Investigation of cytogenetic, electrophoretic, histopathological and biochemical effects of walnut (juglans regia l.) Leaf extract in rats experimentally induced diabetes by streptozotocin

Year 2024, , 96 - 106, 22.08.2024

Pinar Aksu Kılıçle , Evren Koç , Ebru Karadağ Sarı , Abdullah Doğan , Hasan Asker , Yağmur Yıldız Asker

https://doi.org/10.31797/vetbio.1454981

Abstract

In this study, the effects of Juglans regia L. (JR) leaf extract on histological damage and cytogenetic, electrophoretic, and biochemical parameters in the liver and kidney tissues of diabetic rats were investigated. In the study, 60 male rats (Sprague-Dawley) were separated into six groups. According to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) data, it was determined that there were increases and decreases in different serum protein expressions because of the treatment of JR leaf extract in the diabetes group. In the kidney tissue of the diabetes group, an increase in volume in the glomeruli and narrowing of the Bowman's space as well as thickening of the walls of tubules were detected. Vacuolization and shedding were observed in the epithelial cells of tubules in the cortical regions in kidney. In diabetic + JR extract groups administered JR leaf extract at doses of 250 mg/kg and 500 mg/kg, serum AST and ALT levels were reduced compared to the diabetic group. Diabetic rats' livers showed spotted necrosis and fibrosis in the portal area, biliary tract proliferation, mild inflammation, increased vascularization, unicellular necrosis in hepatocytes, and sinusoidal dilatation. The JR leaf extract group did not exhibit these problems. JR leaf extract influences reducing hepatotoxic and oxidative damage due to diabetes and increasing the level of antioxidant enzymes. As a result, it was concluded that the application of JR leaf extract may have a protective effect against damage caused by diabetes.

Keywords

Diabetes, Juglans regia L., kidney, liver, walnut

Ethical Statement

This study was carried out at Kafkas University Research Animals Application Center. This research was approved by The Ethics Committee of the Faculty of Veterinary Medicine, Kafkas University (KAU-HADYEK, Ref No:2020/045 Date: 24.03.2020)

Supporting Institution

Kafkas University

Project Number

2017-FM-80

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