TİCARİ PROBİYOTİK KÜLTÜR KULLANILAN EV YAPIMI YOĞURT ÜRETİMİNİN VE ÖZELLİKLERİNİN ARAŞTIRILMASI

Year 2020, Volume: 45 Issue: 4, 814 - 824, 21.06.2020

Özüm Özoğlu , Rukiye Çolak Şaşmazer , Zerrin Erginkaya , Mihriban Korukluoğlu

https://doi.org/10.15237/gida.GD20028

Cited By: 2

Abstract

Bu çalışmada ticari probiyotik kültür kullanılarak probiyotik ev yapımı yoğurt yapılmış ve kalite parametreleri depolamanın 1., 5., 10. ve 15. günlerinde araştırılmıştır. Bu amaçla probiyotik ve probiyotik olmayan ticari yoğurtlar da kontrol grubu olarak araştırılmıştır. Elde edilen sonuçlara göre; toplam laktik asit bakterisi, Lactobacillus acidophilus ve Bifidobacterium animalis ssp. lactis ve pH değerleri, tüm numuneler için saklama süresinde önemli ölçüde değişmemiştir (P >0.05). Tüm örneklerde önemli sayıda koliform, küf ve/veya maya gözlenmemiştir (<10 KOB/g). Yoğurt yapımında kullanılan sütler arasında kuru madde değerleri benzer bulunurken; yağ ve protein değerleri farklılık göstermiştir. Ev yapımı yoğurt, panellistler tarafından çok tercih edilen olmamıştır, ancak değerlendirmede diğer yoğurtlarla kıyaslandığında önemli ölçüde farklılık göstermemiştir (P >0.05).

Keywords

Ev Yapımı Yoğurt, Probiyotik, Starter Kültür

INVESTIGATION OF PRODUCTION AND CHARACTERISTICS OF HOMEMADE YOGURT BY USING A COMMERCIAL PROBIOTIC CULTURE

Abstract

In this study, probiotic homemade yogurt was made by using a commercial probiotic culture and its quality parameters were investigated on the 1st, 5th, 10th and 15th days of storage. For this purpose, probiotic and non-probiotic commercial yogurts were also investigated as control groups. According to the results obtained; level of total lactic acid bacteria, Lactobacillus acidophilus and Bifidobacterium animalis ssp. lactis and pH values were not significantly changed in storage time for all samples (P >0.05). Any significant number of coliforms, mould or/and yeast were not observed in all samples (<10 CFU/g). Dry matter values were found similar among the milks used in yogurt makings, while fat and protein values showed difference. The homemade yogurt was not highly preferred by the panellists; however, it did not show any significant difference in the evaluation compared to the other yogurts (P >0.05).

