Uzun Gün Şartlarında Patates (Solanum tuberosum L.)’in Mikroçoğaltımında Jasmonik Asidin Etkisi

Year 2016, Volume: 26 Issue: 1, 79 - 88, 31.01.2016

Ahmet Kumlay

https://doi.org/10.29133/yyutbd.236446

Cited By: 5

Abstract

Çalışmada in vitro şartlarda meristemden geliştirilen Pasinler, Granola ve Caspar çeşitlerinin tek-boğum kesimleri agarla katılaştırılmış MS ortamında farklı jasmonik asit (JA) konsantrasyonlarında mikroçoğaltıma tabi tutulmuşlardır. Araştırma sonuçları 1.0 µM JA konsantrasyonunun incelenen çoğu bitki özellikleri üzerine önemli oranda bir artışa sebep olduğunu ve bu konsantrasyonun etkisinin kontrol ve diğer bütün JA konsantrasyonlarından daha belirgin olduğunu göstermiştir. En yüksek sürgün (8.00), boğum (19.00), yaprak (19.00) ve kök (15.75) sayıları Caspar çeşidinden ve en uzun sürgün uzunluğu (15.13 cm) ise Granola çeşidinden 1.0 µM JA konsantrasyonundan elde edilmiştir. Bu JA konsantrasyonu Pasinler çeşidinde en uzun kökler (14.95 cm) meydana getirmiştir. En yüksek bitki yaş ağırlığı Pasinler (649.38 mg) ve Caspar (630.18 mg) çeşitlerinden, en fazla bitki kuru ağırlığı ise Pasinler (98.58 mg),  Caspar (93.89 mg) ve Granola (79.03 mg) çeşitlerinden ve 1.0 µM JA konsantrasyonundan elde edilmiştir. JA uygulaması mevsime bağlı kalmaksızın bütün yıl boyunca genç bitkiciklerin çoğaltımını teşvik ettiğinden, yeni ticari patates çeşitlerinin ticari olarak hızlı bir şekilde çoğaltımına imkan sağlar. Bu sonuçlar, ayrıca, ekonomik öneme sahip patates çeşitlerinin in vitro şartlarda mikroçoğaltımı ve mikro yumruların kütlesel üretim çalışmalarına da temel teşkil edebilir.   

Keywords

in vitro, Jasmonik asit, Mikroçoğaltım, Patates, Solanum tuberosum, Doku kültürü

The Effect of Jasmonic Acid on the Micropropagation of Potato (Solanum tuberosum L.) under Long Days Conditions

Abstract

Meristem derived in vitro plantlets of potato (Solanum tuberosum L.) cultivars Pasinler, Granola, and Caspar were micropropagated on agar-solidified MS medium containing different concentrations of jasmonic acid (JA)using single-node stem cuttings. The results of the study showed that inclusion of 1.0 µM JA in the MS medium provided a substantial and visible increase in most of the plantlet characteristics studied and the effect of this concentration was more pronounced compared to the control and all other JA concentrations. The highest number of shoots (8.00), nodes (19.00), leaves (19.00), and roots (15.75) on cv. Caspar and a maximum shoot length (15.13 cm) was observed on cv. Granola from 1.0 µM JA concentration. This concentration of JA also helped to induce longest roots (14.95 cm) on cv. Pasinler. The highest plantlet fresh weight from cv. Pasinler (649.38 mg), and cv. Caspar (630.18 mg), and maximum dry weight of plantlets from cv. Pasinler (98.58 mg), cv. Caspar (93.89 mg) and cv. Granola (79.03 mg) were noted on MS medium containing 1.0 µM of JA. Since the application of JA accelerates the multiplication of young plantlets throughout the year without depending on the season, this allows fast commercial propagation of new potato cultivars. These results may also serve as basis for the mass production of these economically important potato cultivars through in vitro micropropagation techniques and microtuberization studies

Keywords

In vitro, Jasmonic acid, Micropropagation, Potato, Solanum tuberosum, Tissue culture

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