FARKLI ÇİÇEK BALLARININ ANTİMİKROBİYAL AKTİVİTELERİNİN BELİRLENMESİ

Year 2020, Volume: 20 Issue: 1, 38 - 50, 12.06.2020

Aycan Çınar

https://doi.org/10.31467/uluaricilik.687207

Cited By: 2

Abstract

Bal, besleyici özelliği yüksek, biyoaktif bileşence zengin fonksiyonel bir gıdadır. Balın biyolojik aktivitesinin botanik orijin, coğrafya ve iklim özelliklerine göre farklılık göstermesi, ülkemizde üretilen monofloral ve multifloral balların kapsamlı olarak ele alınmasını gerekli kılmaktadır. Doğal koruyucuların sentetik ürünlerle yer değiştirme anlayışının benimsenmesi, balın alternatif kullanım olanaklarının araştırılmasını sağlamaktadır. Bu amaçla, çalışmamızda lavanta, limon çiçeği, kekik ve multifloral balların antimikrobiyal aktivitesi belirlenmiş ve antimikrobiyal etkinlikleri karşılaştırılmıştır. Elde edilen sonuçlara göre, genel olarak multifloral balın monofloral ballardan daha yüksek antimikrobiyal aktiviteye sahip olduğu, monofloral ballardan limon çiçeği balının test edilen mikroorganizmalara karşı güçlü inhibisyon gösterdiği, kekik balının ise en zayıf antibakteriyel etkiye sahip olduğu saptanmıştır. Tüm bal çeşitlerinde antimikrobiyal aktivitenin (Bacillus cereus DSM 4312 hariç) sırasıyla bakteri > maya> küf olduğu tespit edilmiştir. Bununla birlikte, istatistiksel olarak en dirençli bakterinin B. cereus DSM 4312, en duyarlı bakterilerin ise Klebsiella pneumoniae ATCC 700603 ve Pseudomonas aeruginosa ATCC 35032 olduğunu söylemek mümkündür. 

Keywords

bal, antibakteriyel aktivite, antifungal aktivite, monofloral, multifloral

DETERMINATION OF ANTIMICROBIAL ACTIVITIES OF DIFFERENT FLOWER HONEYS

Abstract

Honey is highly nutritious, and a functional food rich in bioactive components. The biological activity of honey differs according to its botanical origin, geographic properties, and climate characteristics. Therefore, it is necessary to handle the monofloral and multifloral honey produced in our country in a comprehensive manner. Adopting the understanding of displacement of natural preservatives with synthetic ones enables the exploration of alternative uses of honey. For this purpose, in our study, the antimicrobial activity of lavender, lemon, thyme and multifloral honey were determined and compared with each other. According to the results obtained, it was found that multifloral honey has higher antimicrobial activity than monofloral honey, but lemon honey which is one of the monofloral honey types, shows strong inhibition against microorganisms tested, and thyme honey had the weakest antibacterial effect. Antimicrobial activity (except for Bacillus cereus DSM 4312) was found to be strongest against bacterial then yeast and then mold in all honey varieties. In addition, we found that that the most resistant bacteria statistically was B. cereus DSM 4312, while the most sensitive bacteria was Klebsiella pneumoniae ATCC 700603 and Pseudomonas aeruginosa ATCC 35032.

