Determination of the effects of drought stress on Aronia melanocarpa cv. Nero in vitro conditions
Yıl 2024,
Cilt: 8 Sayı: 4, 760 - 767
Heydem Ekinci
,
Necla Şaşkın
,
Birgül Dikmetaş
,
Bekir Erol Ak
Öz
Drought stress is a significant threat to plant cultivation in arid and semi-arid regions, negatively affecting growth and leading to plant mortality. This study evaluated the in vitro drought tolerance of Aronia melanocarpa ‘Nero’ by exposing micropropagated plantlets to nutrient media containing different concentrations of PEG-8000 (0%, 1%, and 2%) during the rooting stage. Results showed that increasing PEG concentrations led to a reduction in survival, rooting, plantlet growth, and physiological parameters. The 0% PEG treatment resulted in the highest survival rate (95.83%), root number, and chlorophyll content, while the 2% PEG treatment significantly hindered these parameters. These findings indicate that Aronia melanocarpa 'Nero' is sensitive to drought stress, with reduced growth and physiological activity under higher PEG concentrations.
Kaynakça
- Albiski, F., Najla, S., Sanoubar, R., Alkabani, N. & Murshed, R. (2012). In vitro screening of potato lines for drought tolerance. Physiology and Molecular biology of plants, 18, 315-321. https://doi.org/10.1007/s12298-012-0127-5
- Almokar, H.M.M. & Pirlak, L. (2018). Propagation of Aronia (Aronia melanocarpa) with tissue culture. Selcuk Journal of Agriculture and Food Sciences, 32(3), 549-558. https://doi.org/10.15316/SJAFS.2018.136
- Altıncı, N.T. & Cangi, R. (2019). Drought Tolerance of Some Wine Grape Cultivars under In Vitro Conditions. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 36(2), 145-152. https://doi.org/10.13002/jafag4633
- Anjum, S.A., Xie, X.Y., Wang, L. C., Saleem, M.F., Man, C. & Lei, W. (2011). Morphological, physiological and biochemical responses of plants to drought stress. African journal of agricultural research, 6(9), 2026-2032. https://doi.org/10.5897/AJAR10.027
- Babu, G.A., Mosa Christas, K., Kowsalya, E., Ramesh, M., Sohn, S. I., & Pandian, S. (2022). Improved sterilization techniques for successful in vitro micropropagation. In Commercial Scale Tissue Culture for Horticulture and Plantation Crops (pp. 1-21). Singapore: Springer Nature Singapore.
- Bilir Ekbic, H., Gecene, İ. & Ekbic, E. (2022). Tilki Üzümlerinin (Vitis labrusca L.) Kuraklık Stresine Toleransının in vitro PEG Uygulaması ile Belirlenmesi. Erwerbs-Obstbau, 64 (Ek 1), 87-94 (in Turkish). https://doi.org/10.29278/azd.1059957
- Celik, H., Karabulut, B., & Uray, Y. (2022). Growth-Development, yield and quality characteristics of Aronia varieties grown in pots. International Journal of Innovative Approaches in Agricultural Research, 6(3), 246-254. https://doi.org/10.29329/ijiaar.2022.475.8
- Dobránszki, J., Magyar-Tábori, K., & Takács-Hudák, Á. (2003). Growth and developmental responses of potato to osmotic stress under in vitro conditions. Acta biologica hungarica, 54, 365-372.
- Ekinci, H., Saskin, N., Korkmaz, Ş., Ak, B.E. & Aydınlık, Y. (2024). The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13542-13542. https://doi.org/10.15835/nbha52113542
- Gecene, İ. (2020). Kokulu Üzümün (Vitis labrusca L.) Kuraklık Stresine Toleransının Peg Uygulamasıyla In Vitro Koşullarda Belirlenmesi. Ordu Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Yüksek Lisans Tezi, Ordu, Türkiye, 52 s.
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- Hancı, F. & Cebeci, E. (2014). Investigation of proline, chlorophyll and carotenoids changes under drought stress in some onion (Allium cepa L.) cultivars. Türk Tarım ve Doğa Bilimleri Dergisi, 1(Özel Sayı-2), 1499-1504.
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- Kabay, T. & Şensoy, S. (2016). Enzyme, chlorophyl and ion changes in some common bean genotypes by drought stress. Yuzuncu Yıl University Journal of Agricultural Sciences, 26(3), 380-395.
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- Martínez-Santos, E., Cruz-Cruz, C.A., Spinoso-Castillo, J.L. & Bello-Bello, J.J. (2021). In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress. Scientific Reports, 11(1), 22611. https://doi.org/10.1038/s41598-021-02207-0
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- Mohsen, A.T., Stino, R.G., Abd Allatif, A.M. & Zaid, N.M. (2020). In vitro evaluation of some grapevine rootstocks grown under drought stress. Plant Archives, 20(1), 1029-1034.
