Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties

Yıl 2023, Cilt: 7 Sayı: 2, 399 - 407, 29.06.2023

Polat İpek , Mehmet Fırat Baran , Reşit Yıldız , Abdulkerim Hatipoğlu

https://doi.org/10.31015/jaefs.2023.2.18

Cited By: 3

Öz

This study was carried out to synthesize silver nanoparticles (AgNPs) from Arum dioscoridis (AD) leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible (UV-vis) Spectroscopy, Field Emission Scanning Electron Microscopy (FE-SEM), Electron Dispersive X-ray (EDX) Spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy, Transmission Electron Microscopy (TEM), X-ray Diffractometer (XRD), Atomic Force Microscopy (AFM) and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration (MIC) values (mg/L) of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid] assay, the half-maximal inhibitory concentrations (IC50: μg/mL) of AD-AgNPs on human colon adenocarcinoma cell (CACO-2), human breast cancer cell (MCF-7), glioblastoma multiforme cell (T98-G), and healthy human umbilical vein endothelial cell (HUVEC) lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.

Anahtar Kelimeler

AgNPs, Arum dioscoridis extract, Cytotoxicity, Green chemistry, Pathogen microorganism

Destekleyen Kurum

Dicle University Scientific Research Projects Coordinatorship

Proje Numarası

VETERINARY.21.002

Kaynakça

• Abdelkader, D. H., Negm, W. A., Elekhnawy, E., Eliwa, D., Aldosari, B. N., Almurshedi, A. S. (2022). Zinc Oxide Nanoparticles as Potential Delivery Carrier: Green Synthesis by Aspergillus niger Endophytic Fungus, Characterization, and In Vitro/In Vivo Antibacterial Activity. Pharmaceuticals, 15, 1057. https://doi.org/10.3390/ph15091057
• Aktepe, N., Baran, A. (2021). Fast and Low-Cost Biosynthesis of AgNPs with Almond Leaves: Medical Applications with Biocompatible Structures. Progress in Nutrition, 23(3), e2021271. https://doi.org/10.23751/pn.v23i3.11996
• Ali, I. A. M., Ahmed, A. B., Al-Ahmed, H. I. (2023). Green synthesis and characterization of silver nanoparticles for reducing the damage to sperm parameters in diabetic compared to metformin. Scientific Reports, 13, 2256. https://doi.org/10.1038/s41598-023-29412-3
• Alias Antonysamy, M. J., Santhanam, A., Thangaiah, S., Narayanan, J. (2017). Green synthesis of silver nanoparticles using Cyathea nilgirensis Holttum and their cytotoxic and phytotoxic potentials. Particulate Science and Technology, 36(5), 578-582. https://doi.org/10.1080/02726351.2016.1278292
• Alkhulaifi, M. M., Alshehri, J. H., Alwehaibi, M. A., Awad, M. A., Al-Enazi, N. M., Aldosari, N. S., Hatamleh, A. A., Abdel- Raouf, N. (2020). Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties. Saudi Journal of Biological Sciences, 27(12), 3434-3441. https://doi.org/10.1016/j.sjbs.2020.09.031
• Aryan, Ruby, Mehata, M. S. (2021). Green synthesis of silver nanoparticles using Kalanchoe pinnata leaves (life plant) and their antibacterial and photocatalytic activities. Chemical Physics Letters, 778, 138760. https://doi.org/10.1016/j.cplett.2021.138760
• Atalar, M. N., Baran, A., Hatipoğlu, A., Baran, M. F., Yavuz, Ö., Aktepe, N., Keskin, C. (2021). The Characterization of Silver Nanoparticles Synthesized from Prunus spinosa Fruit and Determination of Antimicrobial Effects on Some Food Pathogens. European Journal of Science and Technology, (32), 298-305. https://doi.org/10.31590/ejosat.1040082
• Baran, A., Baran, M. F., Keskin, C., Irtegun Kandemir, S., Valiyeva, M., Mehraliyeva, S., Khalilov, R., Eftekhari, A. (2021). Ecofriendly/Rapid Synthesis of Silver Nanoparticles Using Extract of Waste Parts of Artichoke (Cynara scolymus L.) and Evaluation of their Cytotoxic and Antibacterial Activities. Journal of Nanomaterials, 2021, 2270472. https://doi.org/10.1155/2021/2270472
