Determination of local expressions of IGF-1, LC3B and NF-kB in white muscle disease in lambs by immunohistochemical method

Year 2024, , 79 - 86, 22.08.2024

Gökhan Akçakavak , Özhan Karataş , Ayşenur Tural , Osman Dağar , Osman Doğan , Mehmet Tuzcu

https://doi.org/10.31797/vetbio.1449118

Abstract

White muscle disease (WMD) is also known as Stiff Lamb Disease or Nutritional Muscular Dystrophy. Selenium and/or Vitamin E deficiency constitutes the etiology of the disease. This study aimed to immunohistochemically evaluate local protein expressions of Nuclear factor kappa B (NF-kB), Insulin-like growth factor-1 (IGF-1) and Microtubule-related protein 1A/1B-light chain 3 beta (LC3B) in WMD. The material of the study consisted of 15 WMD, and 6 healthy lamb heart samples. The heart tissues of the autopsied lambs were subjected to routine tissue processing and paraffin blocks were obtained. Then, it was stained with Hematoxylin-Eosin and immunohistochemical methods. Control group lambs had normal macroscopic appearance. Macroscopically, hyaline degeneration and zenker’s necrosis, calcification areas were observed in WMD tissues. Microscopically, degenerative and necrotic muscle fibers, calcification areas, fibrosis, mononuclear cell infiltrates and macrophage infiltrates were detected in WMD heart tissues. Immunohistochemically, significant increases were detected in IGF-1 (p<0.001), LC3B (p<0.001) and NF-kB (p<0.05) in the WMD group compared to the control group. Immunoreactivity in the relevant primers was detected commonly in degenerative and necrotic muscle fibers. In addition, occasional immunoreactivity was observed in the relevant primers in inflammatory cell infiltrates. In conclusion, NF-kB, IGF-1 and LC3B protein expressions were evaluated immunohistochemically for the first time in lambs with WMD. Our findings show that IGF-1 and LC3B proteins are highly expressed in heart tissue in WMD. Additionally, it is possible to say that IGF-1 and LC3B can be used in the diagnosis of WMD disease.

Keywords

Histopathology, IGF-1, LC3B, NF-kB, white muscle disease

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