Antibacterial Effect of Bacteriocinogenic Enterococci from Different Sources on Listeria monocytogenes

Year 2020, Volume: 26 Issue: 1, 1 - 7, 05.03.2020

Emel Ünal Turhan , Zerrin Erginkaya , Alin Bayram Türemiş Mevhibe Terkuran

https://doi.org/10.15832/ankutbd.448475

Cited By: 1

Abstract

In this study, antimicrobial activity of partially purified enterocins and crude bacteriocins from Enterococcus isolates with different sources was investigated against Listeria monocytogenes by disk diffusion assay. Totally 70% of enterococcal isolates (Enterococcus faecalis and Enterococcus faecium from food and clinical sources) were found as potential bacteriocinogenic strains. Both of food and clinical enterococcal isolates also exhibited antimicrobial properties against L. monocytogenes. Additionally, the present study detected that inhibitory activity was strain-specific. Both crude bacteriocins and partially purified enterocins from E. faecium isolates showed lower antimicrobial activity against L. monocytogenes than E.faecalis isolates. The inhibition diameters obtained withcrude enterocins and partially purified enterocins were respectively ranging from 12.33 mm to 13.25 mm and from 8.66 mm to 9.25 mm. Crude bacteriocins retained antibacterial activity after heat treatment except 120 ^oC and also remained functional at pH values between 3 and 11. Asa result, it was considered that enterocins could be benefit in heated and acidic or basic food products as biopreservative.

