Silurus triostegus’un Gonat Dokusu Yağ Asidi İçeriğinin Mevsimsel Değişimi

Yıl 2022, , 24 - 37, 01.03.2022

Semra Kaçar , Mehmet Başhan

https://doi.org/10.22392/actaquatr.934517

Cited By: 1

Öz

Bu çalışmada, Atatürk Baraj Gölü'nden toplanan (Mayıs-Mart ayları arasında) Silurus triostegus'un (Heckel, 1843) gonat dokusu yağ içeriği ve yağ asidi (FA) kompozisyonunun belirlenmesi amaçlamıştır. Gonat dokusu toplam lipid miktarı, dişi ve erkek balıklarda sırasıyla Mayıs ve Eylül aylarında maksimuma ulaşmıştır. Tüm mevsimlerde en fazla bulunan yağ asitleri; doymuş yağ asitleri (SFA) içinde palmitik asit, tekli doymamış yağ asitleri içinde oleik asit, çoklu doymamış yağ asitleri içinde (PUFA); 22:6n-3 (dokosaheksaenoik asit, DHA), 18:3n-3 (linolenik asit), 18:2 n-6 (linoleik asit) ve 20:5n-3 (eikosapentaenoik asit, EPA)’dır. Erkek ve dişi balık türlerinin gonat dokularındaki TAG ve PL yağ asidi bileşimleri farklı bulunmuştur. Dişi ve erkek örneklerde PL'ye kıyasla tüm mevsimlerin TAG'lerinde PUFA'ların oranı daha düşük bulunmuştur. Genel olarak n-3 yağ asitleri yüzdeleri, n-6 yağ asitleri yüzdelerinden daha yüksek belirlenmiştir. Cinsiyete bağlı olarak, incelenen gonad dokusunda bireysel FA'ler arasında nicel farklılıklar vardır.

Anahtar Kelimeler

Silurus triostegus, Atatürk Baraj Gölü, Mevsimsel yağ asidi dağılımı

Destekleyen Kurum

Bu çalışma DÜBAP tarafından desteklenmiştir (

Proje Numarası

Proje No: 08-FF07).

Seasonal Variations on the Fatty Acid Composition in Gonad Tissue of Silurus triostegus

Öz

In this study, it was aimed to determine the fat content and fatty acid (FA) composition in gonad tissue of Silurus triostegus (Heckel, 1843) caught (between May and March) from Atatürk Dam Lake. The amount of total lipid in gonad tissue reached its maximum in female and male fish in May and September, respectively. The most abundant FAs in all-season were palmitic acid amongst saturated fatty acids (SFAs), oleic acid amongst monounsaturated fatty acids, 22:6n-3 (docosahexaenoic acid, DHA), 18:3n-3 (linolenic acid), 18:2 n-6 (linoleic acid), and 20:5n-3 (eicosapentaenoic acid, EPA), amongst polyunsaturated fatty acids (PUFAs). Triacylglycerol and PL fatty acid compositions in gonad tissues of the male and female fish species were found different. A lower proportion of PUFAs was found in the TAG of all seasons compared to PL in female and male samples. Generally, percentages of n-3 fatty acids were determined higher than percentages of n-6 fatty acids. There were quantitative differences between individual FAs in the gonad tissue investigated, depending on the sex.

Anahtar Kelimeler

Silurus triostegus, Atatürk Dam Lake, Seasonal fatty acid composition

Proje Numarası

Proje No: 08-FF07).

