Determination of Antibacterial Activities of Stinging Nettle (Urtica dioica) Ethanol Extract at Different Bacterial Concentrations

Yıl 2022, Cilt: 11 Sayı: 4, 953 - 959, 31.12.2022

Barış Gülhan , Filiz Yangılar

https://doi.org/10.17798/bitlisfen.1113660

Cited By: 1

Öz

The study evaluated the antibacterial effect of stinging nettle extract by the liquid microdilution method to obtain quantitative results. Unlike other studies in the literature, MIC and MBC values showing bacteriostatic and bactericidal effects were investigated for four different bacterial concentrations. Six pathogen strains were studied, including Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Streptococcus pneumoniae ATCC 45615, Klebsiella pneumoniae ATCC 70063, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and different levels of antimicrobial effects were determined. While the extract does not affect the Klebsiella pneumoniae ATCC 70063 strain at any level, Pseudomonas aeruginosa ATCC 27853 in strains 1.5x105 and 1.5x104 MIC values were found at concentrations, no effect was observed at the MBC level. While MIC and MBC values were found at the concentrations of Staphylococcus aureus ATCC 29213 except for the 1.5x107 concentration, the remaining standard strains had different MIC and MBC values at all concentrations. The standard strain Streptococcus pneumoniae ATCC 45615 was the most effective with the extract's lowest MIC and MBC values. This study showed that nettle extract (Urtica dioica) would contribute to the research results in the scientific literature on its antimicrobial effect.

Anahtar Kelimeler

Extraction, Antimicrobial effect, Nettle (Urtica dioica L.), Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC).

