INVESTIGATION OF SOME WATER SOLUBLE PARAMETERS IN APRICOT FRUIT OF DIFFERENT VARIETIES CULTIVATED

Yıl 2018, , 925 - 929, 01.11.2018

Ahmet Baysar , Fikret Karataş

https://doi.org/10.15237/gida.GD18084

Cited By: 1

Öz

In this study, the amounts of
reduced form of glutathione (GSH), oxidized form of glutathione (GSSG) and
various B vitamins particularly, thiamine hydrochloride (vitamin B1),
riboflavin (vitamin B2), nicotinic acid (vitamin B3), pyridoxine hydrochloride (vitamin
B6) and folic acid (vitamin B9) in fresh ripe apricot fruits of six different
varieties were determined by High Performance Liquid Chromatography(HPLC). It
was observed that GSH, GSSG, vitamin B1, vitamin B2, vitamin B3, vitamin B6 and
vitamin B9 levels were between 914.70±24.10 – 82.56±5.92 µg/g; 35.28±2.73 –
17.16±2.05 µg/g, 0.49±0.08 - 1.02±0.13 µg/g, 1.56±0.14 - 4.48±0.46 µg/g,
16.10±2.46 5.40±0.52 µg/g, 23.85±2.90 - 7.86±0.58 µg/g and 8.84±0.86 -
3.04±0.29 µg/g, respectively. The Hudayı variety was rich in GSH, GSGH, vitamin
B1 and B2, Kabaaşı variety was rich in Vitamin B3 and B6 and Hacıhaliloğlu
variety was rich in vitamin B9.

Anahtar Kelimeler

Apricot, glutathione, vitamins B, HPLC

FARKLI KAYISI ÇEŞİTLERİNDE SUDA ÇÖZÜNÜR BAZI PARAMETRELERİN ARAŞTIRILMASI

Öz

Bu çalışmada taze ve olgun altı farklı kayısı çeşidinin
redükte glutatyon (GSH), okside glutatyon (GSSG) ile çeşitli B vitamini
özellikle tiamin hidroklorür (B1), riboflavin (B2), Nikotinik asit (B3)
piridoksin hidroklorür (B6) ve folik asit (B9) içerikleri Yüksek Performanslı
Sıvı Kromatografisi (HPLC) kullanılarak belirlenmiştir. GSH, GSSG, B1, B2, B3,
B6 ve B9 vitaminlerinin içeriği sırasıyla 914.70±24.10 – 82.56±5.92 µg/g;
35.28±2.73 – 17.16±2.05 mg/g, 0.49±0.08 - 1.02±0.13 µg.g-1, 1.56±0.14 -
4.48±0.46 µg.g-1, 16.10±2.46 5.40±0.52 µg/g, 23.85±2.90 - 7.86±0.58 µg/g ve
8.84±0.86 - 3.04±0.29 µg/g arasında olduğu gözlenmiştir. Hudayı çeşidinin GSH,
GSSG, B1 ve B2 vitamini, Kabaaşı çeşidinin de B3 ve B6 vitaminleri ve
Hacıhaliloğlunun B9 vitamini bakımından zengin olduğu görülmüştür.

Anahtar Kelimeler

Kayısı, Glutatyon, B Vitaminleri, HPLC

Kaynakça

• Amidzic, R., Brboric, J., Cudina, O., Vladimirov, S. (2005). RP-HPLC Determination of vitamins B1, B3, B6, folic acid and B12 in multivitamin tablets. J Serb Chem Soc. 70(10): 1229-35.
• Asma, B.M. (2000) Apricot growing. Malatya Turkey: Evin Publishers (in Turkish).
• Belloso, M.O, Barriobero, L.E. (2001) Proximate composition, minerals and vitamins in selected canned vegetables. Eur Food Res Technol. 212: 182 -87.
• Berg, J.M., Tymazko, J.L., Stryer, L. (2002). Biochemistry, W.H. Freeman and Company, fifth edition, New York USA. pp. 340-47
• Combs, Jr G.F., (1992). Discovery of the vitamins, in: The Vitamins: Fundamental Aspects in Nutrition and Health, Academic Press, Inc., San Diego, CA, USA, p. 9.
• Dawes, P., Dawes, E. (2000). SGE Chromatography Products Catalog. p. 182.
• Ercisli, S., Esitken, H., Guleryuz, M. (1999). The effect of vitamines on the seed germination of apricots. International Symposıum on Apricot Culture, Vols 1 and 2. Book Series: Acta Hortıculturae, Issue: 488, pp: 437-440.
• Gundogdu, M., Kan, T., Gecer, M.K. (2013). Vitamins, flavonoids, and phenolic acid levels in early- and late-ripening apricot (prunus armeniaca L.) cultivars from Turkey. HortScience. 48 (6): 696-700.
• Hayes, J.D., McLellan, L.I. (1999). Glutathione and glutathione dependent enzymes represent a co-ordinately regulated defence against oxidative stress. Free Rad Res. 31: 273-300.
• Ivanovic, D., Popovic, A., Radulovic, D., Medenica, M. (1999). Reversed-phase ion-pair HPLC determination of some water-soluble vitamins in pharmaceuticals. J Pharm Biomed Anal. 18: 999-1004.
• Karatas, F., Öbek, E., Kamışlı, F. (2009). Antioxidant capacity of Lemna gibba L. exposed to wastewater treatment. J Ecol Eng. 35: 1225-30.
• Kocsy, G., Galiba, G., Brunold, C. (2001). Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiol Plantarum. 113: 158-64.
• Lee, S.K., Kader, A.A. (2000). Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biol Technol. 20: 207–220.
• Meister, A. (1994). Glutathione ascorbic acid antioxidant system in animals. J Biol Chem. 269(13): 9397-400.
• Noctor, G., Foyer, C.H. (1998). Ascorbate and glutathione: keeping active oxygen under control. Ann Rev Plant Physiol Plant Mol Biol. 49: 249–279.
• Pala, M., Ackurt, F., Loker M, Saygı, Y.B. (1994). Composition of apricot varieties. Standard; Special Apricot Issue: 64-6 6(in Turkish).
• Papadoyanni, I.N. (1990). HPLC in clinical chemistry. Marcel Dekker, Chapter 23, New York, USA. pp. 440–72.
• Paunovic, S. A. (1985). Apricot culture and apricot science. Acta Hortic., 192: 23–34.
• Sha,n X., Aw, T.Y., Jones, D.P. (1990). Glutathione-dependent protection against oxidative injury. Pharmac Ther. 47: 61-71.
• State Institute of Statistics (SIS) (1998). Agricultural structure and production number of fruit trees and fruit production. Ankara, Turkey (in Turkish).
• Steven, H.Z. (2000). Dietary References Intakes for Thiamine, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Panthotenic Acid, Biotin and Choline, Institute of Nutrition, National Academy Press.
• Tausz, M., Sircelj, H., Grill, D. (2004). The glutathione system as a stress marker in plant ecophysiology: is a stress-response concept valid? J Exp. Bot. 55 (404), 1955–62.
• Uguralp, S,. Ozturk, F., Aktay, G., Cetin, A., Gursoy, S. (2012). The antioxidant effects of dry apricot in the various tissues of rats with induced cold restraint stress. Nat Prod Res. 26(16): 1535-38.
• Uslu, S., Genç, Ç., Bilici, M., Bilici, A., Doganay, S. 1995. Nutrient status of apricot orchards in Malatya. Acta Hortic. 384: 355-56.
• Watt, B.K., Merrill, A.L. (1978). Composition of Foods. Agriculture Handbook. No 8-3, US Department of Agriculture Science and Education Administration, Washington DC.