Effect of montelukast on hepatic and renal ischemia/reperfusion injury

Year 2017, , 43 - 51, 18.06.2017

Ali Baran Budak Kemal Korkmaz Hikmet Selçuk Gedik Gökhan Lafçı Ertuğrul Kaya Handan Ankaralı Serdar Günaydın

https://doi.org/10.18663/tjcl.304530

Abstract

Aim: The thoracoabdominal aortic
aneurysm surgery may cause splanchnic ischemia and may result in severe
postoperative complications caused by liver and/or kidney injury. This study
investigated the effect of temporary occlusion of the aorta on the development
of I/R injury of liver and kidney and the protective effect of montelukast, a
selective reverse CysLT1 receptor antagosit.

 Material and Methods: Twenty-one male Sprague-Dawley
rats were randomly assigned to three groups (n=7 per group) as G1 (no aortic
occlusion and montelukast administration), G2 (45 min. aortic occlusion; no
montelukast administration) and G3 (45 min. aortic occlusion, 10 mg/kg montelukast
administration). At the 48^th hour of reperfusion, kidney and liver
samples were dissected for histopathological evaluation and immunohistochemical
staining for HSP70, interleukin-6 and myeloperoxidase (MPO).

 Results: Tissue
samples taken from the kidney showed significant intergroup differences in
terms of tubular cell degeneration, cytoplasmic vacuole formation, hemorrhage
and tubular dilatation (P = 0.001, P = 0.017, P = 0.001, P = 0.001
respectively). There was a significant difference with regards to HSP-70 stain
(P = 0.01) in favor of G1 and G3 with respect to G2. Hepatocyte degeneration in
the liver samples of G3 was significantly lower than that of G1 and G2 (P = 0.027).

 Conclusion: The
findings of the current study demonstrated that montelukast has protective
effects on both kidney and liver; and were confirmed   histopathologically and
immunohistochemically. The protective effects of montelukast can be attributed
to its potential of anti-oxidative and anti-inflammatory actions.

Keywords

Ischemia-reperfusion injury, montelukast, kidney, liver

Montelukastın karaciğer ve böbrekteki iskemi/reperfüzyon hasarına olan etkisi

Abstract

Amaç:
Torakoabdominal aort anevrizması cerrahisinin sebep olabildiği splanknik
iskemi, karaciğer ve/veya böbrek hasarının neden olduğu ciddi postoperatif
komplikasyonlarla sonuçlanabilir. Bu çalışmada seçici tersinir bir CysLT1
reseptör antagonisti olan montelukastın, aortanın geçici oklüzyonuyla yaratılan
böbrek ve karaciğer iskemi-reperfüzyon (I/R) hasarı üzerindeki koruyucu
etkisini incelemek için tasarlandı.

 Gereç ve Yöntemler: Yirmi bir
erkek Sprague-Dawley tipi sıçan, rasgele üç gruba (her grupta n=7) ayrıldı; G1
(aort oklüzyonu ve montelukast uygulaması yok), G2 (45 dakika aort oklüzyonu;
montelukast uygulması yok) ve G3 (45 dakika aort oklüzyonu, 10 mg/kg
montelukast uygulaması). Reperfüzyonun 48. saatinde böbrek ve karaciğerden
alınan doku örneklerinden histopatolojik değerlendirme ve HSP 70, interlökin-6
ve miyeloperoksidaz (MPO) ile immünohistokimyasal boyama yapıldı.

 Bulgular: Böbrekten
alınan doku örnekleri tübüler hücre dejenerasyonu, sitoplazmik vakuol formasyonu,
hemoraji ve tübüler dilatasyon açısındna incelendi ve gruplar arasında
istatistiksel olarak anlamlı fark bulundu (P = 0.001, P = 0.017, P = 0.001). G1
ve G3 gruplarında HSP-70 ile boyanmada G2 ye göre anlamlı fark saptandı (P = 0.001).
G3 karaciğer örneklerinde hepatosit dejenerasyonu G1 ve G2 den anlamlı olarak
düşüktü (P = 0.027).

 Sonuçlar:
Yapılan çalışmanın bulguları, montelukastın böbrek ve karaciğer üzerinde
koruyucu etkileri olduğunu histopatolojik ve immünohistokimyasal olarak
göstermiştir. Montelukastın koruyucu etkileri, ilacın antioksidan ve
antiinflamatuar etkilerine bağlanabilir. 

Keywords

İskemi-reperfüzyon injurisi, montelukast, böbrek, karaciğer

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