Diagnosis of Avian Tuberculosis in laying hens by pathological, microbiological and polymerase chain reaction (PCR): Case report

Yıl 2021, Cilt: 6 Sayı: 3, 312 - 317, 31.12.2021

Orhan Yavuz , Özgür Özdemir , Zafer Sayın , Fatih Hatipoğlu , Hasan Hüseyin Hadimli

https://doi.org/10.31797/vetbio.935334

Cited By: 1

Öz

Avian tuberculosis was described at 50 week-old Lohmann chickens in a commercial chicken farm. The study materials were 50 week-old 20 pieces of chicken from 2 coops and 16 week-old 20 pieces of pullets from 4 coops in a commercial chicken farm. After necropsy, samples were processed routinely for histopathological and microbiological examinations.
Macroscopically, hard consistency, numerous and different sizes, whitish-yellow caseificated-calcifiated nodules were seen on the liver, spleen, kidneys and intestinal serosa. At microscopic examination, various sized granulomas, which have been caseification necrosis surrounded by epithelioid histiocytes and multinucleated giant cells, were found at liver, spleen, wall and serosa of intestine. Numerous acid-fast bacteria were seen on histopathology at necrosis and macrophages in the liver, spleen and intestines by Ziehl – Neelsen staining. Mycobacterium avium spp was produced at microbiological inoculations in liver, spleen, intestines and ovaries. Mycobacterium avium subsp. avium from these cultures was identified by PCR.
It was thought that infection could be by fecal-oral route due to both intestinal tuberculosis in hens and common disease in coop. Therefore, the role of chicken manure may also be taken into account for the spreading of the disease.

Anahtar Kelimeler

Hens, Mycobacteriosis, Pathology, PCR

Kaynakça

• Ambrosio, S.R., Márcia De Deus, O.E., Rodriguez, C.A.R., Neto, J.S.F., & Amaku, M. (2008). Comparison of three decontamination methods for Mycobacterium bovis isolation. Brazilian Journal of Microbiology, 39, 241-4.
• Bolfion, M., Salehi, M., Ashrafi, H.J., Soleimani, K., Keshavarz, R., Aref, P.R., Mohammad, T.M., Tadayon, K., & Mosavari, N. (2010). Outbreak of avian Mycobacteriosis in flocks of domestic pigeons: an epidemiological approach. Iranian Journal of Microbiology, 2, 189-93.
• Coelho, A.C., Pinto, M.L., Matos, A., Matos, M., & Pires, M.A. (2013). Mycobacterium avium complex in domestic and wild animals. In: R.P. Carreira (Eds). Insights from Veterinary Medicine (pp. 91-128). Rijeka: InTech Publishing. https://doi.org/10.5772/56196
• Cromie, R.L., Brown, M.C., Forbes, N.A., Morgan, J., & Stanford, J.L. (1993). A comparison and evaluation of techniques for diagnosis of Avian Tuberculosis in wildfowl. Avian Pathology, 22, 617-30.
• Fulton, M.R., & Thoen, C.O. (2003). Tuberculosis. In: Y.M. Saif, H.J. Barnes, J.R. Glisson, A.M. Fadly, L.R. Mcdougald, D.E. Swayne (Eds). Diseases of poultry. 11th ed. (pp. 836-844). Iowa State University Press.
• Gonzalez, M., Rodriguez, A.B., Gimeno, I., Flores, J.M., & Pizarro M. (2002). Outbreak of avian Tuberculosis in 48 week-old commercial layer hen flock. Avian Disease, 46, 1055-61.
• Gümüşsoy, K.S., Beyaz, L., Aydin, F., Özcan, M., & Atasever A. (2006). Avian Tuberculosis in Kayseri zoo. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 3, 25-8.
• Hejlicek, K., & Treml F. (1995). Comparison of pathogenesis and epizootiology signification of avian mycobacteriosis in different sorts of domestic and free living synanthropic fowl. Veterinární Medicína, 40, 187–94.
• Jordan, F.T.W., & Hampson, D.J. (2008). Mycobacterium Avium – Avian Tuberculosis. In: M. Pattison, P.F. Mcmullin, D.J. Alexander (Eds). Poultry disease. 8th ed. (pp. 250-254). Saunders Elsevier Press.
• Kapakin, K.A.T., & Alçiğir G. (2009). The case of Tuberculosis in a pigeon. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 15, 477-9.
• Keymer, I.F., Jones, D.M., Pugsley, S.L., Wadsworth P.F. (1982). A survey of tuberculosis in birds in the regent's park gardens of the zoological society of London. Avian Pathology, 11, 563-9.
• Kriz, P., Slana, I., Mrlik, V., Moravkova, M., Kralova, A., Krizova, K., & Pavlik I. (2010). Mycobacterium avium subsp. avium in domestic pigeons (Columba livia f. domestica) diagnosed by direct conventional multiplex PCR: a case report. Veterinární Medicína, 55, 87-90.
• Kul, O., Tunca, R., Hazıroğlu, R., Diker, K.S., & Karahan S. (2005). An outbreak of avian Tuberculosis in peafowl (Pavo cristatus) and pheasants (Phasianus colchicus) in a zoological aviary in Turkey. Veterinární Medicína, 50, 446-50.
• Kunze, Z.M., Wall, S., Appelberg, R., Silva, M.T., Portaels, F., & Mcfadden J.J. (1991). IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium. Molecular Microbiology, 9, 2265–72.
• Kutsal, O., & Sağlam M. (1988). Güvercinlerde tüberküloz olgularının değerlendirilmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 35, 545-52.
• Mayahi, M., Mosavari, N., Esmaeilzadeh, S., & Asadollahi K.P. (2013). Avian Tuberculosis in naturally infected lofts of domestic pigeons, isolation, molecular identification and study of necropsy findings. The International Journal of Applied Research in Veterinary Medicine, 11, 194-201.
• OIE. (2014, December 20). Avian Tuberculosis. OIE Terrestrial Manual 2014. Chapter 2.3.6. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.06_AVIAN_TB.pdf
• Prukner-Radovcic, E., Culjak, K., Sostaric, B., Mazija, H., & Sabocanec R. (1998). Generalized Tuberculosis in pheasants at a commercial breeding farm. European Journal of Wildlife Research, 44, 33-9.
• Saggese, M.D., Riggs, G., Tizard, I., Bratton, G., Taylor, R., & Phalen D.N. (2007). Gross and microscopic findings and investigation of the aetiopathogenesis of mycobacteriosis in a captive population of white-winged ducks (Cairina scutulata). Avian Pathology, 36, 415-22.
• Sezen, Y., Erer, H., & Erganiş O. (1986). Bir güvercinde tüberküloz olgusu. Eurasian Journal of Veterinary Science, 2, 163-6.
• Shitaye, J.E., Matlova, L., Horvathova, A., Moravkova, M., Dvorska, L., Trcka, I., Lamka, J., Treml, F., Vrbas, V., & Pavlik I. (2008). Diagnostic testing of different stages of avian Tuberculosis in naturally infected hens (Gallus domesticus) by the tuberculin skin and rapid agglutination tests, faecal and egg examinations. Veterinární Medicína, 53, 101-10.
• Sousa, E., Momo, C., Costa, T.P., Pinheiro, S., & Werther K. (2008). Disseminated avian Tuberculosis in captive Ara macao. Brazilian Journal of Veterinary Pathology, 1, 28-31.