Crocus cancellatus subsp. mazziaricus (Herbert) Mathew Bitki Ekstraktının Avrupa Deniz Levrek Balığı (Dicentrarchus labrax, L.1758) Doğal Bağışıklık Sistemi Üzerine Etkisi

Yıl 2020, , 148 - 157, 01.03.2020

Canan Öntaş , Gülşen Uluköy , Esin Baba , Ramazan Mammadov

https://doi.org/10.22392/actaquatr.622606

Cited By: 1

Öz

Bu çalışmada Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geofit bitki ekstraktı iki farklı konsantrasyonda (0,5 ve 2 mg/balık) intraperitonal enjeksiyon yöntemiyle Avrupa deniz levreğine (Dicentrarchus labrax, L. 1758) uygulanmış ve bağışıklık sistemine etkisi araştırılmıştır. Enjeksiyon sonrası 7., 14., 21., 28. ve 35. günlerde balıklardan kan örnekleri alınmıştır. Doğal bağışıklık ile ilgili parametrelerden olan toplam lökosit sayısı, hematokrit seviye, NBT(+) hücre sayısı, lizozim aktivitesi ve serum total protein miktarındaki değişimler tespit edilmiştir. Crocus cancellatus subsp. mazziaricus uygulanan deneme gruplarında kontrol grubuna kıyasla doğal bağışıklık sisteminin aktifleşerek, NBT(+) hücre sayısının 21. günde, toplam lökosit sayısının 14.günde, serum total protein miktarının 14. ve 21. günlerde ve serum lizozim aktivitesinin 21. günde en yüksek seviyeye ulaştığı tespit edilmiştir. Sonuç olarak, 2 mg/balık dozunda uygulanan C. cancellatus subsp. mazziaricus ekstraktının 0,5 mg/balık dozuna kıyasla daha etkin olduğu bulunmuştur.

Anahtar Kelimeler

İmmunostimulant, bitki ekstraktı, bağışıklık sistemi, Crocus cancellatus subsp. mazziaricus, Dicentrarchus labrax

Destekleyen Kurum

tübitak

Proje Numarası

104V126

Teşekkür

Bu çalışma, TUBİTAK tarafından desteklenen 104V126 no’lu kariyer projesi içerisinde gerçekleştirilmiş olup “Crocus cancellatus (Herbert) Mathew bitkisinin levrek balığının (Dicentrarchus labrax, Linnaeus 1758) spesifik olmayan immün sistem üzerine etkisinin araştırılması” isimli yüksek lisans tezi olarak savunulmuştur.

Effects of Crocus cancellatus subsp. mazziaricus (Herbert) Mathew Plant Extract on the Innate Immune System of European Sea bass (Dicentrarchus labrax, L.1758)

Öz

In this study, Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geophyte plant extract was applied to European sea bass (Dicentrarchus labrax, L. 1758) in two different concentrations (0.5 and 2 mg/fish) by intraperitoneal injection method and its effect on the immune system was investigated. After injection on the 7th, 14th, 21st, 28th and 35th days, blood samples were collected from fish. Some of the innate immune related parameters in blood and serum samples such as total leukocyte count, hematocrit level, NBT (+) cell count, lysozyme activity and serum total protein content were determined. C. cancellatus subsp. mazziaricus plant extract treated groups exhibited significant immunostimulatory effect, increased NBT (+) cell count at 21st day, total leucocyte count in 14th day, serum total protein level at 14th and 21st day and serum lysozyme activity at 21st day compared with the control group. As a result of plant extract application, the 2 mg/fish group was found to be more effective than the 0.5 mg/fish group.

Anahtar Kelimeler

Immunostimulant, plant extract, immune system, Crocus cancellatus subsp. mazziaricus, Dicentrarchus labrax.