• Baran, A., Baran, M. F., Keskin, C., Hatipoğlu, A., Yavuz, Ö., İrtegün Kandemir, S., Adican, M. T., Khalilov, R., Mammadova, A., Ahmadian, E., Rosic, G., Selakovic, D., Eftekhari, A. (2022). Investigation of antimicrobial and cytotoxic properties and specification of silver nanoparticles (AgNPs) derived from Cicer arietinum L. green leaf extract. Frontiers in Bioengineering and Biotechnology, 10, 855136. https://doi.org/10.3389/fbioe.2022.855136
• Baran, M. F., Keskin, C., Baran, A., Hatipoğlu, A., Yildiztekin, M., Küçükaydin, S., Kurt, K., Hoşgören, H., Sarker, M.M.R., Sufianov, A., Beylerli, O., Khalilov, R., Eftekhari, A. (2023). Green Synthesis of Silver Nanoparticles from Allium cepa L. Peel Extract, Their Antioxidant, Antipathogenic, and Anticholinesterase Activity. Molecules 28, 2310. https://doi.org/10.3390/molecules28052310
• Basavarajappa, D. S., Kumar, R. S., Almansour, A. I., Chakraborty, B., Bhat, M. P., Nagaraja, S. K., Hiremath, H., Perumal, K., Nayaka, S. (2022). Biofunctionalized silver nanoparticles synthesized from Passiflora vitifolia leaf extract and evaluation of its antimicrobial, antioxidant and anticancer activities. Biochemical Engineering Journal, 187, 108517. https://doi.org/10.1016/j.bej.2022.108517
• Çeçen, C., Akan, H. Balos, M. M. (2020). Anatomical and Morphological Investigation of Arum rupicola Boiss var. rupicola and Arum dioscoridis Sm. Taxa Naturally Distributed in Şanlıurfa Region. Journal of Agriculture and Nature, 23(1), 135-147. https://doi.org/10.18016/ksutarimdoga.vi.576432
• Chaudhary, R., Nawaz, K., Khan, A. K., Hano, C., Abbasi, B. H., Anjum, S. (2020). An Overview of the Algae-Mediated Biosynthesis of Nanoparticles and Their Biomedical Applications. Biomolecules, 10(11), 1498. https://doi.org/10.3390/biom10111498
• Debnath, G., Das, P., Saha, A. K. (2019). Green Synthesis of Silver Nanoparticles Using Mushroom Extract of Pleurotus giganteus: Characterization, Antimicrobial, and α-Amylase Inhibitory Activity. BioNanoScience, 9, 611-619. https://doi.org/10.1007/s12668-019-00650-y
• Dikshit, P. K., Kumar, J., Das, A. K., Sadhu, S., Sharma, S., Singh, S., Gupta, P. K., Kim, B. S. (2021). Green Synthesis of Metallic Nanoparticles: Applications and Limitations. Catalysts, 11, 902. https://doi.org/10.3390/catal11080902
• Dixit, D., Gangadharan, D., Popat, K. M., Reddy, C. R. K., Trivedi, M., Gadhavi, D. K. (2018). Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection. Water Science and Technology, 78(1), 235-246. https://doi.org/10.2166/wst.2018.292.
• Donga, S., Chanda, S. (2021). Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system). Artificial Cells, Nanomedicine, and Biotechnology, 49(1), 292-302. https://doi.org/10.1080/21691401.2021.1899193
• Fahmy, H. M., Mosleh, A. M., Elghany, A. A., Shams Eldin, E., Abu Sere, E. S., Alia, S. A., Shalan, A. E. (2019). Coated silver nanoparticles: synthesis, cytotoxicity, and optical properties. RSC Advances, 9(35), 20118-20136. https://doi.org/10.1039/C9RA02907A
• Farshori, N. Y., Al-Oqail, M. M., Al-Sheddi, E. S., Al-Massarani, S. M., Saquib, Q., Siddiqui, M. A., Wahab, R., Al-Khedhairy, A. A. (2022). Green synthesis of silver nanoparticles using Phoenix dactylifera seed extract and its anticancer effect against human lung adenocarcinoma cells. Journal of Drug Delivery Science and Technology, 70, 103260. https://doi.org/10.1016/j.jddst.2022.103260
• Garibo, D., Borbón-Nuñez, H. A., de León, J. N. D. Mendoza, E. G., Estrada, I., Toledano-Magaña, Y., Tiznado, H., Ovalle-Marroquin, M., Soto-Ramos, A. G., Blanco, A., Rodríguez, Romo, O. A., Chávez-Almazán, L. A., Susarrey-Arce, A. (2020). Green synthesis of silver nanoparticles using Lysiloma acapulcensis exhibit high-antimicrobial activity. Scientific Reports, 10, 12805. https://doi.org/10.1038/s41598-020-69606-7
