Koyunlarda kan BHBA seviyesinin fertilite üzerine etkisi

Year 2022, Volume: 7 Issue: 2, 220 - 227, 31.08.2022

Şükrü Güngör , Haydar Özkara , Muhammed İnanç , Mine Herdoğan , Feyzanur Mart , Hasan Ali Çay , Ramazan Yıldız , Hidir Gümüş , Ahmet Cumhur Akın , Ayhan Ata

https://doi.org/10.31797/vetbio.1117222

Abstract

Bu çalışmada koyunlarda sezon dışı dönemde senkronizasyon öncesi kan BHBA seviyesinin gebelik oranlarına üzerine etkisinin incelenmesi amaçlandı. Bu amaçla, Burdur ilinde bulunan en az 1 doğum yapmış 2-4 yaşlı 100 baş Merinos/Merinos melezi koyun kullanıldı. Senkronizasyon amacıyla hayvanlara progesteron emdirilmiş süngerler 60 mg Medroxy progesteron acetat (MPA) intravaginal olarak 14 gün süre ile uygulandı. Uygulama günü hayvanlardan kan alınarak, BHBA (betahidroksibütirik asit) ölçümü yapıldı. Süngerler çıkartılırken, 500-700 IU aralığında kas içi PMSG uygulaması yapıldı. Süngerlerin çıkarılmasından 24 saat sonra arama koçu yardımı ile östruslar gözlendi ve östrusta olduğu belirlenen hayvanlar elde sıfat yöntemi ile çiftleştirildi. Çiftleştirmeleri takiben 35-45. gün aralığında transrektal prob kullanılarak ultrason yardımı ile gebelikler kaydedildi. Senkronizasyona alınan hayvanların tamamının östrusta olduğu belirlendi ve aşımları gerçekleştirildi. Yapılan gebelik muayenesi sonucunda hayvanların 57 baş hayvan gebe (%57) iken 43 baş (%43) hayvan gebe kalmamıştır. Çalışmada, kan BHBA değerleri 0,12 mmol/L -0,66 mmol/L (n:100) aralığında ölçülmüş olup ortalama 0,35±0,083 mmol/L olarak tespit edilmiştir. Gebe kalan hayvanların kan BHBA seviyesi 0,29±0,005 mmol/L olarak tespit edilirken, gebe kalmayan koyunların kan BHBA seviyesi 0,41±0,073 mmol/L olarak tespit edilmiştir. (p<0,001). Ayrıca çalışmada kullanılan koyunlarda kan BHBA düzeyi ile gebelik oranları arasında güçlü bir negatif korelasyon olduğu belirlenmiştir (r = −0,719, p<0,001). Sonuç olarak literatürde anılan dönemde koyunların fertilite parametreleri ile kan BHBA düzeyi ile arasındaki ilişki hakkında bir çalışma olmaması sunulan çalışmanın literatüre önemli bir katkı sağladığı kanısına varılmıştır. Düşük BHBA seviyelerine sahip olan hayvanlardan daha yüksek bir fertilite başarısı elde edilebileceği, yüksek BHBA seviyesine sahip hayvanların enerji dengelerinin düzenlenerek fertilite oranlarının yükseltilebileceği, yapılan çalışmanın küçükbaş hayvanlarda sürü yönetimi açısından 2 yılda 3 kuzulatma hedefinin sağlanmasında dikkat edilmesi gereken bir kriter olabileceği sonucuna varılmıştır.

Keywords

BHBA, Fertilite, Koyun, Senkronizasyon.