Keywords

Bal, Antibakteriyel Aktivite, Antifungal Aktivite, Monofloral, Multifloral

References

• Alandejani, T., Marsan, J., Ferris, W., Slinger, R., Chan, F. 2009. Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilms Otolaryngol. Head Neck Surg. 141(1): 114–118, doi.org/10.1016/j.otohns.2009.01.005
• Ali, SAM., Mahdi, WM., Altai, SH. 2018. Antibacterial Activities of Natural Iraqi and Commercial Honey Against Klebsiella pneumoniae Journal of Tikrit University For Agriculture Sciences. 18(4): 164-169.
• Allen, KL., Molan, PC., Reid, GM. 1991. A survey of the antibacterial activity of some New Zealand honeys Journal of Pharmacy and Pharmacology. 43(12): 817-822., doi.org/10.1111/j.2042-7158.1991.tb03186.x
• Alvarez-Suarez, JM., Tulipani, S., Diaz, S., Estevez, Y., Romandini, S., Giampieri, F., Damiani, P., Astolfi, S., Bompadre, S., Batting, M. 2010. Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with colour, polyphenol content and others chemical compounds Food and Chemical Toxicol. 48: 2490- 2499., doi.org/10.1016/j.fct.2010.06.021
• Alzahrani, HA., Alsabehi, R., Boukraâ, L., Abdellah, F., Bellik, Y., Bakhotmah, BA. 2012. Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins Molecules. 17(9): 10540-10549., doi.org/10.3390/molecules170910540
• Anand, S., Deighton, M., Livanos, G., Morrison, PD., Pang, ECK., Mantri, N. 2019. Antimicrobial activity of Agastache Honey and characterization of its bioactive compounds in comparison with important commercial honeys Frontiers in Microbiology. 10: 269-285., doi.org/10.3389/fmicb.2019.00263
• Anthimidou, E., Mossialos, D. 2013. Antibacterial activity of Greek and Cypriot honeys against Staphylococcus aureus and Pseudomonas aeruginosa in comparison to manuka honey Journal of medicinal food. 16(1): 42-47., doi.org/10.1089/jmf.2012.0042
• Asadi-Pooya, AA., Pnjehshahin, MR., Beheshti, S. 2003.The antimycobacterial effect of honey:an in vitro study Riv. Biol. 96(3), 491- 495.
• Ávila, S., Hornung, PS., Teixeira, GL., Malunga, LN., Apea-Bah, FB., Beux, MR., Beta, T., Ribani, RH. 2019. Bioactive compounds and biological properties of Brazilian stingless bee honey have a strong relationship with the pollen floral origin Food Research International 123: 1–10., doi.org/10.1016/j.foodres.2019.01.068
• Aziz, MSA., Giribabu, N., Rao, PV., Salleh, N. 2017. Pancreatoprotective effects of Geniotrigona thoracica stingless bee honey in streptozotocin-nicotinamide-induced male diabetic rats Biomedicine & Pharmacotherapy. 89: 135–145., doi.org/10.1016/j.biopha.2017.02.026
• Balouiri, M., Sadiki, M., Ibnsouda, SK. 2016. Methods for in vitro evaluating antimicrobial activity: A review Journal of pharmaceutical analysis. 6(2): 71-79., doi.org/10.1016/j.jpha.2015.11.005
• Bansal, V., Medhi, B., Pandhi, P. 2005. Honey–a remedy rediscovered and its therapeutic utility Kathmandu Univ. Med. J. 3(3): 305–309.
• Benlyas, M., Alem, C., Filali-Zegzouti, Y. 2016. Evaluation of antioxidant, antibacterial and antifungal activities of eleven monofloral honey samples collected from Morocco Journal of Chemical and Pharmaceutical Research. 8(3): 299-306.
• Bogdanov, S. 1997. Nature and origin of the antibacterial substances in honey. LWT-Food Science and Technology. 30(7): 748-753., doi.org/10.1006/fstl.1997.0259
• Bogdanov, S., Ruoff, K., Persano, Oddo L. 2004. Physico-chemical methods for the characterisation of unifloral honeys: A review Apidologie, 35(1): 4-17., doi.org/10.1051/apido:2004047