• Gour, A., Jain, N. K. (2019). Advances in green synthesis of nanoparticles. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 844-851. https://doi.org/10.1080/21691401.2019.1577878
• Gur, T., Meydan, I., Seckin, H., Bekmezci, M., Sen, F. (2022). Green synthesis, characterization and bioactivity of biogenic zinc oxide nanoparticles. Environmental Research, 204, 111897. https://doi.org/10.1016/j.envres.2021.111897
• Hamida, R. S. Elsayed, N. E., M. Abdelaal Ali, M., Bin-Meferij, M. M., Khali, M. I. (2020). Synthesis of Silver Nanoparticles Using a Novel Cyanobacteria Desertifilum sp. extract: Their Antibacterial and Cytotoxicity Effects. International Journal of Nanomedicine,15, 49-63. https://doi.org/10.2147/IJN.S238575
• Hamouda, R. A., Hussein, M. H., Abo-elmagd Rasha A., Bawazir, S. S. (2019). Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica. Scientific Reports, 9(1), 1-17. https://doi.org/10.1038/s41598-019-49444-y
• Hatipoğlu, A. (2021a). Rapid green synthesis of gold nanoparticles: synthesis, characterization, and antimicrobial activities. Progress in Nutrition, 23(3), e2021242. https://doi.org/10.23751/pn.v23i3.11988
• Hatipoğlu, A. (2021b). Green synthesis of silver nanoparticles using Abelmoschus esculentus leaf and antimicrobial effects on some food pathogens. Artvin Coruh University Journal of Forestry Faculty, 22(2), 239-246. https://doi.org/10.17474/artvinofd.971246
• Hatipoğlu, A. (2022a). Green biosynthesis of silver nanoparticles using Prunus cerasifera pissardii nigra leaf and their antimicrobial activities against some food pathogens. Czech Journal of Food Science, 40, 383-391. https://doi.org/10.17221/156/2021-CJFS
• Hatipoğlu, A. (2022b). Green Synthesis of Silver Nanoparticles and Their Antimicrobial Effects on Some Food Pathogens. Süleyman Demirel University Journal of Natural and Applied Sciences, 26(1), 106-114. https://doi.org/10.19113/sdufenbed.970654
• Heuer-Jungemann, A., Feliu, N., Bakaimi, I., Hamaly, M., Alkilany, A., Chakraborty, I., Masood, A., Casula, M. F., Kostopoulou, A., Oh, E., Susumu, K., Stewart, M. H., Medintz, I. L., Stratakis, E., Parak, W. J., Kanaras, A. G. (2019). The Role of Ligands in the Chemical Synthesis and Applications of Inorganic Nanoparticles. Chemical Reviews, 119(8), 4819-4880. https://doi.org/10.1021/acs.chemrev.8b00733
• Irtegun Kandemir, S., Ipek, P. (2023). Antiproliferative effect of Potentilla fulgens on glioblastoma cancer cells through downregulation of Akt/mTOR signaling pathway. Journal of Cancer Research and Therapeutics, 0(0), 0. https://doi.org/10.4103/jcrt.jcrt_1886_21
• Jamkhande, P. G., Ghule, N. W., Bamer, A. H., Kalaskar, M. G. (2019). Metal nanoparticles synthesis: An overview on methods of preparation, advantages and disadvantages, and applications. Journal of Drug Delivery Science and Technology, 53, 101174. https://doi.org/10.1016/j.jddst.2019.101174
• Kanniah, P., Radhamani, J., Chelliah, P., Muthusamy, N., Thangapandi, E. J. J. S. B., Thangapandi, J. R., Balakrishnan, S., Shanmugam, R. (2020). Green Synthesis of Multifaceted Silver Nanoparticles Using the Flower Extract of Aerva lanata and Evaluation of Its Biological and Environmental Applications. ChemistrySelect, 5, 2322-2331. https://doi.org/10.1002/slct.201903228
• Keskin, C., Baran, A., Baran, M. F., Hatipoğlu, A., Adican, M. T., Atalar, M. N., Huseynova, I., Khalilov, R., Ahmadian, E., Yavuz, Ö., İrtegün Kandemir, S., Eftekhari, A. (2022). Green Synthesis, Characterization of Gold Nanomaterials using Gundelia tournefortii Leaf Extract, and Determination of Their Nanomedicinal (Antibacterial, Antifungal, and Cytotoxic) Potential. Journal of Nanomaterials, 2022, 7211066. https://doi.org/10.1155/2022/7211066