Kaynakça

• Akpınar, M.A. (1985). Cyprinus carpio L. (Osteichthyes, Cyprinidae)’nin ergin ve ergin olmayan bireylerinde gonadların total lipit ve yağ asiti bileşimleri. Doktora Tezi. C. Ü. Fen Ede. Fak. Sivas.
• Akpınar, M.A. (1987). Ergin olmayan ve ergin sazanların (Cyprinus carpio L.) gonatlarında total lipid değişimi. C.Ü. Fen- Ede. Fak. Fen Bilimleri Dergisi, 5, 173-190.
• Aras, N.M., Haliloğlu, H.I., Bayır, A., Atamanalp, M., & Sirkecioğlu, A.N. (2003a). Karasu Havzası Yeşildere Çayı Olgun Dere Alabalıkları (Salmo trutta macrostigma, Dumeril, 1858)'nda farklı dokuların yağ asidi kompozisyonlarının karşılaştırılması. Turkish Journal of Veterinary and Animal Science, 27, 887-892.
• Aras, N.M., Haliloğlu, H.I., Ayık, Ö., & Yetim, H. (2003b). Comparison of fatty acid profiles of different tissues of mature trout (Salmo trutta labrax, Pallas, 1811) caught from Kazandere Creek in the Çoruh Region, Erzurum, Turkey. Turkish Journal of Veterinary and Animal Science, 27, 311-316.
• Balçık Mısır, G., Tufan, B., & Köse, S. (2014). Variations in total lipid and fatty acid contents of edible muscle, liver and roes of spotless shad, Alosa immaculata during catching Season in Black Sea. Journal of Aquatic Food Product Technology, 49, 2668-2677. https://doi.org/10.1080/10498850.2013.816402
• Biró, J., Hancz, C., Szabó, A., & Molnár, T. (2009). Effect of sex on the fillet quality of Nile tilapia fed varying lipid sources. Italian Journal of Animal Science, 8 (3), 225-227. https://doi.org/10.4081/ijas.2009.s3.225
• Bozkurt, R. (1994). Atatürk Baraj Gölü ve Baraj Gölü’ne dökülen derelerdeki balıkların sistematiği. Yüksek Lisans Tezi, H. Ü. Fen Bil. Ens.s. 71. Şanlıurfa.
• Cejas, J.R., Almansa, E., Villamandos, J.E., Badia, P., Bolanos, A., & Lorenzo, A. (2003). Lipid and fatty acid composition of ovaries from wild fish and ovaries and eggs from captive fish of white sea bream (Diplodus sargus). Aquaculture, 216 (1-4), 299-313.
• Crowford, R.H., Cusack, R.R., & Parlee, T.R. (1986). Lipid content and energy expenditure in the spawning migration of alewife (Alosa pseudoharengus) and bluelback herring (Alosa aestivalis). Canadian Journal of Zoology, 64, 1902-1907. https://doi.org/10.1139/z86-285
• Falch, E., Størseth, T.R., & Aursand, M. 2006. Multi-component analysis of marine lipids in fish gonads with emphasis on phospholipids using high resolution NMR spectroscopy. Chemistry and Physics of Lipids, 144 (1), 4–16. https://doi.org/10.1016/j.chemphyslip.2006.05.001
• Folch, J., Lees, M., & Sloane-Stanley, G.H.A. (1957). Simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry, 226, 497. . https://doi.org/10.1016/s0021-9258(18)64849-5
• Görgün, S., Akpınar, N., Zengin, G., Akpınar, M.A., Gunlu, A., Güler, G.O., & Aktümsek, A. (2013). Determination of the fatty acid profiles of total, neutral, and polar lipids in some major tissues of Vimba vimba tenella (Nordmann, 1840), from Eğirdir Lake (Isparta, Turkey). Turkish Journal of Zoology, 37, 627–634.509. https://doi.org/10.3906/zoo-1212-33
• Henderson, R.J., & Tocher, D.R. (1987). The lipid composition and biochemistry of freswater fish. Progress in Lipid Research, 26, 281-347. https://doi.org/10.1016/0163-7827(87)90002-6
• Huynh, M.D. (2007). Comparison of fatty acid profiles of spawning and non-spawning Pacific herring, Clupea harengus pallasi. Comparative Biochemistry and Physiology, 146 B, 504-511. https://doi.org/10.1016/j.cbpb.2006.11.023
