Yeni Nesil Dizileme Teknolojilerinin Mikovirolojide Uygulanması

Yıl 2021, Cilt: 35 Sayı: 1, 247 - 263, 01.06.2021

Sahra Hosseınalızadeh , Serap Açıkgöz

Öz

Yeni nesil yüksek verimli DNA dizileme analizleri 21. yüzyılın başlarında kullanılabilir hale gelmiştir. Bu
dizileme teknolojisi genom karakterizasyonu, metagenetik, metilasyon analizi, kromatinlerin analizi,
mRNA'ların profillenmesi gibi birçok amaç için kullanılmaktadır. 2009 yılından sonra, yeni nesil dizileme
(YND) teknolojileri, virüs /viroid genom dizilemesi, keşfi ve tanısı, ekoloji ve epidemiyoloji, replikasyon ve
transkripsiyon dahil olmak üzere çeşitli bitki virüslerinde uygulanmıştır. Son yıllarda bu teknoloji sayesinde
araştırıcılar birçok yeni mikovirüsün tanılanmasını yapmışlardır. Bu derlemede, bazı yeni mikovirüslerin
karakterizasyonu ve tanılanmasında yeni nesil dizileme teknolojisi (YND) nin kullanılmasıyla ilgili konular ele
alınmıştır.

Anahtar Kelimeler

DNA dizileme, Virüs, Mikovirüs

Application of Next Generation Sequencing Technologies in Mycovirology

Öz

High-throughput next generation DNA sequencing analysis became available at the onset of the 21st
century. The sequencing technologies offer novel and rapid ways for genome-wide characterization and profiling of mRNAs, small RNAs, transcription factor regions, structure of chromatin and DNA methylation patterns, microbiology and metagenomics. After 2009, new generation sequencing (YND) technologies have been applied in various plant virology, including virus/viroid genome sequencing, discovery and diagnosis, ecology and epidemiology, replication and transcription. In recent years, researchers have identified many new mycoviruses through this technology. In this review, topics related to the use of new generation sequencing technology (YND) in characterization and identification of some new mycoviruses are discussed.

Anahtar Kelimeler

DNA sequencing, Virus, Mycovirus

Kaynakça

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