Keywords

Bacteriocin; Enterocin; Enterococcus spp.; Listeria monocytogenes

References

• Anandani J H & Khan Z H (2014). Isolation, partial purification and biochemical characterization of enterocin producing enterococci. International Journal of Advanced Research 4(2):18-20.
• Aymerich T, Garriga M, Ylla J, Vallier J, Monfort J M & Hugas M (2000). Application of enterocins as biopreservatives against Listeria innocua in meat products. Journal of Food Protection 63(6):721-726.
• Bilgin H (2008). Antibacterial activity of a bacteriocinogenic bacterium isolated from a fermented milk product. Master thesis, Gaziosmanpaşa University, Tokat, Turkey.
• Campos C A, Rodriguez O, Calo-Mata P, Prado M & Barros-Velazquez J (2006). Preliminary characterization of bacteriocins from Lactococcuslactis, Enterococcus faecium and Enterococcus mundtiistrains isolated from turbot (Psettamaxima). Food Research International 39: 356-364.
• De Vuyst L, Morenoa M R F & Revets H (2003). Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins. International Journal of Food Microbiology 84: 299-318.
• Ennahar S & Deschamps N (2000). Anti-Listeria effect of enterocin A, produced by cheese-isolated Enterococcus faecium EFM01, relative to other bacteriocins from lactic acid bacteria. Journal of Applied Microbiology 88: 449-457.
• Franz C M A P, Toit M D, Holy A, Schillinger U & Holzapfel W H (1997). Production of nisin-like bacteriocins by Lactococcus lactis strains isolated from vegetables. Journal of Basic Microbiology 37(3): 187-196.
• Ghrairi T, Free J, Berjeaud J M & Manai M (2008). Purification and characterization of bacteriocin produced by Enterococcus faecium from Tunisian Rigouta Cheese. Food Control 19: 162-169.
• Gómez N C, Abriouel H, Grande M J, Pulido R P & Gálvez A (2012). Effect of enterocin AS-8 in combination with biocides on planktonic and sessile Listeria monocytogenes. Food Microbiology 30:51-58.
• Javed I, Ahmed S, Manam S, Riaz M, Ahmad B, Ali M I, Hameed A & Chaudry G J (2010). Production, characterization, and antibacterial activity of a bacteriocin from newly isolated Enterococcus faecium IJ-31. Journal of Food Protection 73(1): 44-52.
• Khalkhali S & Mojgani N (2017). Bacteriocinogenic potential and virulence traits of Enterococcus faecium and E. faecalis isolated from human milk. International Journal of Microbiology 9(4): 224-233.
• Klibi N, Jouini A, Rojo-Bezares B, Masmoudi A, Ruiz-Larrea F, Boudabous A & Torres C (2008). Phenotypic and genotypic characterization of bacteriocins in clinical enterococcal isolates of Tunisia. World Journal of Microbiology and Biotechnology 24: 653-657.
• Line J E, Svetoch E A, Eruslanov B V, Perelygin V V, Mitsevich E V, Mitsevich I P, Levchuk V P, Svetoch O E, Seal B S, Siragusa G R & Stern N J (2008). Isolation and purification of enterocin e-760 with broad antibacterial activity against gram-positive and gram-negative bacteria. Antibacterial Agents Chromatography 52(3): 1094-110.
• Marekova M, Laukova A, De Vuyst L, Skaugen M & Nes I F (2003). Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. Journal of Applied Microbiology 94: 523-530.
• Meera N S & Devi M C (2012). Partial characterization and optimization of parameters for bacteriocin production by probiotic lactic acid bacteria. Journal of Microbiology and Biotechnology Research 2(2): 357-365.
• Moreno M R F, Callewaert R, Devreese B, Van Beeumen J & De Vuyst L (2003). Isolation and biochemical characterization of enterocins produced by enterococci from different sources. Journal of Applied Microbiology 94: 214-229.
• Nami Y, Haghshenas B, Haghshenas M & Khosroushahi A Y (2015). Antibacterial activity and the presence of virulence factors and bacteriocin structural genes in Enterococcus faecium CM33 isolated from ewe colostrum. Frontier Microbiology 6(782): 1-10.
• Nascimento M S, Moreno I & Kuaye A Y (2010). Antibacterial activity of Enterococcus faecium fair-E 198 against Gram-Positive pathogens. Brazilian Journal of Microbiology 41: 74-81.
• Ogaki M B, Rocha K R, Terra M R, Furlaneto M C & Maia L F (2016). Screening of the enterocin-encoding genes and antibacterial activity in Enterococcus species. Journal of Microbiology and Biotechnology 26(6): 1026-1034.
• Özdemir G B, Oryaşın E, Bıyık H H, Özteber M & Bozdoğan B (2011). Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources. Indian Journal of Microbiology 51(2): 182-187.
• Savadago A, Ouattara C A T, Bassole I H N & Traore A S (2004). Antibacterial activities of lactic acid bacteria strains isolated from Burkinofaso fermented milk. Pakistan Journal of Nutrition 3(3): 174-179.
• Theppangna W, Murase T, Tokumaru N, Chikumi H, Shimizu E & Otsuki K (2007). Screening of the enterocin genes and antibacterial activity against pathogenic bacteria in enterococcus strains obtained from different origins. Journal of Veterinary Medicine Science 69(12):1235-1239.
• Uymaz B (2009). Isolation and identification of probiotic lactobacilli originated from food and human and characterization of the ability of bacteriocin production. PhD Thesis, Ankara Üniversitesi, Ankara, Turkey.
• Vijayakumar P P & Muriana P M (2017). Inhibition of Listeria monocytogenes on ready-to-eatmeats using bacteriocin mixtures based on mode-of-action. Foods 6(22): 1-13.
• Vimont A, Fernandez B, Hammami R, Ababsa A, Daba H & Fliss I (2017). Bacteriocin-producing Enterococcus faecium lcw44:a high potential probiotic candidate from raw camel milk. Frontier Microbiology 8(865): 1-8.
• Yamato M, Ozaki K & Ota F (2003). Partial purification and characterization of the bacteriocin produced by Lactobacillus acidophilus YIT0154. Microbiology Research 158: 169-172.
• Yildirim Z, İlk Y, Yildirim M, Tokatli K & Öncül N (2014). Inhibitory effect of enterocin KP in combination with sublethal factors on Escherichia coli O157:H7 or Salmonella Typhimurium in BHI broth and UHT milk. Turkish Journal Biology 38: 412-419.
• Zhou F, Zhao H, Bai F, Dziugan P, Liu Y & Zhang B (2014). Purification and characterization of the bacteriocin produced by Lactobacillus plantarum, isolated from Chinese pickle. Czech Journal Food Science 32(5): 430-436.
• Zheng W, Zhang Y, Lu H M, Li D T, Zhang Z L, Tang Z X & Shi L E (2015). Antibacterial activity and safety evaluation of KQ2.6 isolated from Peacock feces. BMC Biotechnology 15(30): 1-8.