Genetic Diversity and Phylogenetic Relatıonshıps of Turkish Local Popcorn (Zea mays everta) Populations By Simple Sequence Repeats (SSRs)

Yıl 2021, Cilt: 27 Sayı: 2, 170 - 178, 04.06.2021

Gülay Zulkadir , Leyla İdikut

https://doi.org/10.15832/ankutbd.626912

Cited By: 1

Öz

Maize (Zea mays everta) is preferred as a good dietary in Turkey and it is important to know its genetic diversity to improve the yield. Genetic markers are very important in determining genetic diversity in popcorn populations. The aim of this study was to evaluate the genetic diversity of landraces popcorn populations by simple sequence repeats (SSR) markers. A hundred seventy five accessions of popcorn from thirty five populations grown in Turkey were analyzed using twenty SSR markers. As a result of molecular analysis, 65 of 66 alleles obtained were showed polymorphisms and the polymorphism rate was 98.5%. The average number of alleles for each SSR loci was 3.3, and this the number of alleles varied from 1 to 5. The average the polymorphism information content (PIC) value was calculated to be 0.57 for SSR locus ranging from 0.00 to 0.89. The number and percentage of polymorphic loci of the genotypes were determined to vary between 29/47% and 43.94/71.21 % and the mean values were calculated as 39.114 and 59.265 % respectively. The value of genetic change in the phylogenetic tree obtained from landraces popcorn populations was determined as 0.05, and the genetic difference among genotypes varied from 14.7 to 97.1%. Among the markers used in the study, it was observed that code ‘phi064’ was the most effective marker for determining genetic diversity in popcorn and the highest allele frequency also on this marker was obtained.

Anahtar Kelimeler

Genetic diversity, landrace, molecular characterization, popcorn, ssr

Destekleyen Kurum

Scientific Research Projects Unit of the Kahramanmaras Sutcu Imam University

Proje Numarası

2014/3-27 D

Teşekkür

This study was supported by the Scientific Research Projects Unit of the Kahramanmaras Sutcu Imam University (Project number: 2014/3-27 D).

Kaynakça

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