Supporting Institution

TÜBİTAK

Project Number

2209/A 2020-2 1919B012003009

The effect of blood BHBA level on fertility in sheep

Abstract

The aim of this study was the effect of pre-synchronization blood BHBA level on pregnancy rates of sheep in the non-breeding season. For this purpose, 100 Merino/Merino crossbred ewe, aged 2-4 years, who have given one birth before, were used in the study. For synchronization purposes, progesterone containing sponges 60 mg Medroxyprogesteron acetate (MPA) analogue, were administered intravaginally for 14 days. BHBA (betahydroxybutyric acid) measurement was made by drawing blood in each animal on the day of administration. 500-700 IU PMSG was injected at the time of sponge withdrawal. Oestrus was observed 24 hours after sponge withdrawal with the help of teaser ram. Animals which was in estrus were hand mated. Pregnancy status was recorded with the help of ultrasound using the transrectal probe in the interval 35-45 days after mating.
All of the synchronized animals were determined to be in estrus and their mating were performed. As a result of the pregnancy examination, 57 (57%) of the animals became pregnant and 43 (43%) did not become pregnant. Blood BHBA values were measured in the range of 0.12 mmol/L -0.66 mmol/L (n:100) in present study and the average of BHBA as 0.35±0.083 mmol/L was determined. While blood BHBA level of pregnant animals was found as 0.29±0.005 mmol/L, blood BHBA level of non-pregnant sheep was found to be 0.41±0.073 mmol/L, which was statistically significant (p<0.001). In addition, it was determined that there was a strong negative correlation between blood BHBA level and pregnancy rates in sheep (r = −0.719, p<0.001).
As a result, it was concluded that there was no study in the literature about the relationship between the fertility parameters of sheep and the blood BHBA level in the mentioned period, and it was concluded that the presented study made a significant contribution to the literature. It has been concluded that a higher fertility success can be achieved from animals with low BHBA levels, that fertility rates can be increased by regulating the energy balance of animals with high BHBA levels, and that the study can be a criterion to be considered in achieving the target of 3 lambing in 2 years in terms of herd management in sheep breeding.