• Khan, M., Khan, A. U., Bogdanchikova, N., Garibo, D. (2021). Antibacterial and Antifungal Studies of Biosynthesized Silver Nanoparticles against Plant Parasitic Nematode Meloidogyne incognita, Plant Pathogens Ralstonia solanacearum and Fusarium oxysporum. Molecules, 26(9), 2462. https://doi.org/10.3390/molecules26092462
• Kozuharova, E., Naychov, Z., Kochmarov, V. Benbassat, N., Gibernau, M., Momekov, G. (2020). The potential of Arum spp. as a cure for hemorrhoids: chemistry, bioactivities, and application. Advances in Traditional Medicine, 20, 133-141. https://doi.org/10.1007/s13596-020-00425-x
• Lopes, C. R. B., Courrol, L. C. (2018). Green synthesis of silver nanoparticles with extract of Mimusops coriacea and light. Journal of Luminescence, 199: 183-187. https://doi.org/10.1016/j.jlumin.2018.03.030
• Mariadoss, A. V. A., Ramachandran, V., Shalini, V., Agilan, B., Franklin, J. H., Sanjay, K., Alaa, Y. G., Tawfiq, M. A., Ernest, D. (2019). Green synthesis, characterization and antibacterial activity of silver nanoparticles by Malus domestica and its cytotoxic effect on (MCF-7) cell line. Microbial Pathogenesis, 135, 103609. https://doi.org/10.1016/j.micpath.2019.103609
• Naghmachi, M., Raissi, A., Baziyar, P., Homayoonfar, F., Amirmahani, F., Danaei, M. (2022). Green synthesis of silver nanoparticles (AgNPs) by Pistacia terebinthus extract: Comprehensive evaluation of antimicrobial, antioxidant and anticancer effects. Biochemical and Biophysical Research Communications, 608, 163-169. https://doi.org/10.1016/j.bbrc.2022.04.003
• Naveed, M., Batool, H., Rehman, S. U., Javed, A., Makhdoom, S. I., Aziz, T., Mohamed, A. A., Sameeh, M. Y., Alruways, M. W., Dablool, A. S., Almalki, A. A., Alamri, A. S., Alhomrani, M. (2022). Characterization and Evaluation of the Antioxidant, Antidiabetic, Anti-Inflammatory, and Cytotoxic Activities of Silver Nanoparticles Synthesized Using Brachychiton populneus Leaf Extract. Processes, 10(8), 1521. https://doi.org/10.3390/pr10081521
• Nayak, D., Pradhan, S., Ashe, S., Rauta, P. R., Nayak, B. (2015). Biologically synthesised silver nanoparticles from three diverse family of plant extracts and their anticancer activity against epidermoid A431 carcinoma. Journal of Colloid and Interface Science, 457, 329-338. https://doi.org/10.1016/j.jcis.2015.07.012
• Nayak, S., Bhat, M. P., Udayashankar, A. C., Lakshmeesha, T. R., Geetha, N., Jogaiah, S. (2020). Biosynthesis and characterization of Dillenia indica-mediated silver nanoparticles and their biological activity. Applied Organometallic Chemistry, 34, e5567. https://doi.org/10.1002/aoc.5567
• Palithya, S., Gaddam, S. A., Kotakadi, V. S., Penchalaneni, J., Golla, N., Krishna, S. B. N., Naidu, C. V. (2021). Green synthesis of silver nanoparticles using flower extracts of Aerva lanata and their biomedical applications. Particulate Science and Technology, 40(1), 84-96. https://doi.org/10.1080/02726351.2021.1919259
• Pallela, P. N. V. K., Ummey, S., Ruddaraju, L. K., Pammi, S. V. N., Yoon, S. G. (2018). Ultra Small, mono dispersed green synthesized silver nanoparticles using aqueous extract of Sida cordifolia plant and investigation of antibacterial activity. Microbial Pathogenesis, 124, 63-69. https://doi.org/10.1016/j.micpath.2018.08.026
• Paosen, S., Saising, J., Wira Septama, A., Piyawan Voravuthikunchai, S. (2017). Green synthesis of silver nanoparticles using plants from Myrtaceae family and characterization of their antibacterial activity. Materials Letters, 209, 201-206. https://doi.org/10.1016/j.matlet.2017.07.102
• Patil, P. M., Kang, M. -j, Niyonizigiye, I., Singh, A., Kim, J.-O., Seo, Y. B., Kim, G. -D. (2019). Extracellular synthesis of gold nanoparticles using the marine bacterium Paracoccus haeundaensis BC74171T and evaluation of their antioxidant activity and antiproliferative effect on normal and cancer cell lines. Colloids and Surfaces B: Biointerfaces, 183, 110455. https://doi.org/10.1016/j.colsurfb.2019.110455
