Endoparacite investigation in some fish species with high economic value obtained from Çanakkale

Year 2021, Volume: 6 Issue: 3, 202 - 210, 31.12.2021

Ruhay Aldık , Fikret Çakır , Özlem Yayıntaş , Uğur Altınağaç

https://doi.org/10.31797/vetbio.937180

Abstract

The study is an endoparasitic examination of chub mackerel (Scomber japonicus Houttuyn 1782), bogue (Boops boops Linnaeus 1758), horse mackerel (Trachurus trachurus Linnaeus 1758), sardine (Sardina pilchardus Walbaum 1792), and anchovy (Engraulis encrasicolus Linnaeus 1758) fish taken from Çanakkale fish state as an annual sampling between 01 September 2019- 01 September 2020. One thousand three hundred thirty-seven fishes were sampled. The external morphological examination of the fish brought to the laboratory was made, then the conditions of their visceral organs and their visceral structures were examined by dissection method. Nematodes collected alive from many parts of the body were kept in absolute (99.9%) ethanol. Parasites in ethanol were kept in glycerin for a few days before being examined under the microscope.
Nematodes were first examined morphologically on a stereomicroscope and then under a light microscope for easy species identification. Classical detection methods have been used for species determination. As a result, among the individuals belonging to the Anisakidae family, Anisakis spp. L3 larval stage type 1 and type 2, Anisakis physeteris larval stage, Contracaecum larval stage, Hysterothylacium aduncum L3 larval stage were found. The density, abundance, and prevalence rates of the parasites in the infected fish are determined as a percentage.

Keywords

Anisakidae, chub mackerel, bogue, horse mackerel, Çanakkale, Nematode

Supporting Institution

Canakkale Onsekiz Mart University

Project Number

FBA-2019-2904

Thanks

We thank the Canakkale Onsekiz Mart University Scientific Research Commission (FBA-2019-2904) for financial support.