Keywords

BHBA, Fertility, Sheep, Synchronization

Project Number

2209/A 2020-2 1919B012003009

References

• Ağaoğlu, Z., Akgül, Y. (2006). Geviş Getiren Hayvanların İç Hastalıkları II. Baskı, Medipres Matbaacılık, Malatya, (pp. 405-440).
• Akçapınar, H. (2000). ‘‘Koyun yetiştiriciliği’’. Ankara İsmat Matbacılık Ltd Şti.
• Akdağ, C., Akal, E. (2018). Current Approaches In Control Of Reproduction On The Sheep. Journal of Animal Production, 59(2), 65-75.
• Alaçam, E. (1993). Koyunlarda siklik düzen ve üremenin denetlenmesi. Hayvancılık Araştırma Dergisi, 3, 65-69.
• A.O.A.C. (1990): Association of Official Analytical Chemists. 69-88. In: K Helrich 276 (Ed), Official Methods of Analysis. USA
• Carrier J, Stewart S, Godden S, Fetrow J, Rapnicki P. (2004). Evaluation and use of three cowside tests for detection of subclinical ketosis in early postpartum cows. Journal of Dairy Science, 87(11), 3725–35.
• Crampton, E. W., & Maynard, L. A. (1938). The relation of cellulose and lignin content to the nutritive value of animal feeds. The Journal of Nutrition, 15(4), 383-395.
• Dann, HM., Morin, DE., Bollero, GA., Muphy, MR., Drackley, JK. (2005). Prepartum intake, postpartum induction of ketosis and preparturient disorders affect the metabolic status of dairy cows. Journal of Dairy Science, 88, 3249- 3264.
• Dubuc J., Duffield T.F., Leslie K.E., Walton J.S., LeBlanc S.J. (2010). Risk factors for postpartum uterine diseases in dairy cows. Journal of Dairy Science, 93, (12), 5764-5771.
• Duffield, T. (2006). Minimizing subclinical metabolic diseases. WCDS Advances in Dairy Technology, 18, 43-55.
• Duffield T. (2000). Subclinical ketosis in lactating dairy cattle. Veterinary clinics of north america: Food animal practice,16(2), 231–53.
• Drackley et al., (2001) J.K. Drackley, T.R. Overton, G.N. (2001). Douglas Adaptations of glucose and long-chain fatty acid metabolism in liver of dairy cows during the periparturient period. Journal of Dairy Science, 84, 100-112.
• Goering, H. K., Van Soest, P. J., (1970). Forage Fiber Analyses (Apparatus, Reagents, Procedures, and Some Applications), No. 379. US Agricultural Research Service, Washington, DC.
• Gordon, I. (1997). ‘‘Controlled reproduction in sheep and goats’’. Cambridge CABI Publishing, (pp:355-370). International. New York, USA.
• Gürgöze, SY., Zonturlu, AK., Özyurtlu, N. (2009). Investigation of some biochemical parametersand mineral substance during pregnancy and postpartum period in awassi ewes. Kafkas Universitesi Veteriner Fakültesi Dergisi, 15, 957-963.
• Helrich, K. (1990). In: K Helrich 276 (Ed), Association of Official Analytical Chemists (pp. 69-88). Official Methods of Analysis. USA
• Ingvartsen, K. L., Andersen, J. B. (2000). "Integration of metabolism and intake regulation: a review focusing on periparturient animals." Journal of dairy science, 83.7, 1573-1597.
• Jainudeen, MR., Wahid, H., Hafez, E.S.E. (2000). ‘‘Sheep and Goats’’. In: Hafez ESE, Hafez B. (Editors). Reproduction and Farm Animals 7th Edition. A Wolters Kluver Company Philadelphia, 172-181.
• Janovick, N.A. and Drackley, J.K. (2010). Prepartum dietary management of energy intake affects postpartum intake and lactation performance by primiparous and multiparous Holstein cows. Journal of Dairy Science 93, 3086–3102.
• Leblanc, SJ., Lissemore, KD., Kelton, DF., Duffield, TF., Leslie, KE. (2006). Major advences in disease prevention in dairy cattle. Journal of Dairy Science. 89(4), 1267-1279.
• Lucy, MC. (2007). Fertility in high-producing dairy cows: reasons for decline and corrective strategies for sustainable improvement. Society of Reproduction and Fertility supplement, 64, 237-254.
• Moghaddam, G., Hassanpour, A. (2008). Comparison of blood serum glikose, bhba, blood urea nitrogen and calcium consantrations is pregnant and lambed ewes. Journal of Animal and Veterinary Advances, 7, 308-311.
• Navarre, CB., Pugh, DG. (2002). Diseases of Liver in Sheep and Goat Medicine. 1st Edition, WB Saunders, Philadephia, pp: 97-1045.
• N.R.C., (2007). National Research Council (US), National Research Council, Nutrient Requirements of Small Ruminants. National Academy Press, Washington, DC, USA.
• Ospina P.A, Nydam D.V., Stokol T., Overton T.R. (2010). Associations of elevated nonesterified fatty acids and beta-hydroxybutyrate concentrations with early lactation reproductive performance and milk production in transition dairy cattle in the northeastern United States. Journal of Dairy Science 93(4):1596–603.
• Ölmez, M., Akyüz, E., Öğün, M., Şahin, T., Makav, M., Yörük, M. A., Gökce, G., Boğa, Kuru, B. (2020). ‘‘Gürcü Keçisinde Metabolik Profilin Beslenme Dönemlerine Göre Karşılaştırılması’’. Atatürk Üniversitesi Veteriner Bilimler Dergisi, 15(3), 287-293.
• Özyurtlu, N., Bademkıran, S. (2010). Koyunlarda östrus senkronizasyonu ve östrusu uyarma yöntemleri. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 3, 17-22.
• Ramin, AG., Asri, S., Majdani, R. (2005). Correlations among serum glucose, beta hydroxybutyrate and urea concentrations in non-pregnant ewes. American Journal of Veterinary Research, 57, 265-269.
• Sadjadian, R., Seifi, H., Mohri, M. (2012). Variations of energy biochemical metabolites in periparturient dairy saanen goats. Comparative Clinical Pathology, 22, 449-456.
• Sargison, ND., Scott, PR., Penny, CD., Pirie, RS., Kelly, JM., (1994). Plasma enzymes and metabolites as potential prognostic indices of ovine pregnancy toxaemia—a preliminary study. British Veterinary Journal, 150(3), 271-277.
• Şentürk, S. (2013). Pratik Klinik Laboratuvar Kitabı. 1. Baskı, F. Özsan Matbaacılık Sanayi ve Ticaret Limited Şirketi, Bursa, (pp. 43-57).
• Uçar, M., Gündoğan, M., Özdemir, M., Tekerli, M., Eryavuz, A., Saban, E., Özenç, E. (2002). Değişik ırk koyunlarda Progesteron+eCG ile östrusların senkronize edilmesi ve hayvanlarda Kolesterol ile Progesteron seviyelerinin araştırılması. Veteriner Bilimler Dergisi, 18, 79-85.
• Vinoles, C., Forsberg, M., Banchero, G., Rubianes, E. (2001). Effect of long-term and short term Progestagen treatment on follicular development and pregnancy rate in cylcil ewes. Theriogenology, 55, 993-1004.
• Walsh, R.B.J.S., Walton, J.S., Kelton, D.F., Leblanc, S.J., Leslie, K.E., Duffield, T.F. (2007). The effect of subclinical ketosis in early lactation on reproductive performance of postpartum dairy cows. Journal of Dairy Science, 90, 2788- 2796.
• Wildeus, S. (2000). Current concept in synchronization of estrus: Sheep and goats. Journal of Animal Science, 77, 114.
• Yang, M.Y., Fortune, J.E. (2015). Changes in the transcriptome of bovine ovarian cortex during follicle activation in vitro. American Journal of Physiology-Heart and Circulatory Physiology, 47, 600-612.