Propargite (Akarisit)’nin Sublethal Dozlarının Cyprinus carpio Linnaeus, 1758’da Biyokimyasal Kan Parametrelerine Etkisi

Yıl 2019, , 325 - 339, 15.09.2019

Arınç Tulgar , Ekrem Şanver Çelik

https://doi.org/10.22392/actaquatr.527373

Öz

Bu araştırmada organosülfürlü bir pestisit olan propargite çalışılmış ve bu pestisitin sazan balıklarının (Cyprinus carpio) biyokimyasal kan parametreleri üzerine olan etkisine bakılmıştır. Bu parametreler serumda glikoz (GLU), toplam protein (TP), albümin (ALB), globulin (GLOB), laktat dehidrogenaz (LDH), alkalen fosfataz (ALP), kreatin kinaz (CK), glutamik pirüvik transaminaz (GPT), glutamik oksaloasetik transaminaz (GOT), kalsiyum (Ca), magnezyum (Mg), fosfor (P), demir (Fe), klor (Cl), trigliserit (TG), düşük yoğunluklu lipoprotein (LDL), yüksek yoğunluklu lipoprotein (HDL) ve kolesterol (CHOL) şeklindedir. Bu değerlerin ölçümü için balıklar kontrol (Sadece dinlendirilmiş çeşme suyu), düşük (0,04125 mg L-1), orta (0,0825 mg L-1) ve yüksek (0,165 mg L-1) doz propargite konsantrasyonlarına 14 gün süre ile maruz bırakılmışlardır. Deneme 3 tekerrürlü gerçekleştirilirken, örnek balıklar denemenin 0,7 ve 14. günlerinde alınmışlardır.

Anahtar Kelimeler

Pestisit, propargite, Cyprinus carpio, biyokimya

Effect of Sublethal Doses of Propargıte (Acarıcıde) to Bıochemıcal Blood Parameters of Cyprinus carpio Linnaeus, 1758

Öz

In this research, an organosulfide pesticide propargite was studied and the effect of this pesticide on the biochemical blood parameters of carp fish (Cyprinus carpio) was investigated. These parameters can be listed as serum glucose (GLU), totel protein (TP), albumin (ALB), globulin (GLOB), lactate dehydrogenase (LDH), alkalane phosphatase (ALP), creatine kinase (CK), glutamate pyruvate transaminase (GPT), glutamate oxaloacetate transaminase (GOT), calcium (Ca), magnesium (Mg), phosphor (P), iron (Fe), chlor (Cl), triglyceride (TG), low density lipoprotein (LDH), high density lipoprotein (HDL) and cholesterol (CHOL). For analysing these parameters, fish were exposed to control (only rested fountain water), low (0.04125 ppm), medium (0.0825 ppm) and high (0.116 ppm) dose propargite concentrations for 14 days. While the experiment was carried out with 3 replications, sample fishes were taken on the days 0, 7 and 14 of the experiment

