Validity Determination of Some Molecular Markers Used in Melon Breeding

Year 2022, Volume: 32 Issue: 2, 424 - 435, 30.06.2022

Mürşide Hatipoğlu , Suat Şensoy

https://doi.org/10.29133/yyutbd.1068346

Cited By: 1

Abstract

The present work aimed to validate some molecular markers (AM, FM, Fom2-R408, Fom2-S342, SCAPB051046, SCOPE14541, T1, T1ex, M3A and M3a SCAR markers and Fom1R-Fom1S, CAPS-Dde I, CAPS2, CAPS3 and EX1-C170T CAPS markers) developed for melon breeding in the literature on some melon cultivars and genotypes in Turkey with the aid of marker-assisted selection. For this purpose, these molecular markers developed for resistance to Fusarium oxysporum f. sp. melonis (FOM), Cucumber mosaic virus (CMV) and powdery mildew or sex determination have been tested. AM, FM, Fom2-R408 and Fom2-S342, (SCAR) and Fom1R-Fom1S, CAPS2, and CAPS3 (CAPS) markers for FOM; SCAPB051046 and SCOPE14541, (SCAR) for CMV; CAPS-Dde I for powdery mildew; T1, T1ex, CAPS EX1-C170T, M3a and M3A markers for sex determination were employed. These selected markers were examined in 24 melon genotypes, 11 of which were commercial F1 cultivars. The results were obtained from FM, Fom2-R408 and Fom2-S342 SCAR markers and Fom1R-Fom1S CAPS marker for Fusarium wilt disease and SCAR SCOPE14541 for CMV. In this context, it is seen that the resultant SCAR and CAPS markers could be used effectively in MAS studies.

Keywords

Cucumis melo L., CAPS, SCAR, MAS, Melon

Project Number

2016-FBE-YL011

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