Swelling and Anticancer Drug Uptake Capacity of Chitosan/Hyaluronic Acid/Gelatin Hydrogels

Yıl 2024, Cilt: 7 Sayı: 1, 21 - 26, 31.07.2024

Betül Taşdelen , Ömer Faruk Güder

https://doi.org/10.55581/ejeas.1447096

Öz

Nowadays, the number of patients diagnosed with cancer is increasing day by day. Chemotherapy is an indispensable treatment method in many cancer treatments. Hydrogels have the ability to absorb/desorb water thanks to their cross-links and 3-dimensional network structures. In order to eliminate the disadvantages of chemotherapy applications and to ensure that a sufficient dose of drug is given to the patient, targeted drug release technologies have been developed and hydrogels have come to the fore in this field. Natural polymeric hydrogels are widely used in the medical field. Chitosan(CS) natural biopolymer has been used in controlled drug release systems for many years due to its unique properties such as high biocompatibility, biodegradability and low toxicity. By using different natural biopolymers with CS, the swelling and drup uptake capacity properties of the hydrogels can be improved. In this article, the swelling and drug uptake capacity of CS, CS/HA and CS/HA/GEL hydrogels in the 5-Fluorouracil (5-FU) used as a model drug was evaluated and drug uptake capacities were determined by UV-Vis spectrophotometer. As a result, swelling and drug uptake capacities of CS, CS/HA and CS/HA/GEL hydrogels in anticancer drug solution were investigated. While the 5-FU retention capacity (1.2 mg 5-FU/g dry gel) increased with the addition of HA to CS, a decrease in 5-FU retention capacity (0.4 mg 5-FU/g dry gel) was observed with the addition of GEL to CS. All the prepared hydrogels are promising in future for controlled drug delivery sytems in biomedical applications.

Anahtar Kelimeler

Hydrogel, Hyaluronic acid, Drug retention, Gelatin, Chitosan, 5-FU

Kitosan/Hiyaluronik Asit/Jelatin Hidrojellerinin Antikanser İlacında Şişme Davranışı ve İlaç Tutma Kapasitesinin İncelenmesi

Öz

Günümüzde kanser teşhisi konulan hasta sayısı her geçen gün artmaktadır. Pek çok kanser tedavisinde kemoterapi vazgeçilmez tedavi yöntemidir. Kemoterapi uygulamalarının dezavantajlarını ortadan kaldırmak ve hastaya yeterli dozda ilaç verilmesinin sağlanması amacıyla hedefe yönelik ilaç salım teknolojileri geliştirilmiş ve hidrojeller bu alanda öne çıkmıştır. Hidrojeller, çapraz bağları ve 3-boyutlu ağ yapıları sayesinde su absorplama ve desorplama yeteneğine sahiptirler. Doğal polimerik hidrojeller tıp alanında yaygın olarak kullanılmaktadır. Kitosan(CS) doğal biyopolimeri, yüksek biyouyumluluk, biyobozunabilirlik ve düşük toksisite gibi benzersiz özellikleri nedeniyle uzun yıllardır kontrollü ilaç salım sistemlerinde kullanılmaktadır. Farklı doğal biyopolimerlerin CS ile sentezlenmesi ile oluşan hidrojellerin şişme ve ilaç tutma kapasitesi özellikleri geliştirilebilir. Bu makalede, CS’ye hyaluronik asit(HA) ve jelatin(GEL) ilave edilmesiyle geliştirilmiş yeni tip CS/HA/GEL hidrojellerinin model ilaç olarak kullanılan 5-Fluorourasil(5-FU) antikanser ilacındaki kütlece şişmeleri değerlendirilmiş, UV-Vis spektrofotometre ile 5-FU ilacı tutma kapasiteleri belirlenmiştir. Sonuç olarak, CS, CS/HA ve CS/HA/GEL hidrojellerinin antikanser ilacında şişme davranışı ve ilaç tutma kapasiteleri incelendi. CS’ye HA eklenmesiyle 5-FU tutma kapasitesi (1.2 mg 5-FU/g kuru jel) artarken CS’ye GEL ilave edilmesiyle ise 5-FU tutma kapasitesi (0.4 mg 5-FU/g kuru jel) azalma gözlenmiştir. Sentezlenen hidrojeller, biyomedikal alanda kontrollü ilaç salım sistemlerinde umut vadetmektedir.

Anahtar Kelimeler

Hidrojel, Hyaluronik asit, İlaç Tutma, Jelatin, Kitosan, 5-FU

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