• Borsato, DM., Prudente, AS., Döll-Boscardin, PM., Borsato, AV., Luz, CFP., Maia, BHLNS., Miguel, OG. 2014. Topical anti-Inflammatory activity of a monofloral honey of Mimosa scabrella provided by Melipona marginata during winter in Southern Brazil Journal of Medicinal Food. 17(7): 817–825., doi.org/10.1089/jmf.2013.0024
• Candiracci, M., Citterio, B., Piatti E. 2012. Antifungal activity of the honey flavonoid extract against Candida albicans Food Chem.131(2): 493 - 499., doi.org/10.1016/j.foodchem.2011.09.012
• CLSI. 2015. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard—Tenth Edition. CLSI document M07-A10. Wayne, PA.
• Cohen, HA., Rozen, J., Kristal, H., Laks, Y., Berkovitch, M., Uziel, Y., Kozer, E., Pomeranz A., Efrat H. 2012. Effect of Honey on Nocturnal Cough and Sleep Quality: A Double-blind, Randomized, Placebo-Controlled Study Pediatrics. 130(3): 465-471. doi.org/10.1542/peds.2011-3075
• El-Gendy, MMA. 2010. In vitro evaluation of medicinal activityof Egyptian honey from different floral sources as anticancer and antimycotic infective agents J. Microbiol. Biochem. Technol. 2, 118–123., doi.org/10.4172/1948-5948.1000035
• Erejuwa, OO., Sulaiman, SA., Wahab, MS. 2014. Effects of honey and its mechanisms of action on the development and progression of cancer Molecules.19(2):2497-522., doi.org/10.3390/molecules19022497
• Ertürk, Ö., Şahin, H., Kolaylı, S., Ayvaz, M. Ç. 2014. Antioxidant and antimicrobial activity of East Black Sea Region honeys Turkish Journal of Biochemistry/Turk Biyokimya Dergisi. 39(1): 99-106.
• Estevinho, ML., Afonso, SE., Feás, X. 2011. Antifungal effect of lavender honey against Candida albicans, Candida krusei and Cryptococcus neoformans Journal of food science and technology. 48(5): 640-643.
• Eteraf-Oskouei, T., Najafi, M. 2013. Traditional and modern uses of natural honey in human diseases: A review Iran. J. Basic Med. Sci. 16, 731–742. EUCAST. 2015. Method for susceptibility testing of moulds; For the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia forming moulds. Copenhagen, Denmark
• Fauzi, AN., Norazmi, MN., Yaacob, NS. 2011. Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines Food. Chem. Toxicol. 49(4):871-878., doi.org/10.1016/j.fct.2010.12.010
• Feás, X., Estevinho, LM. 2011. A survey of the in vitro antifungal activity of heather (Erica sp.) organic honey J Med Food.14(10), 1284–1288., doi.org/10.1089/jmf.2010.0211
• Gallardo-Chacón, JJ., Caselles, M., Izquierdo-Pulido, M., Rius, N. 2008. Inhibitory activity of monofloral and multifloral honeys against bacterial pathogens Journal of apicultural research. 47(2): 131-136., doi.org/10.1080/00218839.2008.11101439
• Gheldof, N., Wang, XH., Engeseth, NJ. 2003. Buckwheat honey increases serum antioxidant capacity in humans J. Agric. Food Chem. 51(5):1500-1505., doi.org/10.1021/jf025897t
• Irish, J., Carter, D. A., Shokohi, T., Blair, SE. 2006. Honey has an antifungal effect against Candida species. Medical Mycology. 44(3): 289-291., doi.org/10.1080/13693780500417037
• Jaganathan, SK., Mandal, M. 2009. Honey constituents and their apoptotic effect in colon cancer cells J. ApiProduct ApiMedical Sci. 1(2): 29–36.
• Kacaniova, M., Fatrcova-Sramkova, K., Nozkova, J., Melich, M., Kadasi-Horakova, M., Knazovicka, V., Mariassyova, M. 2010. Antiradical activity of natural honeys and antifungal effect against Penicillium genera Journal of Environmental Science and Health Part B. 46(1): 92-96., doi.org/10.1080/03601234.2011.534416