Proje Numarası

104V126

Kaynakça

• Abdel-Tawwab, M., Adeshina, I., Jenyo-Oni, A., Ajani, E. K., & Emikpe, B. O. (2018). Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection. Fish and Shellfish Immunology.
• Abdel-Tawwab, M., Samir, F., Abd El-Naby, A. S., & Monier, M. N. (2018). Antioxidative and immunostimulatory effect of dietary cinnamon nanoparticles on the performance of Nile tilapia, Oreochromis niloticus (L.) and its susceptibility to hypoxia stress and Aeromonas hydrophila infection. Fish and Shellfish Immunology.
• Adel, M., Safari, R., Pourgholam, R., Zorriehzahra, J., & Esteban, M. Á. (2015). Dietary peppermint (Mentha piperita) extracts promote growth performance and increase the main humoral immune parameters (both at mucosal and systemic level) of Caspian brown trout (Salmo trutta caspius Kessler, 1877). Fish and Shellfish Immunology.
• Awad, E., & Awaad, A. (2017). Role of medicinal plants on growth performance and immune status in fish. Fish and Shellfish Immunology, 67, 40–54. Baba, E., Uluköy, G., & Mammadov, R. (2014). Effects of Muscari comosum Extract on Nonspecific Immune Parameters in Gilthead Seabream, Sparus aurata (L. 1758). Journal of the World Aquaculture Society, 45(2), 173–182.
• Baba, E., Uluköy, G., & Mammadov, R. (2014). Effects of Muscari comosum Extract on Nonspecific Immune Parameters in Gilthead Seabream, Sparus aurata (L. 1758). Journal of the World Aquaculture Society, 45(2), 173–182.
• Baba, E. (2014). Gökkuşağı Alabalığı (Oncorhynchus mykiss, Walbaum 1792)’nda Bazı Mantar Türlerinin Spesifik Olmayan İmmün Sistem Üzerine Etkilerinin Araştırılması. Muğla Sıtkı Koçman Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, Muğla.
• Beltrán, J. M. G., Espinosa, C., Guardiola, F. A., & Esteban, M. Á. (2018). In vitro effects of Origanum vulgare leaf extracts on gilthead seabream (Sparus aurata L.) leucocytes, cytotoxic, bactericidal and antioxidant activities. Fish and Shellfish Immunology, 79(April), 1–10.
• Bilen, S., Özkan, O., Alagöz, K., & Özdemir, K. Y. (2018). Effect Of dill (Anethum graveolens) and garden cress (Lepidium sativum) dietary supplementation on growth performance, digestive enzyme activities and immune responses of juvenile common carp (Cyprinus carpio). Aquaculture, 495, 611-616.
• Bradford, M.M. (1976). A rapid method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248.
• Candan, A. & Karataş, S. (2010). Balık Sağlığı. Kalmak Ofset, İstanbul.
• Demers, N. & Christopher, B. (1997). The immediate effects of stres on hormones and plasma lysozyme in rainbow trout. Developmental & Comparative Immunology, 21, 363- 373.
• Dinesh Kumar, G., Karthik, M., Rajakumar, R., & Baskar, K. (2018). Effects of Avicennia marina extracts on Labeo rohita (Ham) challenged with Pseudomonas fluorescens. Biotechnology Research and Innovation, 3, 54-59.
• Done, H. Y., & Halden, R. U. (2015). Reconnaissance of 47 antibiotics and associated microbial risks in seafood sold in the United States. Journal of Hazardous Materials, 282, 10–17.
• Fazio, F. (2019). Fish hematology analysis as an important tool of aquaculture: A review. Aquaculture, 500(October 2018), 237–242.
• Hai, N. Van. (2015). The use of medicinal plants as immunostimulants in aquaculture: A review. Aquaculture, 446, 88–96.
• Iwama, G. & Nakanishi, T. (1996) The Fish İmmun System, Organism, Pathogen and Environment, Academic Press.
• Kav., K & Erganiş., O. (2008). Balıklarda Bağışıklık Sistemi. Veteriner Bilimleri Dergisi, 1, 97-106.
