Çeşitli gıda örnekleri ve kesimhanelerden izole edilen bazı patojen bakterilerin biyofilm oluşturma yeteneğinin araştırılması

Year 2022, Volume: 7 Issue: 3, 338 - 345, 31.12.2022

Tolga Uyanık , Ayşegül Bölükbaş , Ali Gücükoğlu , Özgür Çadırcı

https://doi.org/10.31797/vetbio.1194207

Cited By: 3

Abstract

Biyofilmler, bakterilere güçlü tolerans ve uygun yaşam ortamları sağlayan, ekstraselüler polimerik maddelere gömülü mikroorganizmalar topluluğudur. Çoğu patojen özellikteki mikroorganizma, uygun koşullar oluştuğunda gıdalarda ve gıda ile temas eden yüzeylerde biyofilm oluşturarak gıda endüstrisinde ve halk sağlığı açısından sorunlara yol açmaktadır. Bu çalışmada, daha önceki çalışmalarla çeşitli gıda örnekleri ve kesimhane ortamından izole edilen ve moleküler yöntemlerle identifiye edilmiş, toplam 120 Salmonella Virchow, Salmonella Infantis, Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium izolatı materyal olarak kullanıldı. Biyofilm oluşumunun kalitatif olarak tespiti amacıyla Kongo Kırmızısı Agar kullanıldı. Yapılan analizler doğrultusunda, toplam 120 izolattan 15’inin (%12,5) kalitatif olarak biyofilm ürettiği tespit edildi. Kalitatif olarak biyofilm oluşturduğu tespit edilen suşların biyofilm oluşturma gücü mikroplak yöntemiyle kantitatif olarak araştırıldı. Mikroplak yöntemine göre 2 E. faecium ve 1 E. faecalis suşu güçlü biyofilm üreticisi; 2 L. monocytogenes (serotip 1/2a) suşu orta düzey biyofilm üreticisi; 4 E. coli, 4 S. aureus ve 2 L. monocytogenes (serotip 1/2a ve serotip 4b) suşu zayıf biyofilm üreticisi olarak belirlendi. Salmonella spp. suşlarının tümü biyofilm üretimi açısından negatif olarak belirlendi. Sonuç olarak; süt, mandıra ürünleri, tüketime hazır gıdalar ve kesimhanelerden izole edilen bakterilerde biyofilm üretiminin hem halk sağlığı hem de gıda işletmeleri için önemli olduğu düşünülmektedir. Bu nedenle, gıda işleme tesislerinde, üretim hattı boyunca temizlik ve dezenfeksiyon işlemlerinin gerçekleştirilmesinde HACCP, İyi Üretim Uygulamaları gibi gıda sanitasyon sistemlerine riayet edilmesinin biofilm oluşumunun önüne geçilmesinde faydalı olacağı öngörülmektedir.

Keywords

Biyofilm, gıda, Enteroccocus, Escherichia coli, Listeria monocytogenes, Staphylococcus aureus

Evaluation of biofilm forming ability of some pathogenic bacteria Isolated from various food samples and slaughterhouses

Abstract

Biofilms are ensemble of microorganisms embedded in extracellular polymeric substances that providing bacteria strong tolerance and suitable habitats. In this study, a total of 120 Salmonella Virchow, Salmonella Infantis, Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium isolates, which were isolated from various food samples and slaughterhouse environment and identified by molecular methods, were used as material. Congo Red Agar was used for the qualitative detection of biofilm formation. According to results, it was determined that 15 (12,5%) out of 120 isolates produced biofilm qualitatively. Biofilm forming levels of qualitative biofilm producers were later quantitatively investigated by microplate method. According to the microplate method, 2 E. faecium and 1 E. faecalis strains are strong biofilm producers; 2 strains of L. monocytogenes (serotype 1/2a) are moderate biofilm producers; 4 strains of E. coli, 4 S. aureus and 2 L. monocytogenes (serotype 1/2a and serotype 4b) were determined as weak biofilm producers. All Salmonella spp. strains were negative for biofilm production. As a result; it is thought that biofilm production in bacteria isolated from milk, dairy products, ready-to-eat foods and slaughterhouses is important for both public health and food industry. It is anticipated that, in the cleaning and disinfection of food processing facilities, compliance with food sanitation systems such as HACCP and Good Manufacturing Practices will be beneficial to prevent biofilm formation.

Keywords

Biofilm, Enterococcus, Escherichia coli, food, Listeria monocytogenes, Staphylococcus aureus

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