Anahtar Kelimeler

Pesticide, propargite, Common carp, biochemistry

Kaynakça

• Agrahari, S., Pandey, K.C., & Gopal, K. (2007). Biochemical alteration induced by monocrotophos in the blood plasma of fish, Channa punctatus (Bloch). Pesticide Biochemistry and Physiology, 88, 268-272. doi: 10.1016/j.pestbp.2007.01.001
• Ahmad, Z. (2011). Acute toxicity and hematological change in common Carp (Cyprinus carpio) caused by diazinon exposure. African Journal of Biotechnology, 10(63), 13852-13859. doi: 10.5897/ajb11.1247
• Banaee, M., Sureda, A., Mirvaghefi, A.R., & Ahmadi, K. (2011). Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and Phsiology, 99, 1-6. doi: 10.1016/j.pestbp.2010.09.001
• Borges, A., Scotti, L.V., Siqueira, D.R., Zanini, R., Amaral, F., Jurinitz, D.F., & Wassermann, G.F. (2007). Changes in hematological and serum biochemical values in jundia Rhamdia quelen due to sublethal toxicity of cypermethrin. Chemosphere, 69, 920-926. doi: 10.1016/j.chemosphere.2007.05.068
• Bricknell, I.R., Bowden, T.J., Bruno, D.W., MacLachlan, P., Johnstone, R., & Ellis, A.E. (1999). Susceptibility of atlantic halibut, Hippoglossus hippoglossus (L). to infection with typical and atypical Aeromonas salmonicida. Aquaculture, 175(1-2), 1-13. doi: 10.1016/s0044-8486(99)00025-3
• Chandrasekar, S., & N, Jayabalan. (1993). Hematological responses of the common Carp, Cyprinus carpio L. exposed to the pesticide endosulfan. Asian Fisheries Science, 6, 331-340.
• El-Sayed, Y.S., Saad, T.T., & El-Bahr, S.M. (2007). Acute intoxication of deltamethrin in monosex Nile tilapia, Oreochromis niloticus with special reference to the clinical, biochemical and haematological effects. Environmental Toxicology and Pharmacology, 24, 212-217. doi: j.etap.2007.05.006
• EPA. (2001). Reregistration Eligibility Decision (RED) for Propargite. Alıntılanma Adresi, hhtp://www.epa.gov/oppsrrd1/REDs/propargite_red.pdf. (09.10.2015).Fırat, Ö., Cogun, H.Y., Yüzereroğlu, T.A., Gök, G., Fırat, Ö., Kargin, F., & Kötemen, Y. (2011). A comparative study on the effects of a pesticide (Cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiology Biochemistry, 37, 657-666. doi: 10.1007/s10695-011-9466-3
• Funk, S. (2013). Propargite. Alıntılanma Adresi, http://www.fao.org/fileadmin/templates/agphome/documents(Pests_Pesticides/JMPR/Evaluation02(propargiteevaljj.pdf (23.09.2014).
• Gholami-Seyedkolaei, S.J., Mirgavhefi, A., Farahmand, H., & Kosari, A.A. (2013). Effect of a glyphosate-based herbicide in Cyprinus carpio: assessment of acetylcholinesterase activity, hematological responses and serum biochemical parameters. Ecotoxicology and Environmental Safety, 98, 135-141. doi: 10.1016/j.ecoenv.2013.09.011
• Giron-Perez, M.I., Barcelos-Garcia, R., Vidal-Charez, Z.G., Romero-Banuelos, C.A., & Robledo-Marenco, M.L. (2006). Effect of chlorpyrifos on the hematology and phagocytic activity of Nile tilapia cells (Oreochromis niloticus). Toxicology Mechanisms and Methods, 16, 495-499. doi: 10.1080/15376510600751988
• Jyothi, B., & Narayan, G. (1999). Certain pesticide-induced carbonhydrate metabolic disorders in the serum of freshwater fish Clarias batrachus (Linn). Food and Chemical Toxicology, 37, 417-421. doi: 10.1016/s0278-6915(99)00020-4
• Kumar, N., Prabhu, P.A.J., Pal, A.K., Remya, S., Aklakur, Md., Rana, R.S., Gupta, S., Raman, R.P., & Jadhao, S.B. (2011). Anti-oxidative and immuno-hematological status of tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pesticide Biochemistry and Phsiology, 99, 45-52. doi: 10.1016/j.pestbp.2010.10.003
• Logan, M. (2010). Biostatistical Design and Analysis Using r: A Practical Guide. A practical guide. London: Wiley-Blackwell.
• Medlineplus. (2014). Albumin-blood (serum). Alıntılanma Adresi, http://www.nlm.nih.gov/medlineplus/ency/article/003480.htm (07.08.2014).
• Morrison, G. (1990). Serum Chloride. Alıntılanma Adresi: http://www.ncbi.nlm.nih.gov/books/NBK309/ (17.09.2014).
• Muralidharan, L. (2014). Impact of fenthion on ionic regulation in the blood of freshwater fish, Cyprinus carpio (Linn). Journal of Environmental Science, Toxicology and Food Technology, 8(1), 63-70. doi: 10.9790/2402-08116370
• Naveed, A., Venkaeshwarlu, P., & Janalah, C. (2011). Biochemical alteration induced by triazophos in the blood plasma of fish, Channa punctatus (Bloch). Annals of Biological Research, 2(4), 31-37.
• Nieves-Puigdoller, K., Björnsson, B.T., & McCormick, S.D. (2007). Effects of hexazinone and atrazine on the phsiology and endocrinology of smolt development in Atlantic salmon. Aquatic Research, 2(4), 31-37. doi: 10.1016/j.aquatox.2007.05.011
