Abstract
Penicillium roqueforti is a filamentous fungus used as secondary starter culture in mold-ripening of cheese. In facilities using starter cultures, P. roqueforti is added directly in milk or introduced by spraying on the cheese curd. If a commercial starter is not used, the fungus can spontaneously grow on cheese through contamination from the environment. In recent years, studies on morphological, metabolic, and genetic characteristics of P. roqueforti have been conducted. New findings have been reported on the adaptation of P. roqueforti that originates from caves or cellars, to cheese matrix and the domestication process that has been going on for centuries. In addition, this species was found to be able to reproduce sexually, which is of great biotechnological importance. In this review, taxonomic, morphological, genetic, and metabolic features of P. roqueforti are presented, the use of these characteristics in cheese production and the biotechnological potential of P. roqueforti is emphasized.