Koçlarda spermaya katılan CAPE'in (caffeic acid phenethyl ester) dondurma ve çözdürme sonrası spermatolojik parametreler, oksidatif stres ve DNA hasarı üzerine etkileri

Year 2021, Volume: 6 Issue: 3, 270 - 277, 31.12.2021

Mustafa Gündoğan Deniz Yeni , Fatih Avdatek , Ömer Hazman

https://doi.org/10.31797/vetbio.1008995

Abstract

Bu çalışmanın amacı koç sperma sulandırıcısına ilave edilen farklı dozlardaki caffeic acid phenethyl ester (CAPE) çözüm sonu spermatolojik parametreler, oksidatif stres ve DNA hasarı üzerine etkileri araştırıldı. Ejakulatlar beş baş Merinos koçtan haftada bir kez suni vajen yardımıyla toplandı ve bu işlem altı kere tekrarlandı. Ejakulatlar ml’de 150x106 spermatozoon olacak şekilde antioksidan içermeyen (kontrol) ve antioksidan içeren (10 µg/ml, 50 µg/ml ve 100 µg/ml) sulandırıcılar ile dört bölüme ayrıldı. Sulandırılan örnekler 0,25 ml’lik payetlerde 5 0C’de 3 saat ekilibrasyona tabi tutulduktan sonra sıvı azot buharında donduruldu. Subjektif motilite yönünden 50 ve 100 μg/ml içeren grupların, kontrol grubuna göre belirgin bir üstünlük sağladığı (P<0.05) gözlendi. Ayrıca orta kısım anomalileri ile toplam anormal spermatozoon oranı bakımından kontrol grubuna göre 100 μg/ml içeren gruptaki ve kuyruk kısmındaki anomaliler açısından 50 ve 100 μg/ml içeren gruplardaki azalma önemli (P<0.05) bulunmuştur. H+/E- oranı açısından kontrol grubuna göre tüm antioksidan gruplarındaki artış, tail lenght, tail DNA ve tail moment bakımından kontrol grubuna göre 100 μg/ml CAPE içeren gruptaki azalmalar ve kontrol grubuna göre spermatozoon TAS düzeylerinde 10 ve 100 μg/ml CAPE içeren gruplardaki artış istatistikî olarak önemli (P<0.05) bulundu. Sonuç olarak yaptığımız çalışmada koç spermanın saklanmasında spermaya katılan farklı dozlardaki CAPE'in 100 μg/ml dozu spermatozoon motilite, anormal spermatozoon oranı, HOST-Eozin test oranı, oksidatif stres ve DNA hasarı yönünden kontrol grubu ve diğer gruplara göre en iyi korumayı sağladığı görüldü.

Keywords

CAPE (caffeic acid phenethyl ester), dondurma, DNA hasarı, koç, oksidatif stres

Supporting Institution

Afyon Kocatepe Üniversitesi Bilimsel Araştırmalar Proje Koordinasyon Birimi

Project Number

18.KARİYER.133

The effects of CAPE (caffeic acid phenethyl ester) addition on spermatological parameters, oxidative stress and DNA damage after frozen-thawed process in ram semen.

Abstract

The aim of this study is to investigate the effects of different doses caffeic acid phenethyl ester (CAPE) adding to extender on some spermatological parameters, oxidative stress and DNA damage during post-thawed of ram semen. Ejaculates were collected from five Merino rams using an artificial vagina ones a week and this process was repeated six times. Ejaculates were split into four aliquots and diluted to a final concentration of 150x106 spermatozoa/ml with the base extender containing antioxidant (10 µg/ml, 50 µg/ml and 100 µg/ml)and no additive (control). All samples were cooled to 4°C and equilibrated for 3 h then were loaded into 0.25 ml straws and frozen using a liqued nitrogen vapour and plunged into liquid nitrogen. In terms of subjective motility, the groups containing 50 and 100 μg / ml were found to be significantly superior (P <0.05) compared to the control group. In addition, in terms of mid-piece anomalies and total abnormal spermatozoon rate, the decrease in the group containing 100 μg / ml compared to the control group and in the groups containing 50 and 100 μg / ml in terms of tail anomalies was found to be significant (P <0.05). The increase in all antioxidant groups in terms of H + / E- ratio compared to the control group, decreases in the group containing 100 μg / ml CAPE compared to the control group in terms of tail length, tail DNA and tail moment, and the spermatozoon TAS levels in the groups containing 10 and 100 μg / ml CAPE compared to the control group. the increase was statistically significant (P <0.05). In conclusion, in our study, it was observed that the 100 μg / ml dose of CAPE in different doses added to the sperm in the storage of ram semen provided the best protection compared to the control group and other groups in terms of spermatozoon motility, abnormal spermatozoon rate, HOST-Eosin test rate, oxidative stress and DNA damage.