• Ozawa, E., Hagiwara, Y., & Yoshida, M. (1999). Creatine kinase, cell membrane and duchenne muscular dystrophy. Molecular and Cellular Biochemistry, 190, 143-151. doi: 10.1007/978-1-4615-5543-8_18
• Pal, S.K., & Das Gupta, S.K. (1994). Pest Control, Alıntılanma Adresi, http://www.icrisat.org/what-we-do/learning-opportunities/Isu-pdfs/sds.15.pdf (12.08.2014).PAN. (2013). Propargite-Toxicity, Use, Water Pollution Potential. Ecological Toxicity and Regulatory Information. Alıntılanma Adresi, http://www.pesticideinfo.org/Detail_chemical.jsp?Rec_Id=PC34266#ChemID (14.02.2014).
• PMEP. (2013). Propargite (Omite, Comite) Chemical Fact Sheet 9/86. Alıntılanma Adresi, http://pmep.cce.cornell.edu/profiles/Insect-mite/merinphos-propargite/propargite/insect-prof-propargite.html (10.08.2014).
• Ramesh, M., & Saravanan, M. (2008). Haematological and biochemical responses in a freshwater fish Cyprinus carpio exposed to chlorpyrifos. International Journal of Integrative Biology, 3(1), 80-83.
• Saravanan, M., Kumar, K.P., & Ramesh, M. (2011). Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pesticide Biochemistry and Physiolog, 100, 206-211. doi: 10.1016/j.pestbp.2011.04.002
• Sathessshkumar, P., Ananthan, G., Kumar, D.S., & Jagadeesan, L. (2011). Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar Estuary, India. Comprehensive Clinical Pathology, 21(6), 1187-1191. doi: 10.1007/s00580-011-1259-7
• Satyanarayan, S., Bejankimar, R.S., Chaudhan, P.R., Kotangale, J.P., & Satyanarayan, A. (2004). Impact of some chlorinated pesticides on the haematology of the fish Cyprinus carpio and Puntius ticto. Journal of Environmental Sciences, 16(4), 631-634.
• Shamoushaki, M.N., Soltani, M., Kamali, A., Imanpoor, M.R., Sharifpour, I., & Khara, H. (2012). Effects of organophosphate on some haematological and biochemical changes in Rutilus frisii Kutum (Kamensky, 1901) male brood stocks. Iranian Journal of Fisheries Sciences, 11(1), 105-117.
• Smith, C.J., Shaw, B.J., & Handy, R.D. (2007). Toxicity of single walled carbon nanotubes to rainbow trout (Oncorhynchus mykiss): respiratory toxicity, organ pathologies, and other physiological effects. Aquatic Toxicolog, 82, 94-109. doi: 10.1016/j.aquatox.2007.02.003
• Tulgar, A. (2014). Propargite (Akarisit)’nin Sublethal Dozlarının Sazan Balığı (Cyprinus carpio, Linnaeus, 1758)’nda Kan Parameterelerine Etkisi ve Kas Dokusundaki Birikimi. Doktora Tezi. Çanakkale Onsekiz Mart Üniversitesi, Çanakkale, Türkiye.
• Tulgar, A., & Çelik, E.Ş. (2015). Effects of sublethal concentrations of propargite to White blood cells of common carp (Cyprinus carpio, Linnaeus, 1758). Marine Science and Technology Bulletin, 4(1), 13-17.
• Turner, L. (2002). Propargite Analysis of Risks to Endangered and Threatened Salmon and Steelhead. Alıntılanma Adresi, http://www.epa.gov/espp/litstatus/effects/Propargite-an alysis.pdf (25.10.2014).
• Val, A.L.., De Menezes, G.C., & Wood, C.M. (1998). Red blood cell adrenergic responses in amazonian teleost. Journal of Fish Biology, 52, 83-93. doi: 10.1111/j.1095-8649.1998.tb01554.x
• Vasantharaja, C., Pugazhendy, K., & Meenambal, M. (2012). Protective role of Cardiospermum halicacabum against the cypermethrin toxicity on the selected biochemical indices in serum activity in Cirrhinus mrigala (Hemilton). Journal of Pharmacy Research, 5(5), 2595-2598.
• Velisek, J., Dobsikova, R., Svobodova, Z., Modra, H., & Luskova, V. (2006). Effect of deltamethrin on the biochemical profile of common carp (Cyprinus carpio L.). Bulletin Environmental Contamination Toxicology, 76, 992-998. doi: 10.1007/s00128-006-1016-9
• Velisek, J., Svobodova, Z., & Machova, J. (2009a). Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Fish Physiology and Biochemistry, 35, 583-590. doi: 10.1007/s10695-008-9258-6
• Velisek, J., Svobodova, Z., Piackova, V., & Sudova, E. (2009b). Effects of acute exposure to metribuzin on some hematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Bulletin Environmental Contamination Toxicology, 82, 492-495. doi: 10.1007/s00128-009-9648-1
• Xu, S. (2001). Environmental Fate of Propargite. Alıntılanma Adresi, fhttp://www.cdpr.ca.gov/docs/emon/pubs/fatememo/propargite.pdf (08.07.2013).
• Zhao, Z., Zhang, L., Wu, J., & Fan, C. (2013). Residual levels, tissue distribution and risk assessment of organochlorine pesticides (OCPs) in edible fishes from Taihu Lake, China. Environmental Monitoring and Assessment, 185, 9265-9277. doi: 10.1007/s10661-013-3249-5