• Jeong, B.Y., Jeong, W.G., Moon, S.K., & Ohshima, T. (2002). Preferential accumulation of fatty acids in the testis and ovary of cultured and wild sweet smelt Plecoglossus altivelis. Comparative Biochemistry and Physiology, 131 B, 251-259. https://doi.org/ 10.1016/s1096-4959(01)00501-2
• Johnson, R.B. (2009). Lipid deposition in oocytes of teleost fish during secondary oocyte growth. Reviews in Fisheries Science, 17(1), 78-89. https://doi.org/10.1080/10641260802590004
• Kaçar, S., & Başhan, M. (2015). Seasonal Variations on the Fatty Acid Composition of Phospholipid and Triacylglycerol in gonad and liver of Mastacembelus simack. Journal of the American Oil Chemists' Society. 92 (9), 1313-1320. https://doi.org/10.1007/s11746-015-2692-6
• Kaçar, S., & Başhan, M. (2017). Variations in the fatty acid compositions of the liver and gonad tissue of spiny eel (Mastacembelus mastacembelus) from Atatürk Dam Lake. Turkish Journal of Biochemistry, 42(6), 617-623. https://doi.org/10.1515/tjb-2017-0045
• Kara, C., & Çelik, M. (2000). Fatty acid composition of gonad tissue in female and male Chondrostoma regium (Heckel, 1843) living in Ceyhan River, Kahramanmaraş-Turkey. Fen ve Mühendislik Dergisi, 3, 160-166.
• Kayhan, H., Başhan, M., & Kaçar, S. (2015). Seasonal variations in the fatty acid composition of phospholipids and triacylglycerols of brown trout. European Journal of Lipid Science and Technology, 116 (5), 738-744. https://doi.org/10.1002/ejlt.201400152
• Kellner, R.G., & Van Der Kraak, G. (1992). Multifactorial regulation of prostaglandin synthesis in preovulatory goldfish ovarian follicles. Biology of Reproduction, 46, 630-635. https://doi.org/10.1095/biolreprod46.4.630
• Kminkova, M., Winterova, R., & Kucera, J. (2001). Fatty acids in lipids of carp (Cyprinus carpio) tissues. Czech Journal of Food Science, 19, 177-181.
• Kozlova, T.A. (1998). Lipid class composition of benthic-pelagic fishes (Cottocomephorus, Cottoidei) from Lake Baikal. Fish Physiology and Biochemistry, 19, 211-216. https://doi.org/10.1023/A:1007706831689
• Kozlova, T.A., & Khotimchenko, S.V. (2000). Lipids and fatty acids of two pelagic cottoid fishes (Comephorus spp.) endemic to Lake Baikal. Comparative Biochemistry and Physiology, 126 B, 477-485. https://doi.org/10.1016/s0305-0491(00)00207-8
• Medford, B.A., & Mackay, W.C. 1978. Protein and lipid content of gonads, liver and muscle of Northern pike (Esox lucius) in relation to gonad growth. Journal of the Fisheries Research Board of Canada, 35, 213-219. https://doi.org/10.1139/f78-035
• Metin, K., & Akpınar, M.A. (2000). The seasonal variation in total lipid and fatty acid contents of the gonads of Cyprinion macrostomus (Heckel, 1843). Turkish Journal of Biology, 24, 627-634.
• Mustafa, T., & Srivastava, K.C. (1989). Prostaglandins (eicosanoids) and their role in ectothermic organisms. Advances in Comparative Environmental Physiology, 5, 157-207.
• Oymak, S.A., Solak, K., & Ünlü, E. (2001). Some biological characteristics of Silurus triostegus Heckel, 1843 from Atatürk Dam Lake (Turkey). Turkish Journal of Zoology, 25, 139-148.
• Qari, S.A., Moharram, S.G., & Alowaidi, S.A. (2014). Fatty acids Compositions in Male’s Gonads of the Red Sea Fish Rhabdosargus sarba During the Spawning Season. American Journal of Life Sciences, 2 (2), 103-107. https://doi.org/ 10.11648/j.ajls.20140202.21
• Rodríguez-Barreto, D., Jerez, S., Cejas, J.R., Martin, M.V., Acosta, N.G., Bolanos, A., & Lorenzo, A. (2012). Comparative study of lipid and fatty acid composition in different tissues of wild and cultured female broodstock of greater amberjack (Seriola dumerili). Aquaculture, 360, 1-9. https://doi.org/10.1016/j.aquaculture.2012.07.013
