Investigation of the Response of Different Breeds to the Superovulation Treatment

Year 2016, Volume: 1 Issue: 1, 1 - 5, 13.12.2016

Tahir Karaşahin , Sedat Hamdi Kızıl , Muharrem Satılmış , Numan Akyol , Şükrü Dursun , Mesut Kırbaş

Abstract

This
study was carried out to compare three different breeds (Simmental, Holstein
and Brown Swiss) for the response to the superovulation program and embryo
quality. Cows from each breed which have given at least one birth and which are
at the same age and conditions were used. Regardless of the period of the oestrus
cycle, intravaginal CIDR (Pfizer Inc., New York, USA) implant including 1.38 g
progesteron was applied to each cow and kept 9 days long. A total of 400 mg
i.m.  FSH (PLUSET/Laboratorios Calier)
was injected twice a day during 4 days from 7th day after implantation of CIDR
to cows. All animals were injected 150 µg d-choprostenol (Dalmazin/Vetaş)
together with 5th FSH injection, and right after 6th FSH injection CIDR was
removed. After the last FSH injection, in the 12th and 24th hours from the
beginning of oestrus, cows were inseminated. Seven days after first
insemination, embryos were collected via non-surgical uterine flushing.
According to the results, superovulation response was better in Simmental cows
than the others. The best response for superovulation in FSH hormone was also
observed in Simmental breed cows.

Keywords

Superovulation, Embryo, Simmental, Holstein, Brown Swiss

Süperovulasyon Uygulamasına Farklı Irkların Verdikleri Cevapların Araştırılması

Abstract

Bu çalışma, süperovulasyon programı uygulanan 3 farklı sığır ırkının (Simental, Siyah-Alaca ve Esmer) süperovulasyon uygulamasına verdikleri cevapların ve elde edilen embriyo kalitelerinin karşılaştırılması amacıyla yapıldı. Çalışmada her ırktan en az bir doğum yapmış aynı yaş ve kondisyonlara sahip inekler kullanıldı.      Her ineğe östrus siklusunun herhangi bir döneminde 1.38 g progesteron içeren intravaginal CIDR (Pfizer Inc., New York, USA) 9 gün  süreyle  uygulandı. Her ineğe progesteron uygulamasının 7. gününden başlamak üzere günde 2 defa, 4 gün boyunca toplam 400 mg i.m. FSH (PLUSET/Laboratorios Calier) uygulaması yapıldı. Bütün hayvanlara 5. FSH uygulaması ile birlikte 150 µg. d-choprostenol (Dalmazin/Vetaş) yapıldı ve 6. FSH uygulaması ile birlikte CIDR uzaklaştırıldı. Son FSH uygulamasının ardından östrus belirtilerinin 12. ve 24. saatlerinde suni tohumlama yapıldı. İlk suni tohumlamadan 7 (yedi) gün sonra non-surgical yöntemle uterus yıkaması yapılarak embriyolar toplandı. Elde edilen sonuçlara göre Simental ırkı ineklerin süperovulasyon uygulamasına olan cevabının diğer ırklardan daha iyi olduğu gözlendi

Keywords

Süperovulasyon, Embriyo, Simental, Siyah Alaca, Esmer

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