Keywords

Homemade Yogurt, Probiotic, Starter Culture

References

• Abhisingha, M., Dumnil, J., and Pitaksutheepong, C. (2018). Selection of potential probiotic Lactobacillus with inhibitory activity against Salmonella and fecal coliform bacteria. Probiotics Antimicrob. Proteins 10(2): 218–227. doi:10.1007/s12602-017-9304-8.
• Akalın, A. S., Unal, G., Dinkci, N., and Hayaloglu, A. A. (2012). Microstructural, textural, and sensory characteristics of probiotic yogurts fortified with sodium calcium caseinate or whey protein concentrate. J. Dairy Sci. 95(7): 3617–3628. doi:10.3168/jds.2011-5297.
• Akpinar, A., Yerlikaya, O., and Kiliç, S. (2011). Antimicrobial activity and antibiotic resistance of Lactobacillus delbrueckii ssp . bulgaricus and Streptococcus thermophilus strains isolated from Turkish homemade yoghurts. African J. Microbiol. Res. 5(6): 675–682. doi:10.5897/AJMR10.835.
• Anonymous (2009). Türk gıda kodeksi. Fermente süt ürünleri tebliği (2009/25). Tarım ve Köyişleri Bakanlığı. 16 Şubat 2009 tarih ve 27143 sayılı Resmî Gazete, Ankara.
• Aslim, B., Beyatli, Y., and Yuksekdag, Z. N. (2006). Productions and monomer compositions of exopolysaccharides by Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains isolated from traditional home-made yoghurts and raw milk. Int. J. Food Sci. Technol. 41(8): 973–979. doi:10.1111/j.1365-2621.2005.01155.x.
• Batista, A. L. D., Silva, R., Cappato, L. P., Almada, C. N., Garcia, R. K. A., Silva, M. C., et al. (2015). Quality parameters of probiotic yogurt added to glucose oxidase compared to commercial products through microbiological, physical-chemical and metabolic activity analyses. Food Res. Int. 77: 627–635. doi:10.1016/j.foodres.2015.08.017.
• Cruz, A. G., Castro, W. F., Faria, J. A. F., Lollo, P. C. B., Amaya-Farfán, J., Freitas, M. Q., et al. (2012). Probiotic yogurts manufactured with increased glucose oxidase levels: Postacidification, proteolytic patterns, survival of probiotic microorganisms, production of organic acid and aroma compounds. J. Dairy Sci. 95(5): 2261–2269. doi:10.3168/jds.2011-4582.
• Çayır, M. S., Şahan, N. (2007). Probiyotik kültür kullanılarak üretilen kayısı katkılı yoğurtların fizikokimyasal özellikleri. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı Yüksek Lisans Tezi, Adana, Türkiye, 75 s.
• Çelik, H., Durak, Y., Uysal, A. (2016). Bazı ticari ve ev yapımı yoğurtlardan izole edilen laktik asit bakterilerinin antibiyotik duyarlılıkları. Selçuk Üniversitesi Fen Fakültesi Fen Derg. 42(2): 149–160.
• Demirkaya, A. K., Ceylan, Z. G. (2013). Bilecik’te tüketime sunulan yoğurtların kimyasal ve mikrobiyolojik kalitesinin araştırılması. Atatürk Üniversitesi Vet. Bil. Derg. 8(3): 202–209.
• Dias, M. de L. L. A., Salgado, S. M., Guerra, N. B., Livera, A. V. S., Andrade, S. A. C., Ximenes, G. N. da C. (2013). Phisicochemical, sensory, and microbiological evaluation and development of symbiotic fermented drink. Food Sci. Technol. 33(4): 805–811. doi:10.1590/S0101-20612013000400030.
• Durak, Y., Keleş, F., Uysal, A., Aladağ, M. O. (2008). Konya yöresi taze ev yapımı yoğurtların mikrobiyolojik özelliklerinin araştırılması. Selçuk Üniversitesi Ziraat Fakültesi Derg. 22(44): 113–117.
• Hakimi, S., Rohani, J. bin M., Hemmatboland, M. (2013). Application of design of experiments to homemade yogurt production process. International Conference on Robust Quality Engineering, Taguchi, S. (chief ed.), Volume 1, UTM Razak School, the Malaysia, pp.75–79. doi:10.1016/S0090-8258(15)01318-9.
• Helrich, K. (1990). AOAC: official methods of analysis. 15th Edition, Association of Official Agricultural Chemists, Virginia, the USA, 552 p. doi:10.1371/journal.pone.0013135.
• Herdem, A. (2006). Farklı yörelerden toplanan geleneksel yöntemle üretilen yoğurt örneklerinin bazı niteliklerinin belirlenmesi. Selçuk Üniversitesi Fen Bilimleri Enstitüsü Gıda Mühendisliği Anabilim Dalı Yüksek Lisans Tezi, Konya, Türkiye, 85 s.
• ISO 20128:2006 - Milk products -- Enumeration of presumptive Lactobacillus acidophilus on a selective medium -- Colony-count technique at 37 degrees C. https://www.iso.org/standard/35292.html (Accessed 31 August 2018).
• ISO 29981:2010 - Milk products -- Enumeration of presumptive bifidobacteria -- Colony count technique at 37 degrees C. https://www.iso.org/standard/45765.html (Accessed 31 August 2018).
• ISO 4832:2006 - Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of coliforms -- Colony-count technique. https://www.iso.org/standard/38282.html (Accessed 31 August 2018).
