Comparison of graft transplantation into two different regions of freshwater mussel (Unio terminalis)

Year 2020, Volume: 5 Issue: 3, 96 - 105, 31.12.2020

Hülya Şereflişan

https://doi.org/10.31797/vetbio.726763

Abstract

The relationship between the rate of incidence for ketosis seen in the livestock in this study, the best pearl sac formation was investigated in two different implantation areas of the freshwater mussel (Unio terminalis) using two different graft materials. Average dorso-ventral length, height, width and weight of used Unio terminalis mussel were 7.80 ± 0.22 cm, 4.12 ± 0.21 cm, 3.14 ± 0.19 cm and 45.74 ± 0.23 g, respectively. The mantle cavity and intra-gonad regions were determined as pearl pouch formation implantation site and 2 grafts were applied to each region. Mantle tissue slices obtained from the pallial part of the mantle tissue in the size of 3 × 3 mm and the same species mussel shell with 3 mm diameter were used as the graft material. 50 different mussels were used in each application with a total of a total of 300 mussels, three replicated. At the end of the 3-month pearl culture, highest rate of formation (60%) and number (35) of the pearl sac was observed in mantle cavity region compared to intra-gonad region. However, the muscular graft and nucleus removal was detected at the lowest rate (20%) in the region of the gonad which is the best obtained result. This research is important for commercial pearl production studies on the most suitable graft implantation site in the production of cultured pearls.

Keywords

Unio terminalis, pearl pouch, graft, mantle, nacre

Tatlı su midyesinin (Unio terminalis) farklı iki bölgesine yapılan greft naklinin karşılaştırılması

Abstract

Bu çalışmada, tatlı su midyesinin (Unio terminalis) iki farklı implantasyon bölgesinde, iki farklı greft malzemesi kullanılarak en iyi inci kesesi oluşumu incelenmiştir. Araştırmada ortalama dorso-ventral uzunluğu 7.80±0.22 cm, yüksekliği 4.12±0.21 cm, genişliği 3.14±0.19 cm ve ağırlığı 45.74±0.23 g olan Unio terminalis midye türü kullanılmıştır. İnci kese oluşumu için implantasyon bölgesi olarak manto boşluğu ve gonad içi bölgesi belirlenmiş ve her bir bölgeye 2’şer adet greft uygulanmıştır. Greft malzemesi olarak manto dokusunun pallial bölümünden 3×3 mm büyüklüğünde elde edilen manto doku dilimleri ile 3 mm çapında sedefli midye kabuğu kullanılmıştır. Her bir uygulama için 50’şer adet olmak üzere, üç tekerrürlü toplamda 300 adet midye kullanılmıştır. 3 aylık inci kültürü sonunda, manto boşluğu ve gonad içi bölgesinde inci kesesi oluşumu karşılaştırıldığında; nitelikli inci kesesi oluşum oranı (% 60) ve sayısının (35) en iyi olduğu bölgenin manto boşluğu bölgesi olduğu belirlenmiştir. Ancak, midye tarafından greft ve nükleus atımı bakımından gonad içi uygulaması daha başarılı sonuç vermiştir (%20). Bu araştırma, kültür inci üretiminde önemli görülen sedef oluşum süresi ve en uygun greft implantasyon bölgesi konusunda, ticari inci üretim çalışmaları için önem taşımaktadır.

Keywords

Unio terminalis, nacre, inci kesesi, manto, greft

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