Bazı Bakteriyel Balık Patojenlerinde Biyofilm Oluşumunun Farklı İn Vitro Metodlarla Tespiti

Yıl 2019, , 378 - 390, 15.09.2019

Fethi Filik , Ayşegül Kubilay

https://doi.org/10.22392/actaquatr.537796

Cited By: 5

Öz

Bu
çalışmada, fenomen bir virülens faktörü olan biyofilmin bazı bakteriyel balık
patojenlerinden Staphylococcus warneri,
Aeromonas sobria, Yersinia ruckeri, Flavobacterium psychrophilum, Vibrio
anguillarum bakterileri tarafından üretilmesi tüp, mikroplak ve kongo red
agar yöntemleriyle araştırılmıştır.

Tüp yöntemi ile biyofilm oluşum
dereceleri tüpün iç yüzeyinde oluşan film tabakasının kalınlığına göre sırayla F. psychrophilum’da (++), A. sobria’da
(++), S. warneri’de (+), Y. ruckeri’de (-), V. anguillarum’da (-) olarak tespit
edilmiştir. Mikroplaklarla yapılan biyofilm testinde plaklarda oluşan biyofilm
negatif kontrolde 0,048, S. warneri’de
0,121, A. sobria’da 0,158, Y. ruckeri’de 0,071, F. psychrophilum’da 0,172, V.
anguillarum’da 0,212 absorbans değeri tespit edilmiştir. Mikroplak testinde
biyofilm oluşumu sırayla V. anguillarum,
F. psychrophilum, A. sobria, S. warneri, Y. ruckeri olarak
belirlenmiştir. Kongo red agar testinde biyofilm (slime) oluşturan bakterilerin
koloni morfolojisinin renk değiştirmesine göre biyofilm oluşumu sırayla V. anguillarum, A. sobria, S. warneri,
F. psychrophilum, Y. ruckeri olarak belirlenmiştir. Kongo red agarda kuru
kristalize siyah koloni üreterek en güçlü biyofilm oluşumu V. anguillarum, A. sobria, S. warneri’de bulunmuştur. Pembe
renkli koloni üreten F. psychrophilum ve
Y. ruckeri bakterileri ise bu teste
biyofilm oluşumu göstermemiştir.

     Sonuç
olarak; biyofilm kaynaklı enfeksiyonlar balıklar üzerinde ölümcül etki
göstermektedir. Bu etkilerin araştırılması ve durdurulması balık ölümlerinin
azaltılmasında önemlidir. Bakteriyel balık patojenlerinden olan S. warneri, A. sobria, V.
anguillarum, F. psychrophilum, Y.
ruckeri’nin araştırmamızda oldukça güçlü biyofilm oluşturdukları farklı
yöntemlerle tespit edilmiştir. 

Anahtar Kelimeler

Bakteriyel balık patojenleri, biyofilm, tüp yöntemi, mikroplak yöntemi, kongo red agar yöntem

Kaynakça

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