Identification of bioactive peptides in kashar cheese and its antioxidant activities

Year 2018, Volume: 2 Issue: 2, 44 - 49, 10.04.2018

Zübeyde Öner , Ayşe Mine Sarıdağ

https://doi.org/10.31015/jaefs.18007

Cited By: 4

Abstract

This study analyzed the peptide
profile and antioxidant activity in commercially sourced Kashar cheese. The
antioxidant activity of Kashar cheese was found to be 41.09 mM Trolox g−1.
However, the antioxidant activity of its F3, F4, F5 and F14 fractions was found
to be 920.726  mM  Trolox g−1,  545.544 mM  Trolox g−1 ,  783.864 mM Trolox g−1, 
and 392.12 mM Trolox g−1, respectively. In Kashar cheese, the Tandem Mass Spectrometry
(MS/MS) spectrum for the 875 g mol−1 m/z signal was matched to αs1-casein, and
it showed that 1012 g mol−1 (875+137) histidine can be a part of 1140
(1012+128) glutamine amino acid. Peptide sequences were matched to 875:RPKHPIK-H-Q
peptide 1012:RPKHPIK+H peptide and 1140:RPKHPIKH+Q peptide. It can be concluded
that the peptide fractions of Kashar cheese demonstrated antioxidant activity.

Keywords

Bioactive peptides, Kashar cheese, Antioxidant activity

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