Altınbaş Kefal (Chelon auratus) Pullarından Asitte Çözünür Kollajen Ekstraksiyonu ve Karakterizasyonu

Year 2023, Volume: 6 Issue: 2, 166 - 177, 27.12.2023

Cansu Metin Hacisa , Taçnur Baygar

https://doi.org/10.46384/jmsf.1387324

Abstract

Bu çalışmada değerli bir biyoaktif materyal elde etmek amacıyla atık balık pullarının potensiyali incelenmiştir. Altınbaş kefal (Chelon auratus) pullarından asitte çözünür kollajen izole edilerek karakterize edilmiştir. Ekstrakte edilen kollajenin besin kompozisyonu, denatürasyon sıcaklığı, Sodyum dodesil sülfat jel elektroforezi (SDS-PAGE), amino asit bileşimi, Taramalı elektron mikroskobu ve Enerji dağılımlı X‑ışınları spektrometre (SEM-EDS), Fourier dönüşümü kızılötesi spektrofotometre (FTIR), Termogravimetrik analiz (TGA), antioksidan ve antimikrobiyal aktivite analizleri yapılarak ticari kollajenle karşılaştırılmıştır. Sonuç olarak, balık pulu atıklarından, doğal atıkların veya çevre sorunlarının yönetimine katkı sağlayacak, memeli kollajenine alternatif, gıda ve kozmetik gibi endüstriyel alanlarda kullanılabilecek bir kaynak elde edilmiştir.

Keywords

Biyoaktif madde, Asitte çözünür kollajen, Balık pulu, Altınbaş kefal, Kollajen karakterizasyonu

Extraction and Characterization of Acid Soluble Collagen From Golden Grey Mullet (Chelon auratus) Scale

Abstract

In the present study, the potential of waste fish scales for obtaining a valuable bioactive material was evaluated. Acid soluble collagen from golden grey mullet (Chelon auratus) scales was isolated and characterized successfully. Proximate composition, denaturation temperature, Sodium dodecyl sulphate gel electrophoresis (SDS-PAGE), amino acid composition, Scanning electron microscopy and Energy dispersive X‑ray spectroscopy (SEM-EDS), Fourier transform infrared spectrophotometer (FTIR), Thermogravimetric analysis (TGA), antioxidant and antimicrobial activity analyses were performed for the extraction of collagen and extracted collagen was compared with commercial collagen. As a result, an alternative and useful source for mammalian collagen for the industrial applications like food and cosmetics was extracted from the fish scale waste material, which may help mitigate the management of natural wastes or environmental problems.

Keywords

Bioactive substances, Acid soluable collagen, Fish scale, Golden grey mullet, Collagen characterization

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