• Sargent, J.R. (1995). Origin and functions of eggs lipids: nutritional implications. In: N.R. Bromage and R.J. Roberts (Eds.), Broodstock Management and Egg and Larval Quality. Blackwell Science, London: 353–372.
• Sargent, J.R., & Henderson, R.J. (1995). Marine n-3.polyunsaturated fatty acids. In: Hamilton, R.J.-Eds. Developments in Oils and Fats. Blackie Academic and Professional, London, p. 32-65.
• Sargent, J.R., Tocher, D.R., & Bell, J.G., (2002). In: J.E., Halver; R.W., Hardy (Eds.). "The lipids" Fish Nutrition.3rd edition. Academic Press, San Diego. 181–257.
• Shirai, N., & Wada, S. (2001). Seasonal variation of fatty acid composition of phosphatidylinositol in the dorsal meat, liver and ovary of cultured Japanese catfish Silurus asotus. Fisheries Science, 67, 386-388. https://doi.org/10.1046/j.1444-2906.2001.00239.x
• Shirai, N., Suzuki, H., Toukairin, S., & Wada, S. (2001). Spawning and season affect lipid content and fatty acid composition of ovary and liver in Japanese catfish (Silurus astus). Comparative Biochemistry and Physiology, 129 B,185-195. https://doi.org/10.1016/s1096-4959(01)00378-5.
• Soivio, A., Nıemıstö, M., & Backsröm, M. (1989). Fatty acid composition of Coregonus muksun Pallas: Changes during incubation, hatching, feeding and starvation. Aquaculture, 79,163-168. https://doi.org/10.1016/0044-8486(89)90457-2
• Sushchik, N.N. Gladyshev, M.I., & Kalachova, G.S. (2007). Seasonal dynamics of fatty acid content of a common food fish from the Yenisei River, Siberian grayling, Thymallus arcticus. Food Chemistry, 104 (4), 1353-1358. https://doi.org/ 10.1016/j.foodchem.2007.01.050
• Suzuki, H., Okazaki, K., Hayakawa, S., Wada, S., & Tamura, S. (1986). Influnece of commercial dietary fatty acids on PUFA of cultured freshwater fish and comparison with those of wild fish of the same species. Journal of Agricultural and Food Chemistry, 34, 58-60. https://doi.org/10.1021/jf00067a016
• Tocher, D.R., & Sargent, J.R. (1984). Analyses of lipids and fatty acids in ripe roes of some Northwest Eurpean marine fish. Lipids, 19 (7), 492-499. https://doi.org/ 10.1007/BF02534481
• Tufan, B., Koral, S. & Köse, S. (2011). Changes during fishing season in the fat content and fatty acid profile of edible muscle, liver and gonads of anchovy (Engraulis encrasicolus) caught in the Turkish Black Sea. International Journal of Food Science and Technology, 10, 439-443. https://doi.org/ 10.1111/j.1365-2621.2011.02562.x
• Uysal, K. (2004). Gonad olgunlaşması esnasında sudak (Sander lucioperca) balığının ovaryum ve testislerinin yağ asidi bileşimindeki değişimler. DPÜ Fen Bilimleri Enstitüsü. 7. Sayı.
• Vlaming, V.L.D., Kuris, A., & Parker, F.R. (1978). Seasonal variations of reproduction and lipid reserves in some Subtropical Cyprinodontids, Transaction of the American Fisheries Society, 107 (3), 464-472.
• Wade, M.G., & Van Der Kraak, G. (1993). Regulation of prostaglandins E and F production in the goldfish testes. Journal of Experimental Zoology, 266, 108-115.
• Wiegand, M.D. (1996). Composition, accumulation and utilization of yolk lipids inteleost fish. Reviews in Fish Biology and Fisheries, 6, 259-286.
• Yılmaz. Ö., Konar, V., & Çelik, S. (1995). Elazığ Hazar Gölü’ndeki Capoeta capoeta umbla’nın dişi ve erkek bireylerinde bazı dokularının total lipit ve yağ asidi bileşimleri. Biyokimya Dergisi. 20, 31-42.