• Philip, D. (2011). Mangifera Indica leaf-assisted biosynthesis of well-dispersed silver nanoparticles. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 78(1), 327-331. https://doi.org/10.1016/j.saa.2010.10.015
• Salem, S. S., Fouda, A. (2021). Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: an Overview. Biological Trace Element Research, 199, 344-370. https://doi.org/10.1007/s12011-020-02138-3
• Shruthi, G., Prasad, K. S., Vinod, T. P., Balamurugan, V., Shivamallu, C. (2017). Green Synthesis of Biologically Active Silver Nanoparticles through a Phyto-Mediated Approach Using Areca catechu Leaf Extract. ChemistrySelect, 2, 10354-10359. https://doi.org/10.1002/slct.201702257
• Singh, A., Dar, M. Y., Joshi, B., Sharma, B., Shrivastava, S., Shukla, S. (2018). Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments. Toxicology Reports, 5, 333–342. https://doi.org/10.1016/j.toxrep.2018.02.013
• Sökmen, M. F., İspiroğlu, M., Gişi, K., Bahar, A. Y., Kurutaş, E. B., Kantarçeken, B. (2023). Effects of Arum dioscoridis Extract on Hepatic Toxicity Caused by Thioacetamide in Rats. Turkish Journal of Gastroenterology, 34, 354-261. https://doi.org/10.5152/tjg.2023.211051
• Song, J. Y., Kim, B. S. (2009). Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess and Biosystems Engineering, 32, 79-84 https://doi.org/10.1007/s00449-008-0224-6
• Tamboli, D. P., Lee, D. S. (2013). Mechanistic antimicrobial approach of extracellularly synthesized silver nanoparticles against gram positive and gram negative bacteria. Journal of Hazardous Materials, 260, 878-84. https://doi.org/10.1016/j.jhazmat.2013.06.003
• Tufail, S., Ali, Z., Hanif, S., Sajjad, A., Zia, M. (2022). Synthesis and morphological & biological characterization of Campsis radicans and Cascabela thevetia petals derived silver nanoparticles. Biochemical Systematics and Ecology, 105, 104526. https://doi.org/10.1016/j.bse.2022.104526
• Vanlalveni, C., Lallianrawna, S., Biswas, A., Selvaraj, M., Changmai, B., Rokhum, S. L. (2021). Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature. RSC Advances, 11(5), 2804-2837. https://doi.org/10.1039/D0RA09941D
• Wang, J., Che, B., Zhang, L. W., Dong, G., Luo, Q., Xin, L. (2016). Comparative genotoxicity of silver nanoparticles in human liver HepG2 and lung epithelial A549 cells. Journal of Applied Toxicology, 37(4), 495-501. https://doi.org/10.1002/jat.3385
• Yabalak, E. (2018). Radical Scavenging Activity and Chemical Composition of Methanolic Extract from Arum dioscoridis SM. var. dioscoridis and Determination of Its Mineral and Trace Elements. Journal of the Turkish Chemical Society Section A: Chemistry, 5(1), 205-18. https://doi.org/10.18596/jotcsa.350370
• Younas, M., Rasool, M. H., Khurshid, M., Khan, A., Nawaz, M. Z., Ahmad, I., Lakhan, M. N. (2023). Moringa oleifera leaf extract mediated green synthesis of silver nanoparticles and their antibacterial effect against selected gram-negative strains. Biochemical Systematics and Ecology, 107, 104605. https://doi.org/10.1016/j.bse.2023.104605
• Zhang, X.-F., Liu, Z.-G., Shen, W., Gurunathan, S. (2016). Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. International Journal of Molecular Sciences, 17(9), 1534. https://doi.org/10.3390/ijms17091534
• Zubair, M., Azeem, M., Mumtaz, R., Younas, M., Adrees, M., Zubair, E., Khalid, A., Hafeez, F., Rizwan, M., Ali, S. (2022). Green synthesis and characterization of silver nanoparticles from Acacia nilotica and their anticancer, antidiabetic and antioxidant efficacy. Environmental Pollution, 304, 119249. https://doi.org/10.1016/j.envpol.2022.119249