Keywords

CAPE (caffeic acid phenethyl ester), frozen, DNA damage, ram, oxidative stres

Project Number

18.KARİYER.133

References

• KAYNAKLAR Abdallah, F.B., Fetoui, H, Zribi, N., Fakhfakh, F, Keskes, L. (2012). Protective role of caffeic acid on lambda cyhalothrin-induced changes in sperm characteristics and testicular oxidative damage in rats. Toxicol Ind Health, 28, 639-47.
• Alday-Provencio, S., Diaz, G., Rascon, L., Quintero, J., Alday, E., Robles-Zepeda, R., Garibay-Escobar, A., Astiazaran, H., Hernandez, J., Velazquez, C. (2015). Sonoran propolis and some of its chemical constituents inhibit in vitro growth of Giardia lamblia trophozoites. Planta Med,81 (9), 742–747.
• Armagan, A., Uzar, E., Uz, E., Yilmaz, H.R., Kutluhan, S., Koyuncuoglu, H.R., Soyupek, S., Cam, H., Serel, T.A. (2008). Caffeic acid phenethyl ester modulates methotrexate-induced oxidative stress in testes of rat. Hum Exp Toxicol, 27, 547-52.
• Avdatek, F., Yeni, D., İnanç, M.E., et al. (2018). Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation. Andrologia,. 50:e12975.
• Ayla, Ş., Tunalı, G., Bilgiç, B.E., Sofuoğlu, K., Özdemir, A.A., Tanrıverdi, G, Özdemir, S., Soner, B.C., Öztürk, B, Karahüseyinoğlu, S, Aslan, E.G., Seçkin I. (2018). Antioxidant activity of CAPE (caffeic acid phenethyl ester) in vitro can protect human sperm deoxyribonucleic acid from oxidative damage. Acta Histochemica, 120(2), 117-121.
• Bankova, V., Dyulgerov, A., Popov, S., Marekov, N., (1987). A GC/MS study of the propolis phenolic constituents. Z Naturforsch, 42, 147-151.
• Celli, N., Dragani, LK., Murzilli, S., Pagliani, T., Poggi, A, (2007). In vitro and in vivo stability of caffeic acid phenethyl ester, a bioactive compound of propolis. J Agric Food Chem, 55, 3398– 3407.
• Ceylan, T., Kaymak, E., Cantürk, F., Yakan, B. (2020). Research on the protective effect of caffeic acid phenethyl ester on testicular damage caused by cisplatin. Turk J Med Sci. 50, 2032-2039.
• Chen, H.C., Chen, J.H., Chang, C., Shieh, C.J. (2011). Optimization of ultrasoundaccelerated synthesis of enzymatic caffeic acid phenethyl ester by response surface methodology. Ultrason Sonochem, 18, 455–459.
• Erboga, M., Kanter, M., Aktas, C., Donmez, Y.B., Erboga, Z.F., Aktas, E., Gurel A. (2016). Anti-Apoptotic and Anti-Oxidant Effects of Caffeic AcidPhenethyl Ester on Cadmium-Induced Testicular Toxicity in Rats. Biol Trace Elem Res,171, 176–184.
• Erel, Ö. (2004). A novel automated method to measure total antioxidant response against potent free radical reactions, Clin Biochem, 37(2), 112-119.
• Erel, Ö. (2005). A new automated colorimetric method for measuring total oxidant status, Clin Biochem, 38(12), 1103-1111.
• Gündoğan, M., Yeni, D., Avdatek, F., Fidan, A.F. (2010). Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim. Reprod. Sci, 122, 200-207.
• Hafez E.S.E. (1987). Preservation and cryopreservation of gamates and embryos. Editor: HAFEZ ESE. Reproduction in Farm animals, 5th edition Lea-Febiger, Philadelphia, page 571-600.
• Huyut, Z., Alp, H.H., Yaman, T., Keleş, Ö.F., Yener, Z., Türkan, F., Ayengin, K. (2020). Comparison of the protective effects of curcumin and caffeic acid phenethyl ester against doxorubicin-induced testicular toxicity. Andrologia, 00:e13919.
