Prevention of lung injury by iloprost following hind limb ischemia and reperfuison model in rats

Year 2018, , 76 - 80, 25.06.2018

Atike Tekeli Kunt , Selim İsbir Ali Civelek Serdar Akgun Arzu Ergen Ozlem Yapicier Aydin Sav Sinan Arsan

https://doi.org/10.18663/tjcl.396679

Abstract

Aim: The major part of tissue damage
occurs upon reperfusion and is mediated by activated neutrophils that release
oxygen free radicals. Following hind-limb ischemia/reperfusion, lung injury due
to neutrophil infiltration and oxygen free radicals has been demonstrated.
Previous studies have shown that this injury can be prevented
pharmacologically. Iloprost is a long acting stable analog of prostacyclin. The
aim of this study is to test the effect of iloprost in prevention of lung
injury due to lower limb ischemia/reperfusion.

Material and Methods: Through a midline
laparotomy infrarenal abdominal aorta was approached and cross-clamped in 20
male Spraque-Dawley rats for 2 hours. At the time of declamping Group I animals
(n=8) received iloprost (0,1µg/kg/min) and Group II animals (n=8) received
normal saline (0,1ml/kg/min) continously for 4 hours. Third group was the sham
group (n=4). The lung tissue assays were performed for measurement of lipid
peroxidation end product malondealdehyde and also total glutathione. Lung
tissues were also examined histopathologically under light microscopy.

Results: The malondealdehyde levels in
the iloprost group were significantly lower than the control group (p<0,05).
The glutathione levels did not show any difference between the iloprost and the
control groups (p>0,05). Histopathological examination revealed that the
structure of the lung tissue was preserved in the iloprost group whereas lung
tissue of the control group had evidence of injury.

Conclusion: Our results suggest that
iloprost reduces the production of oxygen free radicals and prevents lung
injury due to lower limb ischemia reperfusion.

Keywords

ischemia, reperfusion, iloprost

Sıçanlarda alt ekstremite iskemi reperfüzyon hasarını takiben gelişen akciğer hasarının iloprost ile önlenmesi

Abstract

Amaç:  İskemi ve bunu takip
eden reperfüzyon dönemi sonrasında ortaya çıkan doku hasarı

büyük ölçüde reperfüzyon sırasında
gerçekleşir. Aktive olmuş nötrofillerden salınan serbest

oksijen radikalleri bu hasarda
önemli rol oynarlar. Alt ekstremite iskemi-reperfüzyonu

sonrasında görülen akciğer
hasarında nötrofil infiltrasyonunun ve serbest oksijen

radikallerinin önemi
gösterilmiştir. Bu hasarı farmakolojik olarak önlemek için birçok çalışma

yapılmıştır. İloprost, uzun etkili
bir prostasiklin analoğudur. Çalışmanın amacı, alt ekstremite

iskemi reperfüzyonu sonrası ortaya
çıkan akciğer hasarını önlemedeki iloprostun etkisini

araştırmaktır.

Gereç ve Yöntemler: Çalışmada 20 adet Spraque-Dawley cinsi erkek
sıçan kullanıldı. Orta hattan

yapılan laparotomi ile abdominal aortaya
ulaşıldı. Grup I (n=8) ve Grup II (n=8) de bulunan

deneklerin infrarenal aortalarına 2
saat boyunca kross klemp uygulandı. Klemp kaldırıldığı

sırada Grup I’de bulunan deneklere
0,1 g/kg/dk dozunda iloprost ve Grup II’de bulunan

deneklere 0,1ml/kg/dk serum
fizyolojik sürekli infüzyon şeklinde 4 saat boyunca verildi. Grup

III(n=4) sham grubu olarak
belirlendi. Denekler 4. saatin sonunda sakrifiye edilerek akciğer

dokuları çıkarıldıve biyokimyasal
ve histopatolojik inceleme yapıldı.

Bulgular: İloprost verilen grupta malondealdehyde seviyeleri
kontrol grubuna göre

istatistiksel olarak anlamlı
derecede düşük bulundu (p<0.05). Glutatyon düzeylerinde ise

kontrol ve iloprost grupları
arasında anlamlı fark bulunmadı (p>0.05). Histopatolojik

incelemede, iloprost verilen grupta
akciğer dokusunun korunmuş olduğu gözlenirken kontrol

grubunda akciğer doku harabiyeti
tespit edilmiştir.

Sonuç: Sonuçlarımız alt ekstremite iskemi-reperfüzyonu sonrasında
ortaya çıkan akciğer

hasarının önlenmesinde iloprostun
serbest oksijen radikal oluşumunu azaltarak etkili

olduğunu göstermektedir.

Keywords

iloprost, iskemi, reperfüzyon, iloprost

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