References

• Akmırza, A. (2001). İstavrit balığında (Trachurus trachurus Linnaeus, 1778) rastlanan parazitlerdeki mevsimsel değişimler, Ege Üniversitesi Su Ürünleri Dergisi, 18, (1-2): 33-37.
• Başaran, B. (2016). ISO 22000 gıda güvenliği yönetim sistemi, Journal of Food and Health Science, 2(1), 9-26.
• Casti, D., Scarano, C., Piras, M.C., Merella, P., Muglia, S., Piras, F., Garippa, G., Spanu, C., Luigi de Santis, E.P. (2017). Occurrence of nematodes of the genus Anisakis in Mediterranean and Atlantic fish marketed in Sardinia. Italian Journal of Food Safety 2017; volume 6:6185.
• Cavallero, S., Magnabosco, C., Civettini, M., Boffo, L., Mingarelli, G., Buratti, P., Giovanardi, O., Fortuna, C.M., Arcangeli, G. (2015). Survey of Anisakis sp. and Hysterothylacium sp. in sardines and anchovies from the North Adriatic Sea. International Journal of Food Microbiology 200 (2015) 18–21. http://dx.doi.org/10.1016/j.ijfoodmicro.2015.01.017.
• Chen, Y-J., Wu, C-C., Yang, J-S. (2015). Occurrence of Anisakis larvae in commercial fish along the northern coast of Taiwan. Research Journal of Parasitology 10 (3): 79-91, 2015. ISSN 1816-4943 / DOI: 10.3923/jp.2015.79.91.
• Elibüyük Şahin, G. (2006). Ankara’da satılan hamsi, istavrit ve mezgit balıklarında Anisakidae enfeksiyonları. Doktora Tezi, Türkiye Cumhuriyeti Ankara Üniversitesi Sağlık Bilimleri Enstitüsü. Parazitoloji Anabilim Dalı.
• Erkan, N., Alakavuk, Ü.D., Tosun, Ş.Y. (2008). Gıda Sanayinde Kullanılan Kalite Güvence Sistemleri, Journal of FisheriesSciences.com, http://www.fisheriessciences.com/fisheries-aqua/quality-assurance-systems-in-foodindustry.pdf.
• FAO (Food and Agriculture Organization Of The United Nations) (2011). Private standards and certification in fisheries and aquaculture, http://www.fao.org/3/i1948e/i1948e.pdf
• Karl, H., Levsen, A. (2011). Occurrence and distribution of anisakid nematodes in Grey gurnard (Eutrigla gurnardus L.) from the North Sea. Food Control 22 (2011) 1634-1638. doi:10.1016/j.foodcont.2011.03.021.
• Keser, R., Bray, R., Oğuz, M., Çelen, S., Erdoğan, S., Doğutürk ,S., Aklanoğlu, G., Martı, B. (2007). Helminth parasites of digestive tract of some teleost fish caught in the Dardanelles at Çanakkale, Turkey, Helminthologia, 44(4): 217 – 221.
• Knoff M., Gonçalves da Fonseca, M.C., Felizardo, N.N., Lúcia dos Santos, A., Carmona de São Clemente, S., Kohn, A., Gomes, D.C. (2017). Anisakidae and Raphidascarididae nematodes parasites of tuna (Perciformes: Scombridae) from State of Rio De Janeiro, Brazil. Neotropical Helminthology, 2017, 11(1), jan-jun: 45-52.
• Köksal, P.S. (2019). Karadeniz’in Sinop kıyılarından yakalanan bazı balık türlerindeki nematod parazit faunasının belirlenmesi. Sinop Üniversitesi Fen Bilimleri Enstitüsü Su Ürünleri Yetiştiriciliği ABD, Yüksek Lisans Tezi, 92s.
• Mackenzie, K., Campbell, N., Mattiucci, S., Ramos, P., Pinto, A. L., Abaunza, P. (2008). Parasites as biological tags for stock identification of atlantic horse mackerel Trachurus trachurus L. Fisheries Research, 89, 136-145.
• Mattiucci, S., Farina, V., Campbell, N., Mackenzie, K., Ramos, P., Pinto, A. L., Abaunza, P., Nascetti, G. (2008). Anisakis spp. larvae (Nematoda: Anisakidae) from atlantic horse mackerel: their genetic identification and use as biological tags for host stock characterization. Fisheries Research, 89, 146-151.
• Mehlhorn, H. (2012). Animal parasites diagnosis, treatment, prevention. 7th Edition.pp 356-378. ISBN 978-3-319-46402-2. ISBN 978-3-319-46403-9 (eBook). DOI 10.1007/978-3-319-46403-9.
• Molina, T. R., Aparicio, P. J., Bienes, H., M., Perez, J.,R., Ruso, M. A., Franco, M. E. (2000). Anisakidosis in flesh fish sold in the north of Cordoba. Rev. Esp. Salud. Publica., 74(5-6): 517-26.
