Gökkuşağı Alabalığında Karşılaşılan Bazı Bakteriyel Hastalık Etkenlerinin Hızlı Teşhisi

Year 2018, Volume: 3 Issue: 3, 23 - 27, 31.12.2018

İfakat Tulay Çağatay , Erkan Gümüş

https://doi.org/10.31797/vetbio.425026

Abstract

Bu
çalışmada, PCR yöntemi ile gökkuşağı alabalığında (Oncorhynchus mykiss) hastalığa neden olan beş farklı patojenin (Aeromonas salmonicida, Aeromonas hydophila, Yersinia ruckeri, Flavobacterium psychrophila ve Renibacterium salmoninarum) hızlı teşhis
edilebilmesi için beşyüz elli balık doku (solungaç, böbrek, ağız, deri ve
yüzgeç) örneğinden direk olarak saflaştırılan DNA’lar kullanılmıştır.
Çalışmamızdaki balık hastalık etkenlerini tespit edebilmek için beş farklı
hedef gen bölgesi (gryA, gyrB,
ompTS, n-DNA ve YER) kullanılmıştır ve bunların PCR reaksiyonları
gerçekleştirilmiştir. Çalışmamızda sonuç olarak, hastalıklara neden olan etken
bakteriler, dokulardan izole
edilen DNA’lardaki hedef genlerin PCR methodu kullanılarak tespit
edilmesiyle 3-4 saat içerisinde tanımlanmıştır. 

Keywords

PCR, Balık hastalığı

Rapid Detection of Some Bacterial Diseases in Rainbow Trout

Abstract

In this study, we used five hundred fifty
different tissue samples (gill, kidney, mouth, skin and finn) to detect five
pathogens (A. salmonicida, A. hydophila, Y. ruckeri, F. psychrophila
and R. salmoninarum)
in rainbow trout (Oncorhynchus
mykiss) with using direct PCR methods. Five targeted genes (gryA,
gyrB, ompTS, n-DNA and YER) have been chosen to detect the causative agents of
diseases. As a result, five infection agents of rainbow trout were identified
and determinated within 3-4 hours time by using direct PCR from fish tissues,
without any microbiological examination methods.

Keywords

PCR, Fish Diseases

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