• ISO 6611:2004 - Milk and milk products -- Enumeration of colony-forming units of yeasts and/or moulds -- Colony-count technique at 25 degrees C. https://www.iso.org/standard/40473.html (Accessed 31 August 2018). Isolauri, E., Sütas, Y., Kankaanpää, P., Arvilommi, H., and Salminen, S. (2001). Probiotics: effects on immunity. Am. J. Clin. Nutr. 73(2): 444–450.
• Kailasapathy, K. (2006). Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. LWT - Food Sci. Technol. 39(10): 1221–1227. doi:10.1016/j.lwt.2005.07.013.
• Kaya, Z., Yiğit, Ö., Erol, M., Gayret, Ö. B. (2016). Altı-yirmi dört ay arası yaş grubunda beslenmeyle ilgili anne ve babalarin bilgi ve deneyimlerinin değerlendirilmesi. Haseki Tip Bul. 54(2): 70–75. doi:10.4274/haseki.2756.
• Khan, S. U. (2014). Probiotics in dairy foods: a review. Nutr. Food Sci. 44(1): 71–88. doi:10.1108/NFS-04-2013-0051.
• Kleyn, D. H., Lynch, J. M., Barbano, D. M., Bloom, M. J., and Mitchell, M. W. (2001). Determination of fat in raw and processed milks by the gerber method: collaborative study. J. AOAC Int. 84(5): 1499–1508.
• Lawless, H. T., Heymann, H. (2003). Sensory Evaluation of Food. 2nd Edition, Springer Science+Business Media, New York, the USA, 596p. ISBN: 9781441964878 doi:10.1007/978-1-4419-6488-5.
• Lee, P. R., Boo, C. X., and Liu, S. Q. (2013). Fermentation of coconut water by probiotic strains Lactobacillus acidophilus L10 and Lactobacillus casei L26. Ann. Microbiol. 63(4): 1441–1450. doi:10.1007/s13213-013-0607-z.
• Lourens-Hattingh, A., Viljoen, B. C. (2001). Yogurt as probiotic carrier food. Int. Dairy J. 11(1–2): 1–17. doi:10.1016/S0958-6946(01)00036-X.
• Mohammadi, R., Yousefi, M., Sarlak, Z., Shah, N. P., Mortazavian, A. M., Sadeghi, E., et al. (2017). Influence of commercial culture composition and cow milk to soy milk ratio on the biochemical, microbiological, and sensory characteristics of a probiotic fermented composite drink. Food Sci. Biotechnol. 26(3): 749–757. doi:10.1007/s10068-017-0097-z.
• Moreno, Y., Collado, M. C., Ferrús, M. A., Cobo, J. M., Hernández, E., and Hernández, M. (2006). Viability assessment of lactic acid bacteria in commercial dairy products stored at 4 °C using LIVE/DEAD® BacLightTM staining and conventional plate counts. Int. J. Food Sci. Technol. 41(3): 275–280. doi:10.1111/j.1365-2621.2005.01060.x.
• Quigley, E. M. M. (2010). Prebiotics and probiotics; modifying and mining the microbiota. Pharmacol. Res. 61(3): 213–218. doi:10.1016/j.phrs.2010.01.004.
• Quin, C., Estaki, M., Vollman, D. M., Barnett, J. A., Gill, S. K., and Gibson, D. L. (2018). Probiotic supplementation and associated infant gut microbiome and health: A cautionary retrospective clinical comparison. Sci. Rep. 8(1): 1–16. doi:10.1038/s41598-018-26423-3.
• Reid, G., Sanders, M. E., Gaskins, H. R., Gibson, G. R., Mercenier, A., Rastall, R., Roberfroid, M., Rowland, I., Cherbut, C., and Klaenhammer, T. R. (2003). New scientific paradigms for probiotics and prebiotics. J Clin Gastroenterol 37(2): 105–118.
• Reid, G. (2015). The growth potential for dairy probiotics. Int. Dairy J. 49: 16–22. doi:10.1016/j.idairyj.2015.04.004.
• Shima, R., Salina, H. F., Masniza, M., and Atiqah, H. (2012). Viability of lactic acid bacteria in home made yogurt containing sago starch oligosaccharides. Int. J. Basic Appl. Sci. 12(1): 58–62.
• Shori, A. B. (2015). The potential applications of probiotics on dairy and non-dairy foods focusing on viability during storage. Biocatal. Agric. Biotechnol. 4(4): 423–431. doi:10.1016/j.bcab.2015.09.010.
• Simone, M., Gozzoli, C., Quartieri, A., Mazzola, G., Di Gioia, D., Amaretti, A., et al. (2014). The probiotic bifidobacterium breve B632 inhibited the growth of Enterobacteriaceae within colicky infant microbiota cultures. Biomed Res. Int. 2014. doi:10.1155/2014/301053.
• Tatsadjieu, L. N., Tchikoua, R., and Funtong, C. M. M. (2016). Antifungal activity of lactic acid bacteria against molds isolated from corn and fermented corn paste. Am. J. Microbiol. Res. 4(4): 90–100. doi:10.12691/AJMR-4-4-1.
• Tripathi, M. K., and Giri, S. K. (2014). Probiotic functional foods: survival of probiotics during processing and storage. J. Funct. Foods 9(1): 225–241. doi:10.1016/j.jff.2014.04.030.
• Turgut, T., and Cakmakci, S. (2018). probiotic strawberry yogurts: microbiological, chemical and sensory properties. Probiotics Antimicrob. Proteins 10(1): 64–70. doi:10.1007/s12602-017-9278-6.
• Yerlikaya, O. (2014). Starter cultures used in probiotic dairy product preparation and popular probiotic dairy drinks. Food Sci. Technol. 34(2): 221–229. doi:10.1590/fst.2014.0050.