• Kadar, M., Juan‐Borrás, M., Carot, J. M., Domenech, E., Escriche, I. 2011. Volatile fraction composition and physicochemical parameters as tools for the differentiation of lemon blossom honey and orange blossom honey Journal of the Science of Food and Agriculture. 91(15): 2768-2776., doi.org/10.1002/jsfa.4520
• Koc, AN., Silici, S., Ercal, BD., Kasap, F., Hörmet-Öz, HT., Mavus-Buldu, H. 2009. Antifungal activity of Turkish honey against Candida spp. and Trichosporon spp: an in vitro evaluation Sabouraudia, 47(7): 707-712., doi.org/10.3109/13693780802572554
• Kustiawan, PM., Puthong, S., Arung, ET., Chanchao, C. 2014. In vitro cytotoxicity of Indonesian stingless bee products against human cancer cell lines Asian Pacific Journal of Tropical Biomedicine. 4(7): 549–556., doi.org/10.12980/APJTB.4.2014APJTB-2013-0039
• Leyva-Jimenez, FJ., Lozano-Sanchez, J., Borras-Linares, I., Cadiz-Gurrea, ML., Mahmoodi-Khaledi, E. 2018. Potential antimicrobial activity of honey phenolic compounds against Gram positive and Gram negative bacteria LWT- Food Science and Technology, 101: 236-245., doi:10.1016/j.lwt.2018.11.015.
• Magaldi, S., Mata-Essayag, S., De Capriles, CH., Perez, C., Colella, MT., Olaizola, C., Ontiveros, Y. 2004. Well diffusion for antifungal susceptibility testing International Journal of Infectious Diseases. 8(1): 39-45., doi.org/10.1016/j.ijid.2003.03.002
• Mahmoodi-Khaledi, E., Lozano-Sánchez, J., Bakhouche, A., Habibi-Rezaei, M., Sadeghian, I., Segura-Carretero, A. 2017. Physicochemical properties and biological activities of honeys from different geographical and botanical origins in Iran European Food Research and Technology, 243(6): 1019-1030., doi.org/10.1007/s00217-016-2811-0
• Mandal, MD., Mandal, S. 2011. Honey: its medicinal property and antibacterial activity Asian Pacific journal of tropical biomedicine. 1(2): 154-160., doi.org/10.1016/S2221-1691(11)60016-6
• Molan, PC. 1992. The antibacterial activity of honey: 1. The nature of the antibacterial activity Bee World. 73(1): 5-28., doi.org/10.1080/0005772X.1992.11099109
• Molan, PC., Betts, JA. 2004. Clinical usage of honey as a wound dressing: an update J Wound Care. 13(9): 353–6., doi.org/10.12968/jowc.2004.13.9.26708
• NCCLS. 2002. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard—Second Edition. NCCLS document M27-A2 (ISBN 1-56238-469-4). NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA.
• Nolan, VC., Harrison, J., Cox, JAG. 2019. Dissecting the Antimicrobial Composition of Honey Antibiotics. 8(4): 251., doi.org/10.3390/antibiotics8040251
• Onbaşlı, D., Çelik GV., Kahraman, S., Kanbur, M. 2019. Apiterapi ve İnsan Sağlığı Üzerine Etkileri Erciyes Üniv. Vet Fak. Derg. 16(1): 49-56., doi.org/10.32707/ercivet.538001
• Özkök, A., Koru, Ö., Sorkun, K. 2016. Microbiological analysis and antibacterial effects of Turkish thyme honey Bee World. 93(4): 98-101., doi.org/10.1080/0005772X.2016.1275489
• Pérez, RA., Iglesias, MT., Pueyo, E., Gonzalez, M., de Lorenzo, C. 2007. Amino acid composition and antioxidant capacity of Spanish honeys J. Agric. Food Chem. 55(2):360-365., doi.org/10.1021/jf062055b
• Pimentel, RB. de Q., da Costa, CA., Albuquerque, PM., Junior, SD. 2013. Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey BMC Complementary and Alternative Medicine, 13(1): 151., doi.org/10.1186/1472-6882-13-151.
