Effectiveness of May Grunwald Giemsa staining on tissues fixed with sugar cane molasses

Year 2024, Volume: 9 Issue: 2, 157 - 165, 22.08.2024

Hatice Yaren Kuloğlu , Ahmad Yahyazadeh

https://doi.org/10.31797/vetbio.1499149

Abstract

Formaldehyde is a reactive chemical that reacts randomly with various cellular elements. This substance, which is also found in the natural structure of the organism, is used in many areas, from the industry to household materials, from the production of coatings in dentistry to the fixation of cadavers in laboratories. Formaldehyde is commonly used in laboratories for fixation of tissues. In this study, the staining properties of tissues fixed with low-cost sugar cane molasses, which is one of the natural sugars and organically produced in Turkey, shown by May Grunwald Giemsa staining method were compared with those of tissues fixed with buffered formol-saline. As a result, intense maceration was observed in the skin and small intestines. Considering the chromatin distribution, nucleus separation and cytoplasm staining in other tissues (liver, spleen, kidney, testis, large intestine, brain, cerebellum and lung), tissues fixed with sugarcane molasses showed similar properties to tissues fixed with buffered formal-saline.

Keywords

Fixation, formaldehyde, histology, May Grunwald Giemsa, sugar cane molasses

Ethical Statement

This study was carried out at Selçuk University Reserch Animals Application Center. This research was approved by Selçuk University Experimental Medicine Application and Research Center Animal Experiments Ethics Committee (SÜDAM, Ref No: 2017-14, Date: 03/2017).

Supporting Institution

This study was carried out without support from any organization.

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