Kaynakça

• [1] A. F. Fathi, A. R. Garjani, N. Maleki, and D. S. Ranj, “Study of the hypoglycemic activity of the hydroalcoholic extract of Urtica dioica in normal and diabetic rats,” Pharmaceutical Sciences, vol. 2, pp. 65-69, 2005.
• [2] G. Vardatsikos, N. R. Pandey, and A. K. Srivastava, “Insulino-mimetic and anti-diabetic effects of zinc,” Journal of Inorganic Biochemistry, vol. 120, pp. 8-17, 2013
• [3] A. Bayrami, S. Haghgooie, S. R. Pouran, F. M. Arvanag, and A. Habibi-Yangjeh, “Synergistic antidiabetic activity of ZnO nanoparticles encompassed by Urtica dioica extract,” Advanced Powder Technology, vol. 31, pp. 2110-2118, 2020.
• [4] S. Zaman, “The medicinal plants. 6th ed. Tehran; Naghsh Press”; pp. 9-10, 2005.
• [5] M. Ramtin, A. Massiha, K. P. M. R. MAJID, K. Issazadeh, M. Assmar, and S. Zarrabi, “In vitro antimicrobial activity of Iris pseudacorus and Urtica dioica,” Zahedan Journal of Research in Medical Sciences, vol. 16, pp. 35-39, 2014.
• [6] N. Chaurasia, and M.Wichtl, “Sterols and steryl glycosides from Urtica dioica,” Journal of Natural Products, vol. 50, pp. 881-885, 1987.
• [7] M. Beschia, A. Leonte, and I. Oancea, “Phenolic components with biological activity in vegetable extracts,” Bulletin of the University of Galati, vol. 6, pp. 59-63, 1982.
• [8] L. Grauso, B. de Falco, V. Lanzotti, and R. Motti, “Stinging nettle, Urtica dioica L.: Botanical, phytochemical and pharmacological overview,” Phytochemistry Reviews, vol. 19, pp. 1341-1377, 2020.
• [9] S. M. T. Gharibzahedi, H. Rostami, and S. Yousefi, “Formulation design and physicochemical stability characterization of nanoemulsions of nettle (Urtica dioica) essential oil using a model‐based methodology,” Journal of Food Processing and Preservation, vol. 39, pp. 2947-2958, 2015.
• [10] C. Bourgeois, É. A. Leclerc, C. Corbin, J. Doussot, V. Serrano, J. R. Vanier, J. M. Seigneuret, D. Auguin, C. H. Pichon, É. Lainé, C. H. Hano, “Nettle (Urtica dioica L.) as a source of antioxidant and anti-aging phytochemicals for cosmetic applications,” Comptes Rendus Chimie, vol. 19, pp. 1090-1100, 2016.
• [11] M. Kačániová, K. Miklášová, S. Kunová, L. Galovičová, P. Borotová, V. Válková,... and M. Terentjeva, “Antimicrobial and Antioxidant Activity of Black Elder, Stinging Nettle, Marigold and Ribwort Plantain,” Scientific Papers: Animal Science & Biotechnologies/Lucrari Stiintifice: Zootehnie si Biotehnologii, vol. 54, 2021.
• [12] M. Ghaedi, R. Naghiha, R. Jannesar, N. Dehghanian, B. Mirtamizdoust, V. Pezeshkpour,. “Antibacterial and antifungal activity of flower extracts of Urtica dioica, Chamaemelum nobile and Salvia officinalis: Effects of Zn[OH]2 nanoparticles and Hp-2-minh on their property,” Journal of Industrial and Engineering Chemistry, vol. 32, pp. 353-359, 2015.
• [13] M. H. AlShuwayeb, and A. J. Al-Khatib, “Molecular and chemical therapeutic features of Urtica species,” European Scientific Journal, vol. 9, 2013.
• [14] R. Dhouibi, H. Affes, M. B. Salem, S. Hammami, Z. Sahnoun, K. M. Zeghal, and K. Ksouda, “Screening of pharmacological uses of Urtica dioica and others benefits,” Progress in Biophysics and Molecular Biology, vol. 150, pp. 67-77, 2020.
• [15] M. Holopainen, L. Jabordar, T. Seppanen-Laukso, I. Laakso, V. Kauppinen, “Antimicrobial Activity of Some Finnish Ericaceous plants,” Acta Pharmaceutica Fennica, vol. 97, pp. 197-202, 1988.
• [16] I. Gülçin, Ö. İ. Küfrevioǧlu, M. Oktay, and M. E. Büyükokuroǧlu, “Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.),” Journal of Ethnopharmacology, vol. 90, pp. 205-215, 2004.
• [17] S. A. Dar, F. A. Ganai, A. R. Yousuf, M. U. H. Balkhi, T. M. Bhat, and P. Sharma, “Pharmacological and toxicological evaluation of Urtica dioica,” Pharmaceutical Biology, vol. 51, pp. 170-180, 2013.
• [18] Z. Z. Kukrić, L. N. Topalić-Trivunović, B. M. Kukavica, S. B. Matoš, S. S. Pavičić, M. M. Boroja, and A. V. Savić, “Characterization of antioxidant and antimicrobial activities of nettle leaves (Urtica dioica L.),” Acta Periodica Technologica, vol.43, pp. 257-272, 2012.
• [19] A. Modarresi-Chahardehi, D. Ibrahim, S. Fariza-Sulaiman, and L. Mousavi, “Screening antimicrobial activity of various extracts of Urtica dioica,” Revista de Biologia Tropical, vol. 60, pp. 1567-1576, 2012.
• [20] K. K. Ghaima, N. M. Hashim, and S. A. Ali, “Antibacterial and antioxidant activities of ethyl acetate extract of nettle (Urtica dioica) and dandelion (Taraxacum officinale),” Journal of Applied Pharmaceutical Science, vol. 3, p. 96, 2013.
• [21] D. B. Külcü, C. D. Gökışık, and S. Aydın, “An investigation of antibacterial and antioxidant activity of nettle (Urtica dioica L.), mint (Mentha piperita), thyme (Thyme serpyllum) and Chenopodium album L. plants from Yaylacık Plateau, Giresun, Turkey,” Turkish Journal of Agriculture-Food Science and Technology, vol. 7, pp. 73-80, 2019.
• [22] S. Çolak, N. Çömlekcioğlu, and A. Aygan, “Investigation of antioxidant and antimicrobial activities of Urtica dioica L. plant extracts,” Eurasian Journal of Biological and Chemical Sciences, vol. 3, pp. 206-212, 202