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Yıl 2024,
Cilt: 8 Sayı: 4, 760 - 767
Heydem Ekinci
,
Necla Şaşkın
,
Birgül Dikmetaş
,
Bekir Erol Ak
Kaynakça
- Albiski, F., Najla, S., Sanoubar, R., Alkabani, N. & Murshed, R. (2012). In vitro screening of potato lines for drought tolerance. Physiology and Molecular biology of plants, 18, 315-321. https://doi.org/10.1007/s12298-012-0127-5
- Almokar, H.M.M. & Pirlak, L. (2018). Propagation of Aronia (Aronia melanocarpa) with tissue culture. Selcuk Journal of Agriculture and Food Sciences, 32(3), 549-558. https://doi.org/10.15316/SJAFS.2018.136
- Altıncı, N.T. & Cangi, R. (2019). Drought Tolerance of Some Wine Grape Cultivars under In Vitro Conditions. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 36(2), 145-152. https://doi.org/10.13002/jafag4633
- Anjum, S.A., Xie, X.Y., Wang, L. C., Saleem, M.F., Man, C. & Lei, W. (2011). Morphological, physiological and biochemical responses of plants to drought stress. African journal of agricultural research, 6(9), 2026-2032. https://doi.org/10.5897/AJAR10.027
- Babu, G.A., Mosa Christas, K., Kowsalya, E., Ramesh, M., Sohn, S. I., & Pandian, S. (2022). Improved sterilization techniques for successful in vitro micropropagation. In Commercial Scale Tissue Culture for Horticulture and Plantation Crops (pp. 1-21). Singapore: Springer Nature Singapore.
- Bilir Ekbic, H., Gecene, İ. & Ekbic, E. (2022). Tilki Üzümlerinin (Vitis labrusca L.) Kuraklık Stresine Toleransının in vitro PEG Uygulaması ile Belirlenmesi. Erwerbs-Obstbau, 64 (Ek 1), 87-94 (in Turkish). https://doi.org/10.29278/azd.1059957
- Celik, H., Karabulut, B., & Uray, Y. (2022). Growth-Development, yield and quality characteristics of Aronia varieties grown in pots. International Journal of Innovative Approaches in Agricultural Research, 6(3), 246-254. https://doi.org/10.29329/ijiaar.2022.475.8
- Dobránszki, J., Magyar-Tábori, K., & Takács-Hudák, Á. (2003). Growth and developmental responses of potato to osmotic stress under in vitro conditions. Acta biologica hungarica, 54, 365-372.
- Ekinci, H., Saskin, N., Korkmaz, Ş., Ak, B.E. & Aydınlık, Y. (2024). The effect of sodium nitroprusside on the vegetative development of Aronia melanocarpa [Michx.] Elliot under in vitro conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13542-13542. https://doi.org/10.15835/nbha52113542
- Gecene, İ. (2020). Kokulu Üzümün (Vitis labrusca L.) Kuraklık Stresine Toleransının Peg Uygulamasıyla In Vitro Koşullarda Belirlenmesi. Ordu Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Yüksek Lisans Tezi, Ordu, Türkiye, 52 s.
- Gomez, K.A., & Gomez, A. A. (1984) Statistical procedures for agricultural research, 2nd edn. John Wiley & Sons, New York, 680 pp.
- Granato, D., Santos, J. S., Escher, G.B., Ferreira, B.L. & Maggio, R.M. (2018). Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. Trends in Food Science & Technology, 72, 83-90. https://doi.org/10.1016/j.tifs.2017.12.006
- Gullapalli, R.P. & Mazzitelli, C.L. (2015). Polyethylene glycols in oral and parenteral formulations—A critical review. International Journal of Pharmaceutics, 496(2), 219-239. https://doi.org/10.1016/j.ijpharm.2015.11.015
- Hancı, F. & Cebeci, E. (2014). Investigation of proline, chlorophyll and carotenoids changes under drought stress in some onion (Allium cepa L.) cultivars. Türk Tarım ve Doğa Bilimleri Dergisi, 1(Özel Sayı-2), 1499-1504.
- Herzberger, J., Niederer, K., Pohlit, H., Seiwert, J., Worm, M., Wurm, F.R. & Frey, H. (2016). Polymerization of ethylene oxide, propylene oxide, and other alkylene oxides: synthesis, novel polymer architectures, and bioconjugation. Chemical reviews, 116(4), 2170-2243. https://doi.org/10.1021/acs.chemrev.5b00441
- Jones, H. G. & Corlett, J. E. (1992). Current topics in drought physiology. The Journal of Agricultural Science, 119(3), 291-296. https://doi.org/10.1017/S0021859600012144
- Kabay, T. & Şensoy, S. (2016). Enzyme, chlorophyl and ion changes in some common bean genotypes by drought stress. Yuzuncu Yıl University Journal of Agricultural Sciences, 26(3), 380-395.