• Koltuksuz, U., Irmak, M.K., Karaman, A., Uz, E., Var, A., Ozyurt, H., Akyol, O., (2000). Testicular nitric oxide levels after unilateral testicular torsion/detorsion in rats pretreated with caffeic acid phenethyl ester. Urol. Res, 28 (6), 360–363.
• Mercantep, T., Unal, D., Tümkaya, L., Yazıcı, Z.A. (2018). Protective effects of amifostine, curcumin and caffeic acid phenethyl ester against cisplatin-induced testis tissue damage in rats. Exp Ther Med, 15(4), 3404-3412
• Namula, M., Hirata, M., Wittayarat, F., Tanihara, N., Thi Nguyen, T., Hirano, M., Nii, T. (2018). Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm, Reprod. Domest. Anim. 53, 1600–1604.
• Ogeturk, M., Kus, I., Colakoglu, N., Zararsiz, I., Ilhan, N., Sarsilmaz, M. (2005). Caffeic acid phenethyl ester protects kidneys against carbon tetrachloride toxicity in rats. J. Ethnopharmacol. 97 (2), 273–280.
• Okutan, H., Ozcelik, N., Yilmaz, H.R., Uz, E. (2005). Effects of caffeic acid phenethyl ester on lipid peroxidation and antioxidant enzymes in diabetic rat heart. Clinical Biochemistry, 38, 191– 196.
• Ozyurt, H., Pekmez, H., Parlaktas, B.S., Kus, I., Ozyurt, B., Sarsilmaz, M. (2006). Oxidative stress in testicular tissues of rats exposed to cigarette smoke and protective effects of caffeic acid phenethyl ester. Asian J Androl, 8, 189-93.
• Reuter, S., Gupta, S.C., Chaturvedi, M.M., Aggarwal. B.B., (2010). Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med, 49, 1603-1616.
• Russo, A., Acquaviva, R., Campisi, A., Sorrenti, V., Di Giacomo, C., Virgata, G., Barcellona, M.L., Vanella, A. (2000). Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors. Cell Biol Toxicol, 16, 91-98.
• Russo, A., Longo, R., Vanella, A., (2002). Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin. Fitoterapia, 73(1), S21-29.
• Schafer, S., Holzmann, A. (2000). The use of transmigration and Spermac TM stain to evaluate epididymal cat spermatozoa. Anim. Reprod. Sci. 59, 201-211.
• Soleimanzadeh, A., Talavi, N., Yourdshahi, V.S., Bucak, M.N. (2020). Caffeic acid improves microscopic sperm parameters and antioxidant status of buffalo (Bubalus bubalis) bull semen following freeze-thawing process, Cryobiology. 95, 29-35.
• Song, J.J., Lim, H.W., Kim, K., Kim, K.M., Cho, S., Chae, S.W. (2012). Effect of caffeic acid phenethyl ester (CAPE) on H(2)O(2) induced oxidative and inflammatory responses in human middle ear epithelial cells. Int. J. Pediatr. Otorhinolaryngol. 76 (5), 675–679.
• Tolba, M.F., Azab, S.S., Khalifa, A.E., Abdel-Rahman, S.Z., Abdel-Naim, A.B. (2013). Caffeic acid phenethyl ester, a promising component of propolis with a plethora of biological activities: a review on its anti-inflammatory, neuroprotective, hepatoprotective, and cardioprotective effects. IUBMB Life, 65 (8), 699–709.
• Wang X., Stavchansky S., Kerwin S. M., Bowman P. D., (2010). Structure-activity relationships in the cytoprotective effect of caffeic acid phenethyl ester (CAPE) and fluorinated derivatives: effects on heme oxygenase-1 induction and antioxidant activities. Eur J Pharmacol, 635, 16-22.
• Widjaja A, Yeh TH, Ju YH, (2008). Enzymatic synthesis of caffeic acid phenethyl ester. J Chin Inst Chem Eng, 39, 413-418.
• Yılmaz, H.R, Uz, E., Yeşildağ, A., Özden, A., Sırmalı, R., Agackiran, Y., Vural, H. (2008). Effects of caffeic acid phenethyl ester and erdosteine on radiocontrast media-induced oxidative stress and histopathological changes in rat testicular tissue. Cell Membranes and Free Radıcal Research, 1(2),78-83.