• Ramsay, EI., Rao, S., Madathil, L., Hegde, SK., Baliga-Rao, MP., George, T., Baliga MS. 2019. Honey in oral health and care: A mini review Journal of Oral Biosciences. 61: 32-36., doi.org/10.1016/j.job.2018.12.003
• Rao, PV., Krishnan, KT., Salleh, N., Gan, SH. 2016. Biological and therapeutic effects of honey produced by honey bees and stingless bees: A comparative review Brazilian Journal of Pharmacognosy. 26(5): 657–664., doi.org/10.1016/j.bjp.2016.01.012
• Sagdic, O., Silici, S., Ekici, L. 2013. Evaluation of the phenolic content, antiradical, antioxidant, and antimicrobial activity of different floral sources of honey International Journal of Food Properties. 16(3): 658-666., doi.org/10.1080/10942912.2011.561463
• Samarghandian, S., Farkhondeh, T., Samini, F. 2017. Honey and Health: A Review of Recent Clinical Research Pharmacognosy Research. 9(2): 121-127., doi.org/10.4103/0974-8490.204647
• Saranraj, P., Sivasakthi, S. 2018. Comprehensive Review on Honey: Biochemical and Medicinal Properties J. Acad.Ind. Res, 6, 165–181.
• Seçmen, Ö., Gemici, Y., Görk, G., Bekat, L., Leblebici, E. 2000. Systematic of seeded plants. Bornova, İzmir: Ege Üniversitesi, Faculty of Science.
• Subrahmanyam, M. 1998. A prospective randomised clinical and histological study of superficial burn wound healing with honey and silver sulfadiazine Burns. 24(2): 157–61., doi.org/10.1016/S0305-4179(97)00113-7
• Taormina, PJ., Niemira, BA., Beuchat, LR. 2001. Inhibitory activity of honey against foodborne pathogens as influenced by the presence of hydrogen peroxide and level of antioxidant power International journal of food microbiology. 69(3): 217-225., doi.org/10.1016/S0168-1605(01)00505-0
• Valgas, C., Souza, SMD., Smânia, EF., Smânia JrA. 2007. Screening methods to determine antibacterial activity of natural products Brazilian Journal of Microbiology. 38(2): 369-380., doi.org/10.1590/S1517-83822007000200034
• Voidarou, C., Alexopoulos, A., Plessas, S., Karapanou, A., Mantzourani, I., Stavropoulou, E., Bezirtzoglou, E. 2011. Antibacterial activity of different honeys against pathogenic bacteria Anaerobe. 17(6): 375-379., doi.org/10.1016/j.anaerobe.2011.03.012
• Wang, R., Starkey, M., Hazan, R., Rahme, LG. 2012. Honey’s ability to counter bacterial infections arises from both bactericidal compounds and QS inhibition Front. Microbiol. 3: 144-150., doi.org/10.3389/fmicb.2012.00144
• White Jr, JW., Subers, MH., Schepartz, AI. 1963. The identification of inhibine, the antibacterial factor in honey, as hydrogen peroxide and its origin in a honey glucose-oxidase system. Biochimica et Biophysica Acta (BBA)-Specialized Section on Enzymological Subjects. 73(1): 57-70.
• Wiegand, I., Hilpert, K., Hancock, RE. 2008. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances Nature protocols. 3(2): 163-175.
• Wilkinson, JM., Cavanagh, HM. 2005. Antibacterial activity of 13 honeys against Escherichia coli and Pseudomonas aeruginosa Journal of medicinal food. 8(1): 100-103., doi.org/10.1089/jmf.2005.8.100
• Young, T. 2005. Honey: rediscovering an ancient healer Practice Nursing.16(11): 542–547., doi.org/10.12968/pnur.2005.16.11.19972
• Zafar, H., Israili, MS. 2014. Antimicrobial Properties of Honey American Journal of Therapeutics. 21(4): 304-323., doi.org/10.1097/MJT.0b013e318293b09b