki fonksiyonu
anlaşılmaya çalışılmıştır. CAS proteininin patojene karşı oluşan tepki
sırasında rolünü anlamak için Arpa Çizgili Mozaik Virüsü (BSMV) tarafından indüklenen
gen susturma metodu uygulanmıştır. HvCAS geni susturulan bitki
örneklerinde kontrol grubuna (BSMV:00) göre mRNA seviyesi % 54 azalmıştır.
Fakat HvCAS geni susturulan bitki örneklerinde külleme hastalığına karşı
direnç veya hassasiyet tepkisinde önemli bir değişim saptanmamıştır.

Keywords

Arpa, bitki hastalık dirençi, Blumeria graminis f. sp. hordei, külleme, Hücresel Apoptoz Hassasiyeti (CAS), virüs ile uyarılan gen susturma (VIGS)

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