• Laith, A. A., Mazlan, A. G., Effendy, A. W., Ambak, M. A., Nurhafizah, W. W. I., Alia, A. S., Najiah, M. (2017). Effect of Excoecaria agallocha on non-specific immune responses and disease resistance of Oreochromis niloticus against Streptococcus agalactiae. Research in Veterinary Science, 112(April), 192–200.
• Lundén, T., Miettinen, S., Lönnström, L.-G., Lılıus, E.M., & Bylund, G. (1998). Influence of oxytetracycline and oxolinic acid on the immune response of rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 8(3), 217–230.
• Munyemana, F. (2013). Qualitative Phytochemical Screening and Antimicrobial Activity Evaluation of the Bulb Extracts of Gladiolus psittacinus Hook (Iridaceae). Revista Internacional de. 2(1), 14–31.
• Ngugi, C. C., Oyoo-Okoth, E., Mugo-Bundi, J., Orina, P. S., Chemoiwa, E. J., & Aloo, P. A. (2015). Effects of dietary administration of stinging nettle (Urtica dioica) on the growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) challenged with Aeromonas hydrophila. Fish and Shellfish Immunology, 44(2), 533–541.
• Reis Dias, M. K., Oba Yoshioka, E. T., Ruiz Rodriguez, A. F., Ribeiro, R. A., Escócio Drummond Viana Faria, F. S., Almeida Ozório, R. O., & Tavares-Dias, M. (2019). Growth, physiological and immune responses of Arapaima gigas (Arapaimidae) to Aeromonas hydrophila challenge and handling stress following feeding with immunostimulant supplemented diets. Fish and Shellfish Immunology, 84(October 2018), 843–847.
• Ren, X., Pan, L., & Wang, L. (2014). Effect of florfenicol on selected parameters of immune and antioxidant systems and damage indexes of juvenile Litopenaeus vannamei following oral administration. Aquaculture, 432, 106–113.
• Rico, A., Minh, T., Satapornvanit, K., Min, J., Shahabuddin, A. M., Henriksson, P. J. G., & Brink, P. J. Van Den. (2013). Use of veterinary medicines, feed additives and probiotics in four major internationally traded aquaculture species farmed in Asia. Aquaculture, 412–413, 231–243.
• Saeidi asl, M. R., Adel, M., Caipang, C. M. A., & Dawood, M. A. O. (2017). Immunological responses and disease resistance of rainbow trout (Oncorhynchus mykiss) juveniles following dietary administration of stinging nettle (Urtica dioica). Fish and Shellfish Immunology, 71(October), 230–238.
• Sarvi Moghanlou, K., Nasr Isfahani, E., Dorafshan, S., Tukmechi, A., & Aramli, M. S. (2018). Effects of dietary supplementation with Stachys lavandulifolia Vahl extract on growth performance, hemato-biochemical and innate immunity parameters of rainbow trout (Oncorhynchus mykiss). Animal Feed Science and Technology, 237(May 2016), 98–105.
• Sukumaran, V., Chang, S., & Sankar, S. (2016). Fish & Shell fi sh Immunology Role of dietary ginger Zingiber of fi cinale in improving growth performances and immune functions of Labeo rohita fingerlings. Fish and Shellfish Immunology, 57, 362–370.
• Terech-Majewska, E. (2016). Improving disease prevention and treatment in controlled fish culture. Archives of Polish Fisheries, 24(3), 115–165.
• Treves-Brown, K.M. (2000). Applied Fish Pharmacology. Kluwer Academic Puplishers, Dordrecht, The Netherlands.
• TUİK (Türkiye İstatistik Kurumu). (2017). Su Ürünleri İstatistikleri. Retrieved from www.tuik.gov.tr.
• Uribe, C., Folch, H., Enriquez, R., & Moran, G. (2011). Innate and adaptive immunity in teleost fish: a review. Veterinární Medicína, 56(10), 486–503.
• Wang, E., Chen, X., Wang, K., Wang, J., Chen, D., Geng, Y., Wei, X. (2016). Plant polysaccharides used as immunostimulants enhance innate immune response and disease resistance against Aeromonas hydrophila infection in fish. Fish and Shellfish Immunology, 59, 196–202.