- Kapci, B., Neradová, E., Čížková, H., Voldřich, M., Rajchl, A. & Capanoglu, E. (2013). Investigating the antioxidant potential of chokeberry (Aronia melanocarpa) products. Journal of Food and Nutrition Research, 52, 219–229.
- Kara, Z., Yazar, K., Ekinci, H., Doğan, O. & Özer, A. (2022). The Effects of Ortho Silicone Applications on the Acclimatization Process of Grapevine Rootstocks. Selcuk Journal of Agriculture and Food Sciences, 36(2), 233-237. https://doi.org/10.15316/SJAFS.2022.030
- Kocaçalışkan, İ. (2003). Bitki fizyolojisi. DPÜ Fen-Edebiyat Fakültesi Yayını.
- Lee, I.; Yang, J. (2009). Common clustering algorithms. In Comprehensive Chemometrics (pp. 577–618). Elsevier: Oxford, UK. https://doi.org/10.1016/B978-044452701-1.00064-8
- Long, S.P., ZHU, X. G., Naidu, S.L., & Ort, D.R. (2006). Can improvement in photosynthesis increase crop yields?. Plant, cell & environment, 29(3), 315-330. https://doi.org/10.1111/j.1365-3040.2005.01493.x
- Lu, H.B., Qiao, Y.M., Gong, X.C., Li, H.Q., Zhang, Q., Zhao, Z.H. & Meng, L.L. (2015). Influence of drought stress on the photosynthetic characteristics and dry matter accumulation of hybrid millet. Photosynthetica, 53(2), 306-311. https://doi.org/10.1007/s11099-015-0120-7
- Mahajan, S. & Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Archives of biochemistry and biophysics, 444(2), 139-158. https://doi.org/10.1016/j.abb.2005.10.018
- Manivannan, P., Jaleel, C. A., Sankar, B., Kishorekumar, A., Somasundaram, R., Lakshmanan, G.A. & Panneerselvam, R. (2007). Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids and Surfaces B: Biointerfaces, 59(2), 141-149. https://doi.org/10.1016/j.colsurfb.2007.05.002
- Martínez-Santos, E., Cruz-Cruz, C.A., Spinoso-Castillo, J.L. & Bello-Bello, J.J. (2021). In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress. Scientific Reports, 11(1), 22611. https://doi.org/10.1038/s41598-021-02207-0
- Mengesha, B., Mekbib, F. & Abraha, E. (2016). In vitro screening of cactus [Opuntia ficus-indicia (L.) Mill] genotypes for drought tolerance. American Journal of Plant Sciences, 7(13), 1741. https://doi.org/10.4236/ajps.2016.713163
- Mese, N. & Tangolar, S. (2019). Bazı Amerikan asma anaçlarının kurağa dayanımının in vitro’da polietilen glikol kullanılarak belirlenmesi. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(3), 466-475 (in Turkish). https://doi.org/10.29133/yyutbd.559174
- Mohsen, A.T., Stino, R.G., Abd Allatif, A.M. & Zaid, N.M. (2020). In vitro evaluation of some grapevine rootstocks grown under drought stress. Plant Archives, 20(1), 1029-1034.
- Nas, Z., Esitken, A., & Pirlak, L. (2023). Determination of Plant Regeneration Protocol of “Viking” Aronia Cultivar in the In Vitro Conditions. PREPRINT (Version 1) available at Research Square, https://doi.org/10.21203/rs.3.rs-3004327/v1
- Negreanu-Pirjol, B.S., Oprea, O.C., Negreanu-Pirjol, T., Roncea, F.N., Prelipcean, A.M., Craciunescu, O., ... & Popoviciu, D.R. (2023). Health Benefits of Antioxidant Bioactive Compounds in the Fruits and Leaves of Lonicera caerulea L. and Aronia melanocarpa (Michx.) Elliot. Antioxidants, 12(4), 951. https://doi.org/10.3390/antiox12040951
- Nurcahyani, E., Sumardi, S. & Hardoko, I.Q. (2019). Analysis of chlorophyll Phalaenopsis amabilis (L.) Bl. results of the resistance to Fusarium oxysporum and drought stress. IOSR Journal of Research & Method in Education (IOSR-JRME), 12(11-I), 41-46. https://doi.org/10.9790/2380-1211014146
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