• Morsy K., Badr, A.M., Abdel-Ghaffar, F., El Deeb, S., Ebead, S. (2017). Pathogenic potential of fresh, frozen, and thermally treated Anisakis spp. type II (L3) (Nematoda: Anisakidae) after Oral Inoculation into Wistar Rats: A Histopathological Study. Journal of Nematology 49(4):427–436. 2017.
• Ozer, A., Olguner, A.M. (2013). Karadeniz’in Sinop kıyılarından yakalanan bazı deniz balıklarının metazoan parazitleri. Ege J Fish Aqua Sci 30(3): 93-97 (2013). DOI: 10.12714/egejfas.2013.30.3.01.
• Öztürk, T., Yeşil, A. (2018). Metazoan parasite fauna of the red mullet, Mullus barbatus ponticus Essipov, 1927 in the Sinop coasts of the Black Sea. Turkish Journal of Fisheries and Aquatic Sciences 18: 153-160 (2018). DOI: 10.4194/1303-2712-v18_1_17.
• Pekmezci, G.Z., Onuk, E.E., Bolukbas, C.S., Yardimci, B., Gurler, A.T., Acici, M., Umur, S. (2014). Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Veterinary Parasitology 201 (2014) 82–94. http://dx.doi.org/10.1016/j.vetpar.2014.01.005.
• Pira,s M.C., Tedde, T., Garippa, G., Sanna, D., Farjallah, S., Merella, P. (2014). Molecular and epidemiological data on Anisakis spp. (Nematoda: Anisakidae) in commercial fish caught off northern sardinia (western Mediterranean Sea). Veterinary Parasitology 203 (2014) 237–240. http://dx.doi.org/10.1016/j.vetpar.2014.02.003.
• Pozio, E. (2015). "Foodborne nematodes". Foodborne parasites in the food supply Web. pp. 165–199. doi:10.1016/B978-1-78242-332-4.00008-4. ISBN 9781782423324. Rello, F., Adroher, F., Valero, A. (2008). H.aduncum the only anisakid parisite of sardines (Sardina pilchardus) from the southern and eastern coasts of Spain, Parasitol Res., 104: 117-121.
• Resmi Gazete (2008). Gıda güvenliği ve kalitesinin denetimi ve kontrolüne dair yönetmelik. 26 Eylül 2008 Cuma, Resmî Gazete, Sayı : 27009.
• Roca-Geronès X., Fisa R., Montoliu I., 2018. 6. Biogeography of Anisakis (Anisakidae) and Hysterothylacium (Rhaphidascarididae) nematode species in consumed fish. Recent Advances in Pharmaceutical Sciences VIII, 2018: 95-118 ISBN: 978-81-308-0579-5.
• Setyobudi, E., Jeon, C-H., Lee, C-H., Seong, K-B., Kim, J-H. (2011). Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea. Parasitol Res (2011) 108:585–592. DOI 10.1007/s00436-010-2101-x.
• Sahin A. (2014). Çanakkale yöresinden avlanan kolyoz (Scomber japonicus Houttuyn, 1782), istavrit (Trachurus trachurus (Linnaeus, 1758)) ve sardalya (Sardinella aurita Valenciennes, 1847)’da nematodlarin arastirilmasi. Doktora tezi, Fırat Üniversitesi Fen Bilimleri Enstitüsü, s:100.
• Simsek E., Cıloglu A., Yıldırım A., Pekmezci G.Z., 2018. Identification and molecular characterization of Hysterothylacium (Nematoda: Raphidascarididae) larvae in bogue (Boops boops L.) from the Aegean Sea, Turkey. Kafkas Univ Vet Fak Derg. 24 (4): 525-530, 2018. DOI: 10.9775/kvfd.2018.19482.
• Tayar, M. (2010). Gıda güvenliği, Marmara Belediyeler Birliği, Ekosan matbaacılık, İstanbul
• Tayar, M. (2020). Gıda güvenliği ve COVID-19. Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni, Vol:11, Issue:2, Pages:61–71. https://doi.org/10.38137/vetfarmatoksbulten.765700.
• Tepe, Y. (2011). Trabzon, Rize ve Artvin kıyılarından yakalanan bazı ekonomik öneme sahip teleost balıklarının endohelmint faunası. Doktora Tezi. Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Erzurum.
• Tepe, Y., Oğuz, M.C. (2013). Nematode and acanthocephalan parasites of marine fish of the eastern Black Sea coasts of Turkey. Turk J Zool (2013) 37: 753-760. doi:10.3906/zoo-1206-18.
• Zhang, K., Xu Z., Chen, H-X., Guo, N., Li, L. (2018). Anisakid and Raphidascaridid nematodes (Ascaridoidea) infection in the important marine food-fish Lophius litulon (Jordan) (Lophiiformes: Lophiidae). International Journal of Food Microbiology 284 (2018) 105–111. https://doi.org/10.1016/j.ijfoodmicro.2018.08.002.