Validity Control of Markers Used in Molecular Marker Assisted Selection in Tomato

Year 2022, Volume: 32 Issue: 2, 300 - 309, 30.06.2022

Ceylan Uçar , Suat Şensoy

https://doi.org/10.29133/yyutbd.1039398

Cited By: 2

Abstract

This study aimed to check the validity of the SCAR and CAPS markers developed for certain diseases and pests on some tomato cultivars and genotypes in molecular marker-assisted selection. For this purpose, developed molecular markers for resistance were tested for tomato wilt virus (TSWV), Fusarium wilt (FOL), Tomato leaf curl virus (TYLCV), and root-knot nematode (RKN). SCAR Scr-001 markers for TSWV, TAO1 CAPS marker, and P743DF1-P743DR1, P743DF3- SCAR P6-25 markers for FOL; Scar P6-25 marker which TYLCV; SCAR Mi-23 and PMI, of RKN, CAPS APS and C8B markers for P743DR3, P743DF1-P743DR1, At2F- ToMV were selected. These selected markers were screened in 24 tomato genotypes, 9 of which were commercial and 12 local genotypes as well as the control group, Mountain Merit, NCICELBR, and NCI123S. SCAR Scr-001 marker for TSWV; TAO1 CAPS marker and P743DF3-P743DR3, P743DF1-P743DR1, At2-F-At2-R SCAR markers for FOL; P6-25 SCAR marker for TYLCV; and SCAR Mi-23 and PMI markers for RKN gave results. In this context, it was concluded that the mentioned markers could be efficiently used in marker-assisted selection studies in tomatoes.

Keywords

CAPS, SCAR, Solanum lycopersicum L, Molecular marker assisted selection

Project Number

2015-FBE-YL277

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